The plant lectin Bandeiraea simplicifolia I-B4 identifies a subpopulation of small diameter primary sensory neurones which innervate the skin in the rat

Plenderleith, Mark B.; Snow, Peter J.

doi:10.1016/0304-3940(93)90787-l

Cited by 84 publications

(65 citation statements)

References 14 publications

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“…The results of this study confirm previous reports of BSI-B 4 binding in the rat, mouse, and rabbit (Streit et al, 1985;Kruger, 1988, 1990;Plenderleith et al, 1992;Plenderleith and Snow, 1993;Kitchener et al, 1994;Wang et al, 1994), and are entirely consistent with studies reporting BSI-B 4 binding in dorsal root ganglia and spinal cord of the chicken (Scott et al, 1990), echidna (Ashwell and Zhang, 1997), opossum (Knott et al, 1999), and rhesus monkey (Tong et al, 1999). Furthermore, recent studies have reported BSI-B 4 binding sites on human primary sensory neurones collected either post-mortem or from patients undergoing surgery (Yiangou et al, 2000;Alavi et al, 2001).…”

Section: Discussionsupporting

confidence: 82%

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Gerke

Plenderleith

2002

Anat. Rec.

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The purpose of the present study was to investigate the binding patterns of the plant lectin Bandeiraea simplicifolia I-isolectin B 4 (BSI-B 4 ) to sensory neurones in seven mammalian species. The dorsal root ganglia and spinal cords of three rats, mice, guinea pigs, rabbits, flying foxes, cats, and marmoset monkeys were screened for BSI-B 4 using lectin histochemistry. BSI-B 4 binding was associated with the soma of predominantly smalldiameter primary sensory neurones in the dorsal root ganglia and their axon terminals within laminae I and II of the superficial dorsal horn in all seven species. The similarities of lectin binding patterns in each of these species suggest that the glycoconjugate to which BSI-B 4 binds has a ubiquitous distribution in mammals, and supports the proposal that this lectin may preferentially bind to a subpopulation of sensory neurones with a similar functional role in each of these species. Anat Rec 268: 105-114, 2002.

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Section: Discussionsupporting

confidence: 82%

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Gerke

Plenderleith

2002

Anat. Rec.

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“…The reason for this is unclear, but it may indicate that some of the smallest neurons that are strongly IB4ϩ may have been underrepresented in the physiological recordings because of greater ease of recording from larger neurons. In addition, receptive fields of some of our dye-injected neurons may have been in muscle, and very few muscle afferents are reported to be IB4ϩ (Plenderleith and Snow, 1993;Thornton et al, 2005). Comparison of Figure 1B (not dye injected) and D (dye injected) shows similar size distributions, with all strongly IB4ϩ neurons having small somata and C-fibers (Fig.…”

Section: Ib4 Binding In Identified Drg Neuronsmentioning

confidence: 66%

Intense Isolectin-B4 Binding in Rat Dorsal Root Ganglion Neurons Distinguishes C-Fiber Nociceptors with Broad Action Potentials and High Nav1.9 Expression

et al. 2006

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Binding to isolectin-B4 (IB4) and expression of tyrosine kinase A (trkA) (the high-affinity NGF receptor) have been used to define two different subgroups of nociceptive small dorsal root ganglion (DRG) neurons. We previously showed that only nociceptors have high trkA levels. However, information about sensory and electrophysiological properties in vivo of single identified IB4-binding neurons, and about their trkA expression levels, is lacking. IB4-positive (IB4ϩ) and small dark neurons had similar size distributions. We examined IB4-binding levels in Ͼ120 dye-injected DRG neurons with sensory and electrophysiological properties recorded in vivo. Relative immunointensities for trkA and two TTX-resistant sodium channels (Nav1.8 and Nav1.9) were also measured in these neurons. IB4ϩ neurons were classified as strongly or weakly IB4ϩ.All strongly IB4ϩ neurons were C-nociceptor type (C-fiber nociceptive or unresponsive). Of 32 C-nociceptor-type neurons examined, ϳ50% were strongly IB4ϩ, ϳ20% were weakly IB4ϩ and ϳ30% were IB4Ϫ. A␦ low-threshold mechanoreceptive (LTM) neurons were weakly IB4ϩ or IB4Ϫ. All 33 A-fiber nociceptors and all 44 A␣/␤-LTM neurons examined were IB4Ϫ. IB4ϩ compared with IB4Ϫ C-nociceptor-type neurons had longer somatic action potential durations and rise times, slower conduction velocities, more negative membrane potentials, and greater immunointensities for Nav1.9 but not Nav1.8. Immunointensities of IB4 binding in C-neurons were positively correlated with those of Nav1.9 but not Nav1.8. Of 23 C-neurons tested for both trkA and IB4, ϳ35% were trkAϩ/IB4ϩ but with negatively correlated immunointensities; 26% were IB4ϩ/trkAϪ, and 35% were IB4Ϫ/trkAϩ. We conclude that strongly IB4ϩ DRG neurons are exclusively C-nociceptor type and that high Nav1.9 expression may contribute to their distinct membrane properties.

