RFLP analysis of cpDNA in the genus Hypericum

Pilepić, Kroata Hazler; Morović, Miranda; Orač, Filip; Šantor, Marija; Vejnović, Vanja

doi:10.2478/s11756-010-0101-z

Cited by 9 publications

(6 citation statements)

References 34 publications

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“…Although only six species representing five sections were included for phylogenetic analysis, relationship among those sections is comparable with previous studies (Crockett et al, 2004;Pilepić et al, 2010). For example, the close relationship between H. ascyron and H. hookerianum was reported by Pilepić et al (2010) and here we recovered the relationship. In addition, there are morphological correlates with the phylogenetic relationships.…”

Section: Resultssupporting

confidence: 87%

“…Numbers on each branch represent bootstrap support values (BS). (Pilepić et al, 2010;Meseguer et al, 2013;Nürk et al, 2013). In total, only five DNA markers (psbA-trnH, trnL-trnF, trnS-trnG, rbcL, and internal transcribed spacer) were used in these studies and not generated phylogenetic trees with high resolution, the part reason is due to lacking of variability within these DNA markers.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of the complete chloroplast genome sequence of Hypericum petiolulatum (Hypericaceae)

ZOU,

BA,

XIANG

et al. 2024

Korean J. Pl. Taxon

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Hypericum L. (Hypericaceae) is one of the best-selling herbal medicines in the world comprising ca. 500 species of herbs, shrubs, and small trees. Hypericum petiolulatum Hook. f. & Thomson ex Dyer is widely distributed in China, Vietnam, Myanmar, Nepal, India, Malaysia, and Bhutan and is used as a traditional herb to treat hemoptysis and inflammation. In this study, we sequenced and assembled the complete chloroplast (cp) genome of H. petiolulatum. The complete plastome of H. petiolulatum was 136,105 bp in length, with a large single copy region (LSC) of 93,709 bp, a small single copy region (SSC) of 11088 bp and two identical inverted repeats (IRs) of 15,654 bp. The overall GC content of the plastome was 37.0%, while GC contents of the LSC, SSC, and each IR were 35.5%, 31.0%, and 43.8%, respectively. In addition, 116 genes consisting of 76 protein-coding genes, six ribosomal RNA genes, and 34 transfer RNA genes were identified. A phylogenetic analysis of 14 taxa inferred based on cp genome sequences revealed a close relationship between H. petiolulatum and H. perforatum. The complete cp genome sequence of H. petiolulatum reported in this paper will facilitate population and phylogenomics studies of this medicinal plant group.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Characterization of the complete chloroplast genome sequence of Hypericum petiolulatum (Hypericaceae)

ZOU,

BA,

XIANG

et al. 2024

Korean J. Pl. Taxon

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show abstract

“…This theory is also supported by the fact that both H. attenuatum and H. maculatum have 16 chromosomes (2 n = 16) while H. perforatum contains 32 chromosomes (2 n = 32) . Alternatively, based on DNA differences with H. attenuatum , it is suggested that H. perforatum may have originated from H. maculatum alone The only way to shed light to the H. perforatum origin is DNA techniques.…”

Section: Dna Barcodingmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2017

Journal of Pharmacy and Pharmacology

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Objectives The most widely applied qualitative and quantitative analytical methods in the quality control of Hypericum perforatum extracts will be reviewed, including routine analytical tools and most modern approaches. Key findings Biologically active components of H. perforatum are chemically diverse; therefore, different chromatographic and detection methods are required for the comprehensive analysis of St. John's wort extracts. Naphthodianthrones, phloroglucinols and flavonoids are the most widely analysed metabolites of this plant. For routine quality control, detection of major compounds belonging to these groups seems to be sufficient; however, closer characterization requires the detection of minor compounds as well. Conclusions TLC and HPTLC are basic methods in the routine analysis, whereas HPLC‐DAD is the most widely applied method for quantitative analysis due to its versatility. LC‐MS is gaining importance in pharmacokinetic studies due to its sensitivity. Modern approaches, such as DNA barcoding, NIRS and NMR metabolomics, may offer new possibilities for the more detailed characterization of secondary metabolite profile of H. perforatum extracts.

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“…The total polyphenol content in our samples was about 50 µg GAE/mg dw and did not differ significantly from the leaves dried in different ways. The measured amount is higher than in other medicinal plants, such as Hypericum perforatum L. [41,42], Gynostemma pentaphyllum L. [43] or Micromeria croatica (Pers.) Schott [44], which were previously measured using similar protocols.…”

Section: Discussionmentioning

confidence: 60%

Influence of Air Drying, Freeze Drying and Oven Drying on the Biflavone Content in Yellow Ginkgo (Ginkgo biloba L.) Leaves

Jurčević Šangut,

Pavličević,

Šamec

2024

Applied Sciences

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Drying herbs is a crucial method for stabilizing and preserving their essential properties and bioactive compounds. Although freeze drying is the preferred method for most herbs, it is expensive due to high energy consumption and operating costs. Biflavonoids are dimeric flavonoids that have recently been recognized as potential molecules possessing biological activities, such as antiviral and antimicrobial activity, and as effective molecules for the treatment of neurodegenerative and metabolic diseases and for cancer therapies. In this study, we performed a comparative analysis of freeze drying, air drying and oven drying to evaluate their effects on biflavonoid content in yellow ginkgo leaves (Ginkgo biloba L.). After drying, we performed spectrophotometric analysis to determine the browning index, pigments, phenolic compounds and antioxidant activity, while HPLC-DAD was used for the identification and quantification of individual biflavones (amentoflavone, bilobetin, ginkgetin, isoginkgetin and sciadopitysin). The most abundant biflavonoids were isoginkgetin and bilobetin, the amounts of which exceeded 1000 µg/g dw in all leaf samples. They were followed by ginkgetin and sciadopitysin, the amounts of which were about 30% lower. The drying method did not influence biflavone content or the total carotenoids, total polyphenols and total flavonoids. Consequently, our study suggests that all three methods may be used for the preparation of yellow ginkgo leaves as a source of biflavones and other bioactive compounds.

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RFLP analysis of cpDNA in the genus Hypericum

Cited by 9 publications

References 34 publications

Characterization of the complete chloroplast genome sequence of <i>Hypericum petiolulatum</i> (Hypericaceae)

Characterization of the complete chloroplast genome sequence of <i>Hypericum petiolulatum</i> (Hypericaceae)

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Influence of Air Drying, Freeze Drying and Oven Drying on the Biflavone Content in Yellow Ginkgo (Ginkgo biloba L.) Leaves

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