The phylogenetic position of the endemic flat-needle pinePinus krempfii (Pinaceae) from Vietnam, based on PCR-RFLP analysis of chloroplast DNA

Wang, Xiaoru; Szmidt, Alfred E.; Nguyen, Hoang Nghia

doi:10.1007/bf00985368

Cited by 30 publications

(34 citation statements)

References 40 publications

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“…Thus, only the presence or absence of a restriction fragment was scored for all restriction patterns. Although restriction site analysis is often preferred in phylogenetic studies, comparative studies (Wang et al 2000;van Droogenbroeck et al 2004) show agreement with restriction fragment analysis. In fact, for example in Vasconcellea, fragment analysis showed even better resolution, likely due to the extra information derived from the length mutations (van Droogenbroeck et al 2004).…”

Section: Pcr Amplifications and Restriction Of Pcr Productsmentioning

confidence: 81%

“…Although the resolution and sampling in this current study is in some cases not sufficient to permit major conclusions of phylogeny and biogeography in Hypericum, the results partially corroborate previous classifications of Hypericum species, suggest some of novel relations and provide some clues for further studies in this genus. As pointed out by Wolfe & Liston (1998) and Wang et al (2000), PCR-RFLPs can be used as a selection for informative regions to be subjected to detailed sequence analysis. Comparisons between phylogenies inferred from both nuclear and chloroplast genomes would provide a better basis for assessing species relationships in Hypericum.…”

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confidence: 99%

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RFLP analysis of cpDNA in the genus Hypericum

et al. 2010

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The chloroplast DNA of 43 species including 16 sections from the genus Hypericum was studied by PCR-RFLP analysis. The PCR-amplified products of four cpDNA regions, trnC-trnD, psbC-trnS, trnL-trnF and rbcL were digested with four restriction endonucleases. A high level of interspecific variation was detected while intraspecific diversity was not observed. The resulting parsimony analysis indicated the monophyletic assemblage of the sections Androsaemum, Olympia, Drosocarpium and Trigynobrathys. Monophyly of Hypericum is weakly supported, but close relationships of H. perforatum and H. maculatum are indicated. The members of Ascyreia are weakly resolved, but clustering of H. kouytchense and H. oblongifolium is well supported, however, H. reptans is nested with Olympia. CpDNA profiles and the positions on the parsimony tree indicate that the chloroplast donor among the putative parents of the hybrid species H. ×inodorum is H. androsaemum.

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Section: Pcr Amplifications and Restriction Of Pcr Productsmentioning

confidence: 81%

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confidence: 99%

RFLP analysis of cpDNA in the genus Hypericum

et al. 2010

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“…The source phylogenies we used for supertree construction were based on molecular and morphological data. We used 11 of the 14 published phylogenies used by Schwilk and Ackerly (2001;namely Wheeler et al 1983;Millar et al 1988;Karalamangala and Nickrent 1989;Strauss and Doerksen 1990;Govindaraju et al 1992;Malusa 1992;Wang and Szmidt 1993;Krupkin et al 1996;Adams and Jackson 1997;Liston et al 1999 [P. krempfii was omitted due to possible misidentification]; Wang et al 1999) along with nine additional phylogenies (Schirone et al 1991;Shurkhal et al 1992;Perez de la Rosa et al 1995;Farjon 1996;Furman et al 1997;Wang et al 2000;Dvorak et al 2000;Gernandt et al 2001;Nkongolo et al 2002). We did not consider three phylogenies used by Schwilk and Ackerly (2001) because they represented only two or three species and/or had no outgroups.…”

