Immunoglobulins on the surface of sheep lymphocytes. ii. class, size and fate during incubation of lymphocytes at 37 °c

Ey, Peter L.

doi:10.1002/eji.1830030706

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1974

1987

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Cited by 8 publications

(1 citation statement)

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“…These results are in agreement with recent findings obtained by other workers using radioiodine as a label [16][17][18][19]. Furthl more, in the present work we showed that antibody and Fab conjugates led to a similar fate of cell surface Ig; but with Fab fragment pinocytosis was less pronounced and elution was more marked than with whole antibody.…”

Section: Discussionsupporting

confidence: 94%

Surface immunoglobulins of rat immunocytes: Quantitation and fate of cell‐bound peroxidase‐labeled antibody and Fab fragment

Antoine

Avraméas

1974

Eur J Immunol

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Section: Discussionsupporting

confidence: 94%

Surface immunoglobulins of rat immunocytes: Quantitation and fate of cell‐bound peroxidase‐labeled antibody and Fab fragment

Antoine

Avraméas

1974

Eur J Immunol

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Rapid recovery of antigen‐binding receptors on chicken B cells following anti‐lg serum treatment

Iványi¹,

Fuensalida²,

Lydyard³

1976

Eur J Immunol

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Rosette-forming cells (RFC) from the peripheral blood of sheep red blood cell (SRBC)-immunized chickens were characterized as B cells which manifest antigen-binding receptors but no antibody secretion. Lymphocytes were pretreated at 0 degrees C with anti-Ig serum, washed, incubated further at various temperatures and harvested for the rosette-forming cells (RFC) assay. Anti-Ig treatment blocked all RFC formation and providing that the cells were maintained at less than 10 degrees C regeneration did not occur up to 6 h. Complete recovery of RFC formation occurred within 10 min at 37 degrees C following treatment with low but not with high concentrations of anti-Ig serum. However, recovery from inhibition by high-dose anti-Ig treatment was achieved by including chicken IgG in the incubation medium. The recovery of receptors was reduced following "sandwich" treatment of lymphocytes. When cells were treated with anti-Ig antibody, washed and exposed to various drug inhibitors of "capping" the regeneration of RFC was prevented; in contrast, inhibitors of protein synthesis were ineffective. The results were interpreted in terms of dissociation of anti-Ig antibodies from the Ig receptor at an early stage of the ligand-induced receptor redistribution process.

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Calf thymus alkaline phosphatase. II. Interaction with detergents

Ey¹,

Ferber²

1977

Biochimica et Biophysica Acta (BBA) - Enzymology

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Immunoglobulins on the surface of sheep lymphocytes. ii. class, size and fate during incubation of lymphocytes at 37 °c

Cited by 8 publications

References 33 publications

Surface immunoglobulins of rat immunocytes: Quantitation and fate of cell‐bound peroxidase‐labeled antibody and Fab fragment

Surface immunoglobulins of rat immunocytes: Quantitation and fate of cell‐bound peroxidase‐labeled antibody and Fab fragment

Rapid recovery of antigen‐binding receptors on chicken B cells following anti‐lg serum treatment

Calf thymus alkaline phosphatase. II. Interaction with detergents

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