The physicochemical and mechanical investigation of siloxane modified Gelatin/Sodium alginate injectable hydrogels loaded by ascorbic acid and β-Glycerophosphate

Rezaei, Hessam; Asefnejad, Azadeh; Joupari, Morteza Daliri; Joughehdoust, Sedigheh

doi:10.1016/j.mtcomm.2020.101914

Cited by 9 publications

(7 citation statements)

References 61 publications

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“…In additional, the presence of amide and carboxyl groups in the structure of gelatin increases the hybridization process in a liquid environment. Water molecules can penetrate freely into the gelatin-added material and damage its structure, a study by Rezaei et al [ 85 ] so confirms.…”

Section: Resultsmentioning

confidence: 89%

Changes in the Mechanical Properties of Alginate-Gelatin Hydrogels with the Addition of Pygeum africanum with Potential Application in Urology

Kurowiak

Kaczmarek-Pawelska

Mackiewicz

et al. 2022

IJMS

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New hydrogel materials developed to improve soft tissue healing are an alternative for medical applications, such as tissue regeneration or enhancing the biotolerance effect in the tissue-implant–body fluid system. The biggest advantages of hydrogel materials are the presence of a large amount of water and a polymeric structure that corresponds to the extracellular matrix, which allows to create healing conditions similar to physiological ones. The present work deals with the change in mechanical properties of sodium alginate mixed with gelatin containing Pygeum africanum. The work primarily concentrates on the evaluation of the mechanical properties of the hydrogel materials produced by the sol–gel method. The antimicrobial activity of the hydrogels was investigated based on the population growth dynamics of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923, as well as the degree of degradation after contact with urine using an innovative method with a urine flow simulation stand. On the basis of mechanical tests, it was found that sodium alginate-based hydrogels with gelatin showed weaker mechanical properties than without the additive. In addition, gelatin accelerates the degradation process of the produced hydrogel materials. Antimicrobial studies have shown that the presence of African plum bark extract in the hydrogel enhances the inhibitory effect on Gram-positive and Gram-negative bacteria. The research topic was considered due to the increased demand from patients for medical devices to promote healing of urethral epithelial injuries in order to prevent the formation of urethral strictures.

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Section: Resultsmentioning

confidence: 89%

Changes in the Mechanical Properties of Alginate-Gelatin Hydrogels with the Addition of Pygeum africanum with Potential Application in Urology

Kurowiak

Kaczmarek-Pawelska

Mackiewicz

et al. 2022

IJMS

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“…64 The RGD sequences, that is, glycine, proline, and hydroxyproline amino acids, of G provide high cell interaction. 65 As suggested before, TiO 2 with a low diameter (<30 nm) can enhance cell adhesion and growth due to its highly curved nanotube edges, providing optimum length for integrin clustering and focal contact formation. 66 However, there is a concern about the cytotoxicity effect of TiO 2 due to the cellular membrane interactions.…”

Section: Scratch Assaymentioning

confidence: 82%

“…Although polycaprolactone can decrease cell behavior of the samples, the use of natural Gel, silk fibroin, and TiO 2 can be overcome on polycaprolactone limitation 64 . The RGD sequences, that is, glycine, proline, and hydroxyproline amino acids, of G provide high cell interaction 65 . As suggested before, TiO 2 with a low diameter (<30 nm) can enhance cell adhesion and growth due to its highly curved nanotube edges, providing optimum length for integrin clustering and focal contact formation 66 .…”

Section: Resultsmentioning

confidence: 99%

Investigation of co‐electrospun gelatin:TiO₂/polycaprolactone:silk fibroin scaffolds for wound healing applications

Golipour

Ezzatzadeh

Sadeghianmaryan

2022

J of Applied Polymer Sci

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Electrospinning is a facile technique to create porous nanofiber scaffolds with a high capability of natural environment mimicking. Here, co‐electrospinning of gelatin:TiO2, polycaprolactone:silk fibroin (G:T/P:F) was investigated with the aim of wound healing. Polycaprolactone improved the mechanical properties and gelatin:TiO2 enhanced cellular interactions. Silk fibroin was extracted from the Bombyx mori silkworm and increased the cellular and mechanical properties of the designed scaffolds. The morphology of the scaffolds was observed with field emission scanning electron microscopy (FESEM) and confirmed bead free porous nanofibrous structure. The fiber diameter rose after silk fibroin and TiO2 addition but it was in the favorable fiber range. The contact angle, swelling, and mechanical properties of the G:T/P:F scaffolds supplied the requirements for skin tissue engineering. The designed scaffold showed over 80% cell viability with filopodia formation after 48 h according to cell adhesion observations and MTT assay results. The DAPI and LIVE/DEAD staining results confirmed high cell viability and the scratch assay observations proved the high wound healing potential of the G:T/P:F scaffolds. Furthermore, antibacterial properties were shown. It seems the designed scaffold can be useful in skin tissue regeneration. Further research may address the suggested skin scaffold.

