Expression of Pituitary Homeo Box 1 (Ptx1) in Human Non-Neoplastic Pituitaries and Pituitary Adenomas

Tahara, Shigeyuki; Kurotani, Reiko; Sanno, Naoko; Takumi, Ichiro; Yoshimura, Satoshi; Osamura, R. Yoshiyuki; Teramoto, Akira

doi:10.1038/modpathol.3880204

Cited by 13 publications

(4 citation statements)

References 38 publications

(30 reference statements)

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“…Interestingly, most of the multi-hormonal cells in our scRNA-seq data express lhb / fshb , lhb / tshba or fshb / tshba . In mammals, differentiation of progenitor cells to both gonadotropes and thyrotropes requires the transcription factor Gata2 ( 86 , 87 ), but co-expression with Sf1 leads to gonadotropes whereas co-expression with Pit1 leads to thyrotropes ( 88 – 91 ). In contrast, relatively little is known regarding pituitary cell lineage in teleosts.…”

Section: Discussionmentioning

confidence: 99%

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

et al. 2021

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In vertebrates, the anterior pituitary plays a crucial role in regulating several essential physiological processes via the secretion of at least seven peptide hormones by different endocrine cell types. Comparative and comprehensive knowledge of the spatial distribution of those endocrine cell types is required to better understand their physiological functions. Using medaka as a model and several combinations of multi-color fluorescence in situ hybridization, we present the first 3D atlas revealing the gland-wide distribution of seven endocrine cell populations: lactotropes, thyrotropes, Lh and Fsh gonadotropes, somatotropes, and pomca-expressing cells (corticotropes and melanotropes) in the anterior pituitary of a teleost fish. By combining in situ hybridization and immunofluorescence techniques, we deciphered the location of corticotropes and melanotropes within the pomca-expressing cell population. The 3D localization approach reveals sexual dimorphism of tshba-, pomca-, and lhb-expressing cells in the adult medaka pituitary. Finally, we show the existence of bi-hormonal cells co-expressing lhb-fshb, fshb-tshba and lhb-sl using single-cell transcriptomics analysis and in situ hybridization. This study offers a solid basis for future comparative studies of the teleost pituitary and its functional plasticity.

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Section: Discussionmentioning

confidence: 99%

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

et al. 2021

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“…They are T-pit, RPx/Hesx-1, Ptx1, Ptx2, Lhx3/P-lim, Prop-1, Pit-1, SF-1, NeuroD-1, GATA-2 [16, 17]. For example, the GATA-2 is responsible for differentiation of gonadotropes and thyrotropes.…”

Section: Discussionmentioning

confidence: 99%

Plurihormonal cells of normal anterior pituitary: Facts and conclusions

et al. 2017

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Introductionplurihormonality of pituitary adenomas is an ability of adenoma cells to produce more than one hormone. After the immunohistochemical analysis had become a routine part of the morphological study, a great number of adenomas appeared to be multihormonal in actual practice. We hypothesize that the same cells of a normal pituitary gland releases several hormones simultaneously.ObjectiveTo analyse a possible co-expression of hormones by the cells of the normal anterior pituitary of adult humans in autopsy material.Materials and methodsWe studied 10 pituitary glands of 4 women and 6 men with cardiovascular and oncological diseases. Double staining immunohistochemistry using 11 hormone combinations was performed in all the cases. These combinations were: prolactin/thyroid-stimulating hormone (TSH), prolactin/luteinizing hormone (LH), prolactin/follicle-stimulating hormone (FSH), prolactin/adrenocorticotropic hormone (ACTH), growth hormone (GH)/TSH, GH/LH, GH/FSH, GH/ACTH, TSH/LH, TSH/FSH, TSH/ACTH. Laser Confocal Scanning Microscopy with a mixture of primary antibodies was performed in 2 cases. These mixtures were ACTH/prolactin, FSH/prolactin, TSH/prolactin, ACTH/GH, and FSH/GH.ResultsWe found that the same cells of the normal adenohypophysis can co-express prolactin with ACTH, TSH, FSH, LH; GH with ACTH, TSH, FSH, LH, and TSH with ACTH, FSH, LH. The comparison of the average co-expression coefficients of prolactin, GH and TSH with other hormones showed that the TSH co-expression coefficient was significantly the least (9,5±6,9%; 9,6±7,8%; 1,0±1,3% correspondingly).ConclusionPlurihormonality of normal adenohypophysis is an actually existing phenomenon. Identification of different hormones in pituitary adenomas enables to find new ways to improve both diagnostic process and targeted treatment.

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“…In addition, various transcription factors play specific roles in the development and differentiation of cells in the anterior pituitary. Conversely, as similar transcription factors are expressed in both pituitary adenoma and normal hormone-producing cells, normal hormone-producing cells in the pituitary that have matured and functionally differentiated may abnormally proliferate in a monoclonal manner and, eventually, develop into pituitary adenoma [6][7][8][9]. However, the precise mechanisms underlying the development of pituitary adenoma have yet to be clarified.…”

Section: Introductionmentioning

confidence: 93%

Expression of MSX1 in Human Normal Pituitaries and Pituitary Adenomas

et al. 2008

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Transcription factors play specific roles in the development and differentiation of normal pituitary tissues and pituitary adenoma. The transcription factor, muscle segment homeobox 1 (MSX1), which belongs to the homeobox gene family, binds the promoter region of the glycoprotein hormone alpha-subunit (SU) in TSH-producing cells in the mouse pituitary and regulates alpha-SU expression. The present study investigated MSX1 expression in the normal human pituitary. In addition, 50 pituitary adenomas were examined using immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) to clarify the role of MSX1 in the development and functional differentiation of pituitary adenoma cells. In the normal pituitary, MSX1 was predominantly expressed in the cytoplasm of GH-producing cells. Furthermore, MSX1 immunoreactivity was observed in the cytoplasm of some alpha-SU-producing cells. It is interesting to note that, in the pituitary adenoma, MSX1 was expressed in the nucleus of GH- and TSH-producing adenomas. RT-PCR using RNA extracted and purified from formalin-fixed paraffin-embedded pituitary adenoma specimens revealed MSX1 mRNA expressed in GH- and TSH-producing adenomas. Immunoelectron microscopy demonstrated MSX1 localized at intranuclear heterochromatin and euchromatin, which suggests transcriptional activity. These results suggest that MSX1 plays a specific role in human pituitary adenoma.

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Expression of Pituitary Homeo Box 1 (Ptx1) in Human Non-Neoplastic Pituitaries and Pituitary Adenomas

Cited by 13 publications

References 38 publications

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

Plurihormonal cells of normal anterior pituitary: Facts and conclusions

Expression of MSX1 in Human Normal Pituitaries and Pituitary Adenomas

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