DOI: 10.31274/rtd-180813-6075
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The phosphorylation of bovine heart glycogen synthase

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“…During myocardial reperfusion injury, opening of the mitochondrial permeability transition pore (MPTP) collapses ΔΨ m and uncouples oxidative phosphorylation, resulting in ATP depletion and apoptosis (Beltran, Mathur et al 2000; Hausenloy and Yellon 2003). Fluid shear stress is reported to influence mitochondrial ATP synthesis, which is coupled with ΔΨ m (Mitchell and Thomas 1979; Kudo, Morigaki et al 2000). The formation of mitochondrial ROS (mtROS) is dependent on ΔΨ m (Korshunov, Skulachev et al 1997), and mtROS level increases exponentially as ΔΨ m is increased or hyperpolarized above -140 mV (Lee, Bender et al 2002).…”
Section: Basic Protocolmentioning
confidence: 99%
“…During myocardial reperfusion injury, opening of the mitochondrial permeability transition pore (MPTP) collapses ΔΨ m and uncouples oxidative phosphorylation, resulting in ATP depletion and apoptosis (Beltran, Mathur et al 2000; Hausenloy and Yellon 2003). Fluid shear stress is reported to influence mitochondrial ATP synthesis, which is coupled with ΔΨ m (Mitchell and Thomas 1979; Kudo, Morigaki et al 2000). The formation of mitochondrial ROS (mtROS) is dependent on ΔΨ m (Korshunov, Skulachev et al 1997), and mtROS level increases exponentially as ΔΨ m is increased or hyperpolarized above -140 mV (Lee, Bender et al 2002).…”
Section: Basic Protocolmentioning
confidence: 99%