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“…A vast majority of sensory neurons that project into the pancreas are calcitonin gene-related peptide positive (21,22), whereas virtually all spinal sensory neurons that express GFR␣2 are CGRP Ϫ and do not project into the pancreas (M.S.A., unpublished data) (23). In addition, although some sensory neurons in the nodose ganglion project into the islets (24), few nodose ganglion neurons express GFR␣2 (25).…”

Section: Indirect Evidence Suggests That Islet Sensory Innervation Ismentioning

confidence: 99%

Parasympathetic Innervation and Function of Endocrine Pancreas Requires the Glial Cell Line–Derived Factor Family Receptor α2 (GFRα2)

Rossi

Santamäki²,

Airaksinen³

et al. 2005

Diabetes

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Vagal parasympathetic input to the islets of Langerhans is a regulator of islet hormone secretion, but factors promoting parasympathetic islet innervation are unknown. Neurturin signaling via glial cell line-derived neurotrophic factor family receptor ␣2 (GFR␣2) has been demonstrated to be essential for the development of subsets of parasympathetic and enteric neurons. Here, we show that the parasympathetic nerve fibers and glial cells within and around the islets express GFR␣2 and that islet parasympathetic innervation in GFR␣2 knockout (KO) mice is reduced profoundly. In wild-type mice, neuroglucopenic stress produced a robust increase in plasma levels of islet hormones. In the GFR␣2-KO mice, however, pancreatic polypeptide and insulin responses were completely lost and glucagon response was markedly impaired. Islet morphology and sympathetic innervation, as well as basal secretions of the islet hormones, were unaffected. Moreover, a glucose tolerance test failed to reveal differences between the genotypes, indicating that direct glucose-stimulated insulin secretion was not affected by GFR␣2 deficiency. These results show that GFR␣2 signaling is needed for development of the parasympathetic islet innervation that is critical for vagally induced hormone secretion. The GFR␣2-KO mouse represents a useful model to study the role of parasympathetic innervation of the endocrine pancreas in glucose homeostasis. Diabetes 54:1324 -1330, 2005 E ndocrine cells in the islets of Langerhans are well innervated by sympathetic, parasympathetic, and sensory nerve fibers. The parasympathetic branch is thought to be a regulator of the physiological islet hormone secretion (1-4). The parasympathetic fibers in the endocrine pancreas originate from neurons in the intrapancreatic ganglia (5) that receive preganglionic input from the brainstem via the vagus nerve, as well as direct input from enteric (6) and other intrapancreatic neurons (7). Activation of the vagus nerve is known to stimulate the secretion of insulin and other pancreatic hormones, although the relative contributions of noncholinergic parasympathetic neurotransmitters and the enteropancreatic projection to islet hormone secretion remain elusive (3). Meal-induced insulin secretion is traditionally divided into a preabsorptive or cephalic phase that is vagally mediated (3,4) and a subsequent and much larger postabsorptive or postprandial phase that is thought to be mainly controlled by circulating glucose levels. However, several studies (8 -10) suggest that parasympathetic regulation of postprandial insulin secretion may be more important than previously believed.Sympathetic innervation of the pancreatic islets is promoted by the nerve growth factor (11,12). Factors that control the development and maintenance of islet parasympathetic innervation, by contrast, are poorly known. Neurturin, a member of the glial cell line-derived factor family, signals through glial cell line-derived factor family receptor ␣2 (GFR␣2) and has been found to be essential for the developmen...

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The plant lectin Bandeiraea simplicifolia I-B4 identifies a subpopulation of small diameter primary sensory neurones which innervate the skin in the rat

Cited by 84 publications

References 14 publications

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Intense Isolectin-B4 Binding in Rat Dorsal Root Ganglion Neurons Distinguishes C-Fiber Nociceptors with Broad Action Potentials and High Nav1.9 Expression

Parasympathetic Innervation and Function of Endocrine Pancreas Requires the Glial Cell Line–Derived Factor Family Receptor α2 (GFRα2)

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The plant lectin Bandeiraea simplicifolia I-B4 identifies a subpopulation of small diameter primary sensory neurones which innervate the skin in the rat

Cited by 84 publications

References 14 publications

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B4 on primary sensory neurones in seven mammalian species

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B4 on primary sensory neurones in seven mammalian species

Intense Isolectin-B4 Binding in Rat Dorsal Root Ganglion Neurons Distinguishes C-Fiber Nociceptors with Broad Action Potentials and High Nav1.9 Expression

Parasympathetic Innervation and Function of Endocrine Pancreas Requires the Glial Cell Line–Derived Factor Family Receptor α2 (GFRα2)

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Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species

Analysis of the distribution of binding sites for the plant lectin Bandeiraea simplicifolia I‐isolectin B₄ on primary sensory neurones in seven mammalian species