Section: Constructing the Pinus Supertreementioning

confidence: 99%

Evolution of Genome Size in Pines (Pinus) and Its Life-History Correlates: Supertree Analyses

et al. 2004

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Abstract. Genome size has been suggested to be a fundamental biological attribute in determining life-history traits in many groups of organisms. We examined the relationships between pine genome sizes and pine phylogeny, environmental factors (latitude, elevation, annual rainfall), and biological traits (latitudinal and elevational ranges, seed mass, minimum generation time, interval between large seed crops, seed dispersal mode, relative growth rate, measures of potential and actual invasiveness, and level of rarity). Genome sizes were determined for 60 pine taxa and then combined with published values to make a dataset encompassing 85 species, or 70% of species in the genus. Supertrees were constructed using 20 published source phylogenies. Ancestral genome size was estimated as 32 pg. Genome size has apparently remained stable or increased over evolutionary time in subgenus Strobus, while it has decreased in most subsections in subgenus Pinus. We analyzed relationships between genome size and life-history variables using cross-species correlations and phylogenetically independent contrasts derived from supertree constructions. The generally assumed positive relation between genome size and minimum generation time could not be confirmed in phylogenetically controlled analyses. We found that the strongest correlation was between genome size and seed mass. Because the growth quantities specific leaf area and leaf area ratio (and to a lesser extent relative growth rate) are strongly negatively related to seed mass, they were also negatively correlated with genome size. Northern latitudinal limit was negatively correlated with genome size. Invasiveness, particularly of wind-dispersed species, was negatively associated with both genome size and seed mass. Seed mass and its relationships with seed number, dispersal mode, and growth rate contribute greatly to the differences in life-history strategies of pines. Many life-history patterns are therefore indirectly, but consistently, associated with genome size.

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“…This species can only grow in restricted habitats at higher altitudes in the border areas of Dak Lak, Lam Dong, and Khanh Hoa Provinces. Although its estimated extent of occurrence is within the threshold for endangered species, and its area of occupancy is also likely to be less than 500 km 2 , there are more than five locations known and the subpopulations are not currently considered severely fragmented (Wang et al, 2000;Thomas et al, 2013). P. krempfii grows as a large emergent tree at altitudes between 1200-2000 m; it is always at the top or on the upper slopes of flattened ridges.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, illegal logging aimed at P. krempfii in Chu Yang Sin has recently been reported (Thomas et al, 2013). Genetic markers have been used to study the phylogenetic position of P. krempfii and have revealed its status as a member of the subgenus Strobus (Willyard et al, 2007;Parks et al, 2012;Wang et al, 2000Wang et al, , 2014. However, only limited individuals of P. krempfii were included in these studies, and the levels and patterns of DNA variation within natural populations remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the endemic flat-needle pine Pinus krempfii (Pinaceae) from Vietnam revealed by SSR markers

Phòng¹,

Lieu²,

Hiền³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Pinus krempfii Lecomte (Pinaceae) is an endemic tree to Vietnam with restricted habitats at higher altitudes in the highlands. In this study, genetic variation of four populations of P. krempfii was assessed using 17 microsatellite markers (single sequence repeats). Of these 17 markers, eight were polymorphic, and among the 42 putative alleles amplified, 32 were polymorphic (accounting for 76.19%). The Cong Troi population was found to be the most genetically diverse (Shannon's information index, I = 0.415, and percentage of polymorphic bands, PPB = 52.95%), whereas the Hon Giao population was found to have the lowest diversity (I = 0.330 and PPB = 47.06%). The genetic diversity at species level was also estimated (I = 0.414, PPB = 76.19%). Molecular variance was found to be low among populations (11.94%) and high among individuals within the populations (88.06%). Private alleles were not detected in the Hon Giao population. The Yang Ly population

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The phylogenetic position of the endemic flat-needle pinePinus krempfii (Pinaceae) from Vietnam, based on PCR-RFLP analysis of chloroplast DNA

Cited by 30 publications

References 40 publications

RFLP analysis of cpDNA in the genus Hypericum

RFLP analysis of cpDNA in the genus Hypericum

Evolution of Genome Size in Pines (Pinus) and Its Life-History Correlates: Supertree Analyses

Genetic diversity of the endemic flat-needle pine Pinus krempfii (Pinaceae) from Vietnam revealed by SSR markers

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