The Physiology of Secretin

Grossman, Morton I.

doi:10.1016/s0083-6729(08)60317-9

Cited by 21 publications

(5 citation statements)

References 120 publications

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“…These changes, and the changes in the concentration of chloride and bicarbonate with pancreatic secretion rate seen in calf 3/3, agree with those reported by McCormick & Stcwart (1967) and are entirely consistent vtrith the known actions and pattern of secretion of secretin as has been found for many species (Bayliss & Starling, 1902; Grossman, 1958). Taylor, 1962).…”

Section: Discussionsupporting

confidence: 90%

Digestion and absorption of dry and high-moisture maize diets in the small and large intestine of the pig

Holmes¹,

Bayley²,

Horney³

1973

BJN

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I. Two surgical techniques were developed in the calf to study the role of pancreatic secretion in digestion. The secretion was collected through a cannula placed in a small sac of duodenum into which the pancreatic duct drained. The continuity of the duodenum was re-established in the first technique by duodenal reentrant cannulas, and in the second technique by end-to-end anastomosis of the duodenum with a cannula placed cranial to the anastomosis to return the pancreatic secretion. The accessory pancrcatic duct was ligated.z. The flows of digesta through the duodenum of milk-fed calves were 5505, 6369 and 7709 ml/rz h at 7, 24 and 63 d of age respectively, similar to values reported previously in the literature. In a 12 h collection period 297, 441 and 602 ml pancrcatic fluid werc sccreted by calves of 7,24 and 63 d of age respectively. The secretion from the mucosa of the duodenal sac was 40 ml/12 h in t w o other calves.3. The rate of secretion from the pancreas varied markedly in milk-fed calves, being lowest 2-3 h and highest 6-10 h after feeding. Changes in the concentration of chloride and bicarbonate with pancreatic secretion rate were indicative of a secretin stimulus to secretion.4. The rates of inactivation of pancreatic enzymes collected from the duodenal sac were measured at 4O, zon and 39O.

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Section: Discussionsupporting

confidence: 90%

Digestion and absorption of dry and high-moisture maize diets in the small and large intestine of the pig

Holmes¹,

Bayley²,

Horney³

1973

BJN

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“…There are a variety of stimuli that can "polarize" the secretory cells of the pancreas in the manner of secretin; all appear to produce an increase in HC03-concentration and flow (201). Secretin may be taken as representative since it is a natural mech- (192). The change in [HCOa-] from resting to stimulated secretion is from about 40 to 130 m.~ (201).…”

Section: F Pancreasmentioning

confidence: 99%

Carbonic anhydrase: chemistry, physiology, and inhibition

Th¹

1967

Physiological Reviews

1,804

841

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The recent review on this subject by Davis (117) makes it possible to reduce this section to a brief critical outline of the types of methods used for determination of carbonic anhydrase and its inhibitors. A few papers published since Davis' review are mentioned individually. All methods depend on either the evolution or absorption of CO2 or on a pH change. It is obvious that a wide variety of physical techniques and chemical principles is applicable. A. Manometric methods These were developed by Meldrum and Roughton (350) at the time they discovered the enzyme and were refined in later years by Roughton and colleagues. The basic reaction is evolution of CO2 from NaHCOs-buffer mixtures or uptake of CO2 by buffer solutions. A bibliography and description are given in reference 117. These methods have yielded a great deal of useful information. In recent years, however, their use has lessened, for at least three reasons: rapid and vigorous shaking has frequently damaged the enzyme; the methods are somewhat cumbersome; and they are not suitable for rapid flow measurements. An inherent advantage of the manometric principle, however, is its potential for extreme sensitivity. Using the Cartesian diver, it has been possible to detect carbonic anhydrase in single red cells, glia, and epithelia of choroid plexus (17 1). B. pH methods T. H. MAREN Vohme 47 activity, it must be shown that the procedure is run more rapidly than the uncatalyzed rate. For the dehydration reaction, the pH is critical in calculating this rate. The implication in Hausler's (203) and Hansson's (198) methods is that at the surface and at the critical areas of the section, evolution of CO2 markedly elevates the pH, slowing the uncatalyzed rate. .This pH is not known, but if it were 7.4, the half-time for dehydration is 3 min; if pH were 8.4, it is 30 min. In the Hansson method (198) sections float for only 2-10 min, so that it is reasonable to suppose that enzyme activity is involved. Hansson (198) was also the first to show inhibition of the reaction at low (0.3 PM) concentrations of acetazolamide, ruling out complexation of cobalt present (1.3 mu). The drug at 20 mg/kg given 30 min before autopsy also inhibited staining. It is anticipated that the application of this new method will permit recognition of carbonic anhydrase in specific secretory sites, as well as in places (i.e. skin) where in the chemical method the mass of tissue may obscure enzyme in a relatively few critical cells. The nature of the cobalt deposit in the incubated tissue sections is at present unknown. Hansson (198), using a phosphate-bicarbonate medium quite different from that of Hausler, studied the uptake of 6oCo and 32P into sections during incubation and found the tissue content of both isotopes increased. From this he concluded that the deposit was a basic cobalt phosphate salt. By analogy, the deposit in Hausler's method may be a cobalt bicarbonate complex. Hansson (197) has demonstrated the enzyme in red cells by fluorescent antibodies; it would be interesting if this ...

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“…Tyr-Ala -Glu-Gly-Thr-Phe-Ile -Ser -Asp-Tyr-Ser -le -Ala-Met-15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 VIP Lys-Gln -Met-Ala -Val -Lys-Lys -Tyr-Leu-Asn-Ser -Je -Leu-Asn-NH2 Secretin Asp-Ser -Ala -Arg-Leu-Gln -Arg-Leu-Leu-Gln-Gly -Leu-Val -NH2 Glucagon Asp-Ser -Arg-Arg-Ala -Gln -Asp-Phe-Val -Gln -Trp-Leu-Met-Asp-Thr GIP Asp-Lys-Ile -Arg-Gln-Asp-Phe-Val-Asn-Trp-Leu-Ala-Gln-Gln extensively studied and its importance for the stimulation of pancreatic water and bicarbonate and thereby the regulation of duodenal acidity, was established (Grossman, 1958). However, secretin itself successfully eluded its many pursuers; early hopes that it might prove to be a simple base like adrenaline, which had been isolated and identified chemically by Takamine (1901) were soon dashed.…”

Section: Gipmentioning

confidence: 99%

The Bayliss‐Starling lecture 1973. The gastrointestinal hormones: a review of recent advances.

Gregory¹

1974

The Journal of Physiology

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The Physiology of Secretin

Cited by 21 publications

References 120 publications

Digestion and absorption of dry and high-moisture maize diets in the small and large intestine of the pig

Digestion and absorption of dry and high-moisture maize diets in the small and large intestine of the pig

Carbonic anhydrase: chemistry, physiology, and inhibition

The Bayliss‐Starling lecture 1973. The gastrointestinal hormones: a review of recent advances.

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