The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein

Bodner, Mordechai; Castriilo, José-Luis; Theill, Lars E.; Deerinck, Thomas J.; Ellisman, Mark H.; Karin, Michael

doi:10.1016/0092-8674(88)90037-2

Cited by 752 publications

(347 citation statements)

References 52 publications

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“…After transfer, the protein containing membrane was blocked using ECL Advance Blocking Agent (ECL Advance Western Blotting Detection kit, Amersham Biosciences) and reacted during 1 h with an anti-rat POU1F1 primary antibody diluted 1:500. This antibody was used in the studies of bovine (Bodner et al, 1988), human (Vila et al, 1993) and pig (Malagon et al, 1996) POU1F1. This first incubation was followed by a 1 h incubation with donkey anti-rabbit coupled to horseradish peroxidase secondary antibody (Amersham Biosciences) diluted 1:50,000.…”

Section: Protein Electrophoresis and Western Blot Analysismentioning

confidence: 99%

“…POU1F1 (also named PIT-1 or GHF-1) is a member of the POU homeodomain family of transcription factors (Bodner et al, 1988;Ingraham et al, 1988). It is mainly expressed in the pituitary and its expression is necessary for the normal differentiation, development and survival of three adenohypophysis cell types, thyrotrophs, somatotrophs and lactotrophs (Li et al, 1990;Simmons et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of four splicing variants of ovine POU1F1 gene

Bastos

Ávila

Cravador

et al. 2006

Gene

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Expression of POU1F1 gene, a member of the POU homeodomain family of transcription factors, is necessary for normal differentiation, development and survival of three anterior pituitary cell types (thyrotrophs, somatotrophs and lactotrophs) and for the proper expression of growth hormone (GH ), prolactin (PRL), thyroid-stimulating hormone (TSH ) genes and POU1F1 gene itself. Alternative splicing forms of this gene have been reported in different species, with few functional studies. Apart from the POU1F1-Wild-type with the expected length, in this work we isolated three additional splicing variants: POU1F1-β, with a 78 bp insert in the trans-activation domain; POU1F1-γ that lacks exon 3 and POU1F1-δ that lacks exons 3, 4 and 5. Four different protein isoforms were also detected by Western blot in the sheep pituitary tissue. Functional assays were performed to study the trans-activation of GH and PRL promoters by the splicing variants. Regarding the PRL promoter, the β variant presented only 12% of the Wild-type trans-activation capacity. Variants γ and δ showed no capacity to trans-activate PRL promoter. Both γ and δ variants acted as repressors of Wt, reducing significantly the trans-activation made by Wt alone ( p < 0.05). Concerning the GH promoter, the β variant presented a trans-activation capacity 10% higher than Wt. Wt and β variants strongly interact in the activation of GH promoter doubling the trans-activation potential of Wt. Variants γ and δ showed no capacity to trans-activate the GH promoter and both acted as repressors, reducing significantly ( p < 0.001) the trans-activation performed by Wt. This work presents, for the first time, the characterization of four splicing forms of Ovis aries POU1F1 gene.

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Section: Protein Electrophoresis and Western Blot Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and characterization of four splicing variants of ovine POU1F1 gene

Bastos

Ávila

Cravador

et al. 2006

Gene

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“…As a key to answer this question, considerable attention has been paid to transcription factors that are specific to the pituitary gland. In 1988, Bodner et al (28) and Ingraham et al (29) separately reported the presence of a pituitary specific transcription factor (Pit-1/GHF-1).…”

Section: Discussionmentioning

confidence: 99%

Expression of Pituitary Homeo Box 1 (Ptx1) in Human Non-Neoplastic Pituitaries and Pituitary Adenomas

et al. 2000

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“…In other homeobox-containing proteins, the homeodomain appears to be sutiicient for DNA binding. Two other proteins, unc-86 and Pit-1 or growth hormone factor-I, also contain the POU domain [26][27][28]. The function of unc-86 is in development in Caenorbabditis ele, gans to regulate neuronal cell differentiation, while Pit-1 is a rat-pituitary-specific transcriptional factor, regulating transcription in lactotrophic and somatotrophic cell types.…”

Section: Homeoboxes Are Transcriptional Factors In Vertebratesmentioning

confidence: 99%

The homeobox in vertebrate development

Goulding

Gruß

1989

Current Opinion in Cell Biology

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The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein

Cited by 752 publications

References 52 publications

Identification and characterization of four splicing variants of ovine POU1F1 gene

Identification and characterization of four splicing variants of ovine POU1F1 gene

Expression of Pituitary Homeo Box 1 (Ptx1) in Human Non-Neoplastic Pituitaries and Pituitary Adenomas

The homeobox in vertebrate development

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