The Phylogenetic Emergence of Vertebrate Immunity

Marchalonis, John J.; Cone, Robert E.

doi:10.1038/icb.1973.44

Cited by 51 publications

(40 citation statements)

References 20 publications

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“…After iodination the cells were incubated under short-term cell-culture conditions for 2-6 hr as described (13,18). Under these conditions cell-surface proteins, including immunoglobulin, are released into the cell-culture medium (13,18).…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, 3-month-old (CBA x C57BL)F1 or (CBA x BALB/c)F1 mice were injected intravenously with 2 X 108 CBA thymus cell's within 2 hr after receiving 750 R total body irradiation. 4 Days later the thoracic ducts of the mice were cannulated, and lymph was collected over the first [12][13][14][15][16] hr of drainage. 95-100% of thoracic-duct lymphocytes obtained in this way are cells of donor-thymus origin.…”

Section: Methodsmentioning

confidence: 99%

“…This objection would be obviated if it were demonstrated that surface immunoglobulin extracted from lymphocytes specifically reactive to a certain antigen could bind specifically to that antigen. Such an approach has become feasible because lactoperoxidase (EC 1.11.1.7)-catalyzed radioiodination (8) of cell surface proteins (9-11) has enabled the isolation and partial characterization of surface immunoglobulin molecules from thymus-influenced (T) lymphocytes (12, 13) and bursa or bone-marrow-derived (B) lymphocytes (11)(12)(13)(14) Sprent and Miller (17). Briefly, 3-month-old (CBA x C57BL)F1 or (CBA x BALB/c)F1 mice were injected intravenously with 2 X 108 CBA thymus cell's within 2 hr after receiving 750 R total body irradiation.…”

Section: Introductionmentioning

confidence: 99%

“…Aliquots were removed at intervals and centrifuged at 40 at 1500 rpm (500 X g) for 10 min, and the supernatants were retained. Radioactive cell-surface immunoglobulin was isolated from the supernatants by specific coprecipitation with antiserum against mouse immunoglobulin prepared in rabbits and purified mouse IgG as carrier (12,13 …”

mentioning

confidence: 99%

“…4 Days later the thoracic ducts of the mice were cannulated, and lymph was collected over the first [12][13][14][15][16] (9), except that the total reaction volume was 50 Al. We have previously shown that this technique iodinates cell-surface proteins exclusively but does not affect the viability of the cells (9).After iodination the cells were incubated under short-term cell-culture conditions for 2-6 hr as described (13,18). Under these conditions cell-surface proteins, including immunoglobulin, are released into the cell-culture medium (13, 18).…”

mentioning

confidence: 99%

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Antigen-Binding Specificity of Isolated Cell-Surface Immunoglobulin from Thymus Cells Activated to Histocompatibility Antigens

Cone

Sprent

Marchalonis

1972

Proc. Natl. Acad. Sci. U.S.A.

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Lactoperoxidase-catalyzed radioiodination of cell-surface proteins was used in the isolation of cellsurface immunoglobulin from thymus-derived thoracic duct lymphocytes activated to histocompatibility-2 antigens. Immunoglobulin was identified by specific precipitation with antiserum to mouse immunoglobulin prepared in the rabbit and mouse immunoglobulin. Polyacrylamide gel electrophoresis of reduced and alkylated precipitates showed that the immunoglobulin molecules possessed I-type heavy chains and light chains. Cell-surface immunoglobulin isolated from thymus-derived cells activated to histocompatibility-2 antigens possessed binding specificity for the activating antigens.The ability of antisera to immunoglobulin to inhibit the immunocompetence of lymphocytes has been considered to provide evidence that specific interaction of these cells with antigen is mediated by immunoglobulin-like receptors located on the cell surface (1-7). Such studies, however, do not exclude the possibility that receptor sites are not immunoglobulin in nature but are situated sufficiently close to immunoglobulin molecules for antibodies directed against immunoglobulins to block, by steric hindrance, the access of antigen to receptor sites. This objection would be obviated if it were demonstrated that surface immunoglobulin extracted from lymphocytes specifically reactive to a certain antigen could bind specifically to that antigen. Such an approach has become feasible because lactoperoxidase (EC 1.11.1.7)-catalyzed radioiodination (8) of cell surface proteins (9-11) has enabled the isolation and partial characterization of surface immunoglobulin molecules from thymus-influenced (T) lymphocytes (12, 13) and bursa or bone-marrow-derived (B) lymphocytes (11)(12)(13)(14) Sprent and Miller (17). Briefly, 3-month-old (CBA x C57BL)F1 or (CBA x BALB/c)F1 mice were injected intravenously with 2 X 108 CBA thymus cell's within 2 hr after receiving 750 R total body irradiation. 4 Days later the thoracic ducts of the mice were cannulated, and lymph was collected over the first [12][13][14][15][16] (9), except that the total reaction volume was 50 Al. We have previously shown that this technique iodinates cell-surface proteins exclusively but does not affect the viability of the cells (9).After iodination the cells were incubated under short-term cell-culture conditions for 2-6 hr as described (13,18). Under these conditions cell-surface proteins, including immunoglobulin, are released into the cell-culture medium (13, 18). Aliquots were removed at intervals and centrifuged at 40 at 1500 rpm (500 X g) for 10 min, and the supernatants were retained. Radioactive cell-surface immunoglobulin was isolated from the supernatants by specific coprecipitation with antiserum against mouse immunoglobulin prepared in rabbits and purified mouse IgG as carrier (12,13

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Antigen-Binding Specificity of Isolated Cell-Surface Immunoglobulin from Thymus Cells Activated to Histocompatibility Antigens

Cone

Sprent

Marchalonis

1972

Proc. Natl. Acad. Sci. U.S.A.

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Immunoglobulins on the surface of sheep lymphocytes. ii. class, size and fate during incubation of lymphocytes at 37 °c

1973

Eur J Immunol

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Purification of an antibody‐like protein from the sea star Asterias rubens (L.)

et al. 1986

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Cells from sea star (Asterias rubens) axial organs stimulated with trinitrophenyl (TNP) or fluoresceinyl-haptened polyacrylamide beads and subsequently stimulated in vitro with the same antigen produced and released a specific antibody-like protein which induced lysis of haptened sheep erythrocytes in the presence of serum complement. The anti-TNP antibody-like protein isolated by ammonium sulfate precipitation, gel filtration and affinity chromatography exhibited a single precipiting peak after crossed immunoelectrophoresis against rabbit antiserum to partially purified culture supernatant. The anti-TNP antibody-like protein gave a specific affinity precipitate in crossed affino-electrophoresis using a p-nitrobenzoyl-substituted gel. The analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions evidenced a unique 30-kDa polypeptide chain. According to gel filtration experiments, the molecular weight of the major component isolated by affinity chromatography was about four times higher. Therefore, the antibody-like molecule could be a tetrameric protein devoid of any disulfide bond.

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The Phylogenetic Emergence of Vertebrate Immunity

Cited by 51 publications

References 20 publications

Antigen-Binding Specificity of Isolated Cell-Surface Immunoglobulin from Thymus Cells Activated to Histocompatibility Antigens

Antigen-Binding Specificity of Isolated Cell-Surface Immunoglobulin from Thymus Cells Activated to Histocompatibility Antigens

Immunoglobulins on the surface of sheep lymphocytes. ii. class, size and fate during incubation of lymphocytes at 37 °c

Purification of an antibody‐like protein from the sea star Asterias rubens (L.)

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