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“…The biodegradation ratio of the prepared scaffolds was calculated using Equation ( 2) after soaking of the scaffolds for 2 weak in phosphatebuffered saline (PBS) at 37 ± 0.5 °C [38]:…”

Section: Biodegradation Ratiomentioning

confidence: 99%

“…Due the presence of hydrophilic groups such as amide and carboxyl in gelatin structures, it hydrolyzed quickly in the presence of water. In addition, other studies indicated that in-vivo gelatin biodegradation was conducted by both hydrolysis and enzymatic manners [38,58]. Besides, the PBS soaking of chitosan can lead to the 1,4 N-acetyl-glucosamine groups hydrolysis and created amino sugars.…”

Section: Biodegradation Ratiomentioning

confidence: 99%

Glutaraldehyde: Introducing Optimum Condition for Cross-linking the Chitosan/Gelatin Scaffolds for Bone Tissue Engineering

Zadeh

Zamanian

2022

IJE

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Large bone defects caused by trauma or disease needs extra intervention. Gelatin/chitosan complex is one of the most valuable compositions for bone healing, but fast degradation in aqueous solution and low mechanical properties increase the need for cross-linking agent. The cross-linker concentration and cross-linking method had a significant effect on the properties of fabricated scaffolds. Here, three different cross-linking methods of Glutaraldehyde (GA), including addition to the solution, vapor exposure, and immersion, were studied by different in-vitro analyses to find the best GA cross-linker concentration and cross-linking method. Scanning electron microscopy showed homogeneous microstructures in all samples. Fourier transform infrared spectrophotometry revealed cross-linking reactions in all samples. Swelling ratio and biodegradation ratio was reduced by increasing cross-linking concentration and exposure time. Nonetheless, higher cross-linker concentration and exposure time improved mechanical properties, while it seems the cross-linking exposure time had more effect than concentration. Accordingly, GA (1 wt%) cross-linked scaffold with solution addition method showed suitable performance with 39.3° contact angle, 1.45±0.05 MPa compressive strength, 22.31±1.3 (%) swelling ratio, and 26.33±4.47 (%) biodegradation ratio. In-vitro experiments indicated cells were spread all over the scaffolds with higher than 80 (%) cell viability in all time points. The expression of alkaline phosphatase (ALP) and osteo-related genes (osteocalcin and related transcription factor 2) were improved during 14 days of cell incubation and showed the high capacity of the scaffold support in mineralization and osto-differentiation. Therefore GA (1 wt% ) cross-linked scaffold with solution addition was introduced as the best candidate for bone repair and further studies.

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The physicochemical and mechanical investigation of siloxane modified Gelatin/Sodium alginate injectable hydrogels loaded by ascorbic acid and β-Glycerophosphate

Cited by 9 publications

References 61 publications

Changes in the Mechanical Properties of Alginate-Gelatin Hydrogels with the Addition of Pygeum africanum with Potential Application in Urology

Changes in the Mechanical Properties of Alginate-Gelatin Hydrogels with the Addition of Pygeum africanum with Potential Application in Urology

Investigation of co‐electrospun gelatin:TiO₂/polycaprolactone:silk fibroin scaffolds for wound healing applications

Glutaraldehyde: Introducing Optimum Condition for Cross-linking the Chitosan/Gelatin Scaffolds for Bone Tissue Engineering

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The physicochemical and mechanical investigation of siloxane modified Gelatin/Sodium alginate injectable hydrogels loaded by ascorbic acid and β-Glycerophosphate

Cited by 9 publications

References 61 publications

Changes in the Mechanical Properties of Alginate-Gelatin Hydrogels with the Addition of Pygeum africanum with Potential Application in Urology

Changes in the Mechanical Properties of Alginate-Gelatin Hydrogels with the Addition of Pygeum africanum with Potential Application in Urology

Investigation of co‐electrospun gelatin:TiO2/polycaprolactone:silk fibroin scaffolds for wound healing applications

Glutaraldehyde: Introducing Optimum Condition for Cross-linking the Chitosan/Gelatin Scaffolds for Bone Tissue Engineering

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Investigation of co‐electrospun gelatin:TiO₂/polycaprolactone:silk fibroin scaffolds for wound healing applications