The physiology of trace elements in biological methane production

Azim, Annalisa Abdel; Pruckner, Christian; Kolar, Philipp; Taubner, Ruth-Sophie; Fino, Debora; Saracco, Guido; Sousa, Filipa L.; Rittmann, Simon K.-M. R.

doi:10.1016/j.biortech.2017.05.211

Cited by 30 publications

(25 citation statements)

References 40 publications

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“…First, M. marburgensis was grown at 65 °C and a pH of 7.0 with exponential feeding of gas and sulfur (Abdel Azim et al 2017). Second, M. thermaggregans was grown at 60 °C and a pH of 7.0 with exponential gas and sulfur feeding.…”

Section: Resultsmentioning

confidence: 99%

“…As iron-sulfur clusters are abundant in many enzyme complexes in the Wood-Ljungdahl pathway, most methanogens require external sulfur sources for the synthesis of these complexes (Abdel Azim et al 2017; Thauer 1990; Thauer et al 2008). As a side effect, DS also leads to the reduction of chemical compounds in the medium.…”

Section: Discussionmentioning

confidence: 99%

“…The inoculum for all fed-batch cultivations of M. thermaggregans and of M. marburgensis was obtained from fed-batch cultivations. Harvesting of high cell density biomass was done by using methods previously described (Abdel Azim et al 2017). All fed-batch cultivations of M. thermaggregans and of M. marburgensis were performed using M. marburgensis medium (MM) (Rittmann et al 2012).…”

Section: Methodsmentioning

confidence: 99%

“…H 2 and CO 2 uptake rates and MER were calculated by determining the off-gas composition and calculating or measuring the off-gas volumetric flow rate. The off-gas composition (H 2 , CO 2 , and CH 4 ) during cultivation was analyzed via gas chromatography (7890A GC System, Aligent Technologies, Santa Clara, USA) by using a TCD detector and a 19808 Shin Carbon ST Micropacked Column (Restek GmbH, Bad Homburg, Germany) as described before (Taubner and Rittmann 2016; Abdel Azim et al 2017). Automated sampling of serum bottle headspace and subsequent gas injection into the gas chromatograph for off-gas analysis was accomplished by using a gas injection and control unit (Joint Analytical Systems GmbH, Moers, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Liquid-limited conditions are encountered by an organism if, e.g., trace elements are limiting the growth and/or gas production. However, before a methanogen is investigated in continuous culture mode, it is highly beneficial to utilize closed batch or fed-batch CO 2 -BMP systems for the initial examination of the physiological and biotechnological characteristics of the organism (Abdel Azim et al 2017; Taubner and Rittmann 2016). …”

Section: Introductionmentioning

confidence: 99%

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Physiology and methane productivity of Methanobacterium thermaggregans

Mauerhofer

Reischl

Schmider

et al. 2018

Appl Microbiol Biotechnol

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Accumulation of carbon dioxide (CO2), associated with global temperature rise, and drastically decreasing fossil fuels necessitate the development of improved renewable and sustainable energy production processes. A possible route for CO2 recycling is to employ autotrophic and hydrogenotrophic methanogens for CO2-based biological methane (CH4) production (CO2-BMP). In this study, the physiology and productivity of Methanobacterium thermaggregans was investigated in fed-batch cultivation mode. It is shown that M. thermaggregans can be reproducibly adapted to high agitation speeds for an improved CH4 productivity. Moreover, inoculum size, sulfide feeding, pH, and temperature were optimized. Optimization of growth and CH4 productivity revealed that M. thermaggregans is a slightly alkaliphilic and thermophilic methanogen. Hitherto, it was only possible to grow seven autotrophic, hydrogenotrophic methanogenic strains in fed-batch cultivation mode. Here, we show that after a series of optimization and growth improvement attempts another methanogen, M. thermaggregas could be adapted to be grown in fed-batch cultivation mode to cell densities of up to 1.56 g L−1. Moreover, the CH4 evolution rate (MER) of M. thermaggregans was compared to Methanothermobacter marburgensis, the CO2-BMP model organism. Under optimized cultivation conditions, a maximum MER of 96.1 ± 10.9 mmol L−1 h−1 was obtained with M. thermaggregans—97% of the maximum MER that was obtained utilizing M. marburgensis in a reference experiment. Therefore, M. thermaggregans can be regarded as a CH4 cell factory highly suited to be applicable for CO2-BMP.Electronic supplementary materialThe online version of this article (10.1007/s00253-018-9183-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Physiology and methane productivity of Methanobacterium thermaggregans

Mauerhofer

Reischl

Schmider

et al. 2018

Appl Microbiol Biotechnol

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Thiol‐Disulfide Exchange Kinetics and Redox Potential of the Coenzyme M and Coenzyme B Heterodisulfide, an Electron Acceptor Coupled to Energy Conservation in Methanogenic Archaea

Laird,

Adlung,

Koivisto

et al. 2023

ChemBioChem

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Methanogenic and methanotrophic archaea play important roles in the global carbon cycle by interconverting CO2 and methane. To conserve energy from these metabolic pathways that happen close to the thermodynamic equilibrium, specific electron carriers have evolved to balance the redox potentials between key steps. Reduced ferredoxins required to activate CO2 are provided by energetical coupling to the reduction of the high‐potential heterodisulfide (HDS) of coenzyme M (2‐mercaptoethanesulfonate) and coenzyme B (7‐mercaptoheptanoylthreonine phosphate). While the standard redox potential of this important HDS has been determined previously to be −143 mV (Tietze et al. 2003 DOI: 10.1002/cbic.200390053), we have measured thiol disulfide exchange kinetics and reassessed this value by equilibrating thiol‐disulfide mixtures of coenzyme M, coenzyme B, and mercaptoethanol. We determined the redox potential of the HDS of coenzyme M and coenzyme B to be −16.4±1.7 mV relative to the reference thiol mercaptoethanol (E0’=−264 mV). The resulting E0’ values are −281 mV for the HDS, −271 mV for the homodisulfide of coenzyme M, and −270 mV for the homodisulfide of coenzyme B. We discuss the importance of these updated values for the physiology of methanogenic and methanotrophic archaea and their implications in terms of energy conservation.

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Biomass Digestion for Production of Electrical Energy

Sharma¹,

Gupta²

2022

Clean Energy Production Technologies

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The physiology of trace elements in biological methane production

Cited by 30 publications

References 40 publications

Physiology and methane productivity of Methanobacterium thermaggregans

Physiology and methane productivity of Methanobacterium thermaggregans

Thiol‐Disulfide Exchange Kinetics and Redox Potential of the Coenzyme M and Coenzyme B Heterodisulfide, an Electron Acceptor Coupled to Energy Conservation in Methanogenic Archaea

Biomass Digestion for Production of Electrical Energy

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