The phenylpropanoid pathway inhibitor piperonylic acid induces broad‐spectrum pest and disease resistance in plants

Desmedt, Willem; Jonckheere, Wim; Nguyễn, Việt Hà; Ameye, Maarten; Zutter, Noémie De; Kock, Karen De; Debode, Jane; Leeuwen, Thomas Van; Audenaert, Kris; Vanholme, Bartel; Kyndt, Tina

doi:10.1111/pce.14119

Cited by 43 publications

(35 citation statements)

References 96 publications

(145 reference statements)

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“…Lignified walls prevent pathogen infections by acting as a non-degradable mechanical barrier. The regulation of secondary metabolism genes in BRS may be a response to nematode migration and necrosis, as seen in plant–herbivore interactions [ 66 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

Time Course RNA-seq Reveals Soybean Responses against Root-Lesion Nematode and Resistance Players

Lopes-Caitar

Nomura

Hishinuma-Silva

et al. 2022

Plants

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Pratylenchus brachyurus causes serious damage to soybean production and other crops worldwide. Plant molecular responses to RLN infection remain largely unknown and no resistance genes have been identified in soybean. In this study, we analyzed molecular responses to RLN infection in moderately resistant BRSGO (Chapadões—BRS) and susceptible TMG115 RR (TMG) Glycine max genotypes. Differential expression analysis revealed two stages of response to RLN infection and a set of differentially expressed genes (DEGs) in the first stage suggested a pattern-triggered immunity (PTI) in both genotypes. The divergent time-point of DEGs between genotypes was observed four days post-infection, which included the activation of mitogen-activated protein kinase (MAPK) and plant–pathogen interaction genes in the BRS, suggesting the occurrence of an effector-triggered immunity response (ETI) in BRS. The co-expression analyses combined with single nucleotide polymorphism (SNP) uncovered a key element, a transcription factor phytochrome-interacting factor (PIF7) that is a potential regulator of moderate resistance to RLN infection. Two genes for resistance-related leucine-rich repeat (LRR) proteins were found as BRS-specific expressed genes. In addition, alternative splicing analysis revealed an intron retention in a myo-inositol oxygenase (MIOX) transcript, a gene related to susceptibility, may cause a loss of function in BRS.

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Section: Discussionmentioning

confidence: 99%

Time Course RNA-seq Reveals Soybean Responses against Root-Lesion Nematode and Resistance Players

Lopes-Caitar

Nomura

Hishinuma-Silva

et al. 2022

Plants

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“…The plant-pathogen interaction and defense response would help DXWR survive under biotic stress ( Kushalappa and Gunnaiah, 2013 ). Phenylpropanoid which was a rich source of secondary metabolites in plants, together with plant hormone, played important roles in plant growth, development, and defense ( Desmedt et al, 2021 ; Dong and Lin, 2021 ). Moreover, starch and sucrose metabolism was the vital pathway in the process of rice grain filling, which could finally affect the yield ( Fan et al, 2019 ; Jiang et al, 2021 ; Mathan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Chromosome-level genome assembly of Dongxiang wild rice (Oryza rufipogon) provides insights into resistance to disease and freezing

Zhi-qun

Shen

2022

Front. Genet.

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Dongxiang wild rice (DXWR, Oryza rufipogon Griff.) belongs to common wild rice O. rufipogon, which is the well-known ancestral progenitor of cultivated rice, possessing important gene resources for rice breeding. However, the distribution of DXWR is decreasing rapidly, and no reference genome has been published to date. In this study, we constructed a chromosome-level reference genome of DXWR by Oxford Nanopore Technology (ONT) and High-through chromosome conformation capture (Hi-C). A total of 58.41 Gb clean data from ONT were de novo assembled into 231 contigs with the total length of 413.46 Mb and N50 length of 5.18 Mb. These contigs were clustered and ordered into 12 pseudo-chromosomes covering about 97.39% assembly with Hi-C data, with a scaffold N50 length of 33.47 Mb. Moreover, 54.10% of the genome sequences were identified as repeat sequences. 33,862 (94.21%) genes were functionally annotated from a total of predicted 35,942 protein-coding sequences. Compared with other species of Oryza genus, the genes related to disease and cold resistance in DXWR had undergone a large-scale expansion, which may be one of the reasons for the stronger disease resistance and cold resistance of DXWR. Comparative transcriptome analysis also determined a list of differentially expressed genes under normal and cold treatment, which supported DXWR as a cold-tolerant variety. The collinearity between DXWR and cultivated rice was high, but there were still some significant structural variations, including a specific inversion on chromosome 11, which may be related to the differentiation of DXWR. The high-quality chromosome-level reference genome of DXWR assembled in this study will become a valuable resource for rice molecular breeding and genetic research in the future.

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“…Based on the observation that blocking PAL does not enhance susceptibility, it is likely that different metabolites could even play opposite roles in plant susceptibility or defense. Our recent research revealed that temporary perturbation of the phenylpropanoid pathway leads to a general reprogramming of the plant defense metabolome, and broad-spectrum-induced resistance in tomato (Desmedt et al, 2021). Which metabolite(s) is/are responsible for this IR remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes

et al. 2021

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Anticipating an increased ecological awareness, scientists have been exploring new strategies to reduce the use of chemical pesticides to control pests and diseases. Triggering the intrinsic plant defense system is one of the promising strategies to reduce yield loss by pathogenic organisms, such as nematodes. Ascorbate oxidase (AO) enzyme plays an important role in plant defense by regulating the apoplastic ascorbate/dehydroascorbate (DHA) ratio via the ascorbate oxidation process. Ascorbate oxidation is known to induce systemic resistance in rice against parasitic root-knot nematodes (RKN). Here, we sought to evaluate if AO- or DHA-induced resistance (IR) against RKN M. graminicola involves activation of the phenylpropanoid pathway and whether this IR phenotype has potential effects on growth of rice seedlings under stressed and unstressed conditions. Our results show that AO/DHA-IR against these parasitic nematodes is dependent on activation of phenylalanine ammonia lyase (PAL). However, application of reduced ascorbic acid (AA) did not induce this response. Gene expression analysis via qRT-PCR showed that OsPAL2 and OsPAL4 are highly expressed in AO/DHA-sprayed nematode-infected roots and PAL-activity measurements confirmed that AO/DHA spraying triggers the plants for primed activation of this enzyme upon nematode infection. AO/DHA-IR is not effective in plants sprayed with a chemical PAL inhibitor confirming that AO/DHA-induced resistance is dependent on PAL activity. Improved plant growth and low nematode infection in AO/DHA-sprayed plants was found to be correlated with an increase in shoot chlorophyll fluorescence (Fv/Fm), chlorophyll index (ChlIdx), and modified anthocyanin reflection index which were proven to be good above-ground parameters for nematode infestation. A detailed growth analysis confirmed the improved growth of AO/DHA-treated plants under nematode-infected conditions. Taken together, our results indicate that ascorbate oxidation enhances the phenylpropanoid-based response to nematode infection and leads to a tolerance phenotype in treated rice plants.

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The phenylpropanoid pathway inhibitor piperonylic acid induces broad‐spectrum pest and disease resistance in plants

Cited by 43 publications

References 96 publications

Time Course RNA-seq Reveals Soybean Responses against Root-Lesion Nematode and Resistance Players

Time Course RNA-seq Reveals Soybean Responses against Root-Lesion Nematode and Resistance Players

Chromosome-level genome assembly of Dongxiang wild rice (Oryza rufipogon) provides insights into resistance to disease and freezing

Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes

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