Time Course RNA-seq Reveals Soybean Responses against Root-Lesion Nematode and Resistance Players

Abstract

Pratylenchus brachyurus causes serious damage to soybean production and other crops worldwide. Plant molecular responses to RLN infection remain largely unknown and no resistance genes have been identified in soybean. In this study, we analyzed molecular responses to RLN infection in moderately resistant BRSGO (Chapadões—BRS) and susceptible TMG115 RR (TMG) Glycine max genotypes. Differential expression analysis revealed two stages of response to RLN infection and a set of differentially expressed genes (DEGs) in the first stage suggested a pattern-triggered immunity (PTI) in both genotypes. The divergent time-point of DEGs between genotypes was observed four days post-infection, which included the activation of mitogen-activated protein kinase (MAPK) and plant–pathogen interaction genes in the BRS, suggesting the occurrence of an effector-triggered immunity response (ETI) in BRS. The co-expression analyses combined with single nucleotide polymorphism (SNP) uncovered a key element, a transcription factor phytochrome-interacting factor (PIF7) that is a potential regulator of moderate resistance to RLN infection. Two genes for resistance-related leucine-rich repeat (LRR) proteins were found as BRS-specific expressed genes. In addition, alternative splicing analysis revealed an intron retention in a myo-inositol oxygenase (MIOX) transcript, a gene related to susceptibility, may cause a loss of function in BRS.

Developing an SNP dataset for efficiently evaluating soybean germplasm resources using the genome sequencing data of 3,661 soybean accessions

“…These may be related to agronomic traits resulting from the domestication process (Tables S6, S7). For example, Glyma.17G128200 and Glyma.10G295400 are associated with soybean seed yield traits [ 27 , 28 ], Glyma.15G130000 is associated with soybean seed protein concentration [ 29 ], Glyma.11G092100 was demonstrated to directly influence the anabolism of sucrose in soybean [ 30 ], and Glyma.12G143200 and Glyma.13G285300 are associated with soybean cyst nematode and root-lesion nematode resistance, respectively [ 31 , 32 ].…”

Genome-Wide Association Analysis for Resistance to Coniothyrium glycines Causing Red Leaf Blotch Disease in Soybean

“…The leucine-rich repeat (LRR) family protein is well-known for controlling disease resistance in crops, including soybean [ 78 ]. In soybean, LRR has been reported to regulate the immune response to Phytophthora root rot [ 79 ], coordinate the responses against root-lesion nematode [ 80 ], and mediate the response to soybean mosaic virus [ 81 ]. This study also revealed the contribution of the LRR domain in the resistance to red leaf blotch disease in soybean production.…”