The Pilot Experience of Immunotherapy-Combined Photodynamic Therapy for Advanced Gastric Cancer in Elderly Patients

Yanai, Hideo; Kuroiwa, Yasuyuki; Shimizu, Norio; Matsubara, Yoshitaka; Okamoto, Takeshi; Hirano, Atsuyoshi; Nakamura, Youhei; Okita, Kiwamu; Sekine, Teruaki

doi:10.1385/ijgc:32:2-3:139

Cited by 13 publications

(4 citation statements)

References 2 publications

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“…PDT, a non- or minimally invasive therapeutic approach, is based on tumor-specific accumulation of a photosensitizer followed by its activation using an appropriate wavelength of laser light, which ultimately causes the generation of highly ROS within the tumor cells and induces tumor cell death [ 8 , 14 , 22 , 23 ]. PDT is almost pain-free and more convenient for both patients and clinics and has been applied to gastrointestinal tumors [ 24 – 26 ], including colon and rectal cancers. Because PDT does not cause essential effect on connective tissues, the anatomical integrity of hollow organs such as the colon can be preserved in patients undergoing PDT [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials

Abdulrehman

et al. 2018

Lasers Med Sci

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The aim of this study is to investigate the antitumor effects and possible mechanisms of meta-tetrahydroxyphenylchlorin-mediated photodynamic therapy (m-THPC-PDT) on human primary (SW480) and metastatic (SW620) colon cancer cell lines. SW480 and SW620 cells were incubated with various concentrations of m-THPC, followed by photodynamic irradiation. Subcellular localization of m-THPC in cells was observed with confocal laser scanning microscopy (CLSM). Photocytotoxicity of m-THPC in the two cells was investigated by using MTT assay. The flow cytometry was employed to detect the cell apoptosis. The migration and long-term recovery ability were determined by scratch test and colony formation assay respectively. CLSM showed that m-THPC was mainly distributed within the endoplasmic reticulum (ER) and lysosome of SW480 cells and within the lysosome and mitochondria of SW620 cells. m-THPC-PDT induced a dose-dependent and light energy-dependent cytotoxicity in SW480 and SW620 cells. Apoptosis rate was approximately 65 and 25% in SW480 and SW620 respectively when the concentration of m-THPC increased to 11.76 μM. However, the rate of necrotic cells had no significant changes in two cell lines. The colony formation and migration ability of the two cell lines were decreased with m-THPC-PDT treatment in a dose-dependent manner. PDT with m-THPC not only could effectively inhibit cell proliferation and decrease migration ability and colony formation ability, but also could effectively kill SW480 and SW620 cells in a dose-dependent manner in vitro. These results suggest that m-THPC is a promising sensitizer that warrants further development and extensive studies towards clinical use of colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials

Abdulrehman

et al. 2018

Lasers Med Sci

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“…169 Early clinical studies from Japan of PDT in the stomach suggested great promise 170-171 , but regrettably were not followed by randomized clinical trials so far. PDT for early duodenal and ampullary cancers and advanced adenomas has been also investigated in pilot studies that indicated promising results, but further work is required to optimize the treatment conditions.…”

Section: Clinical Pdtmentioning

confidence: 99%

Photodynamic therapy of cancer: An update

Agostinis

Berg

Cengel

et al. 2011

CA: A Cancer Journal for Clinicians

4,243

3,792

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Photodynamic therapy (PDT) is a clinically approved, minimally invasive therapeutic procedure that can exert a selective cytotoxic activity toward malignant cells. The procedure involves administration of a photosensitizing agent followed by irradiation at a wavelength corresponding to an absorbance band of the sensitizer. In the presence of oxygen, a series of events lead to direct tumor cell death, damage to the microvasculature and induction of a local inflammatory reaction. Clinical studies revealed that PDT can be curative particularly in early-stage tumors. It can prolong survival in inoperable cancers and significantly improve quality of life. Minimal normal tissue toxicity, negligible systemic effects, greatly reduced long-term morbidity, lack of intrinsic or acquired resistance mechanisms, and excellent cosmetic as well as organ function-sparing effects of this treatment make it a valuable therapeutic option for combination treatments. With a number of recent technological improvements, PDT has the potential to become integrated into the mainstream of cancer treatment.

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“…The U.S. FDA approved photofrin-mediated PDT for patients with Barret's esophagus and high grade dysplasia who did not undergo surgery [34] . PDT has been applied to other GI digestival tumors under the stomach [35,36] , cholangiocarcinoma (CG) [37] , with a therapeutic response on unresectable pancreatic cancers [38] , and on colon or rectal cancers [39,40] .…”

Section: Clinical Applications Of Pdt In a Variety Of Human Cancersmentioning

confidence: 99%

Dual roles of nitric oxide in the regulation of tumor cell response and resistance to photodynamic therapy

2015

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Photodynamic therapy (PDT) against cancer has gained attention due to the successful outcome in some cancers, particularly those on the skin. However, there have been limitations to PDT applications in deep cancers and, occasionally, PDT treatment resulted in tumor recurrence. A better understanding of the underlying molecular mechanisms of PDT-induced cytotoxicity and cytoprotection should facilitate the development of better approaches to inhibit the cytoprotective effects and also augment PDT-mediated cytotoxicity. PDT treatment results in the induction of iNOS/NO in both the tumor and the microenvironment. The role of NO in cytotoxicity and cytoprotection was examined. The findings revealed that NO mediates its effects by interfering with a dysregulated pro-survival/anti-apoptotic NF-κB/Snail/YY1/RKIP loop which is often expressed in cancer cells. The cytoprotective effect of PDT-induced NO was the result of low levels of NO that activates the pro-survival/anti-apoptotic NF-κB, Snail, and YY1 and inhibits the anti-survival/pro-apoptotic and metastasis suppressor RKIP. In contrast, PDT-induced high levels of NO result in the inhibition of NF-kB, Snail, and YY1 and the induction of RKIP, all of which result in significant anti-tumor cytotoxicity. The direct role of PDT-induced NO effects was corroborated by the use of the NO inhibitor, l-NAME, which reversed the PDT-mediated cytotoxic and cytoprotective effects. In addition, the combination of the NO donor, DETANONOate, and PDT potentiated the PDT-mediated cytotoxic effects. These findings revealed a new mechanism of PDT-induced NO effects and suggested the potential therapeutic application of the combination of NO donors/iNOS inducers and PDT in the treatment of various cancers. In addition, the study suggested that the combination of PDT with subtoxic cytotoxic drugs will result in significant synergy since NO has been shown to be a significant chemo-immunosensitizing agent to apoptosis.

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The Pilot Experience of Immunotherapy-Combined Photodynamic Therapy for Advanced Gastric Cancer in Elderly Patients

Abstract: I-PDT was found to be a safe, feasible treatment that could improve symptoms resulting from advanced gastric cancer.

Cited by 13 publications

References 2 publications

Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials

Effects of meta-tetrahydroxyphenylchlorin photodynamic therapy on isogenic colorectal cancer SW480 and SW620 cells with different metastatic potentials

Photodynamic therapy of cancer: An update

Dual roles of nitric oxide in the regulation of tumor cell response and resistance to photodynamic therapy

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