The Phenotypic Characteristic of Liver-Derived Stem Cells From Adult Human Deceased Donor Liver

Lee, J.-H.; Park, H.-J.; Kim, Y.-A.; Lee, D.-H.; Noh, Jeong-Kwon; Kwon, Choon Hyuck David; Jung, Sunghoon; Lee, S.-K.

doi:10.1016/j.transproceed.2012.02.020

Cited by 17 publications

(19 citation statements)

References 5 publications

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“…In terms of morphology, cultured LHMSCs exhibit an elongated spindle shape with ovoid nuclei (Fig. 1 ), as well as stem cell properties, including positivity for stem cell markers (vimentin and nestin) and MSC markers (CD29, CD73, CD44, CD90, CD105, and CD166) [ 21 ]. However, LHMSCs are negative for hematopoietic stem cell markers (CD34, CD45, CD117), suggesting that these cells are not of hematopoietic origin [ 19 , 22 ].…”

Section: Characterization Of Lhmscsmentioning

confidence: 99%

“…This observation suggests that LHMSCs may be at a more advanced stage of differentiation. Interestingly, LHMSCs express CD26, albumin, CK8, and CK18, indicating a partial commitment toward hepatic cell differentiation [ 21 , 22 , 24 ]. Similar to other MSCs, LHMSCs have low immunogenicity due to the absence of major histocompatibility complex (MHC) class II (human leukocyte antigen (HLA)-DP, -DQ, and -DR) antigens, FAS ligand or costimulatory molecules with the exception of MHC class I antigens [ 25 , 26 ].…”

Section: Characterization Of Lhmscsmentioning

confidence: 99%

“…LHMSCs are spindle-shaped, whereas liver progenitor cells are oval. Moreover, LHMSCs are negative for CD117, CD34, and CK19, markers of liver progenitor cells [ 21 ]. Hepatic stellate cells (HSCs) are another type of stem/progenitor cell in the liver that acquire a myofibroblast-like phenotype when activated.…”

Section: Characterization Of Lhmscsmentioning

confidence: 99%

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Liver-derived human mesenchymal stem cells: a novel therapeutic source for liver diseases

Wang

Chen

et al. 2016

Stem Cell Res Ther

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Mesenchymal stem cells (MSCs) represent an attractive cell type for research and therapy due to their ability to proliferate, differentiate, modulate immune reactions, and secrete trophic factors. MSCs exist in a multitude of tissues, including bone marrow, umbilical cord, and adipose tissues. Moreover, MSCs have recently been isolated from the liver. Compared with other MSC types, liver-derived human MSCs (LHMSCs) possess general morphologies, immune functions, and differentiation capacities. Interestingly, LHMCSs produce higher levels of pro-angiogenic, anti-inflammatory, and anti-apoptotic cytokines than those of bone marrow-derived MSCs. Thus, these cells may be a promising therapeutic source for liver diseases. This paper summarizes the biological characteristics of LHMSCs and their potential benefits and risks for the treatment of liver diseases.

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Section: Characterization Of Lhmscsmentioning

confidence: 99%

Section: Characterization Of Lhmscsmentioning

confidence: 99%

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Liver-derived human mesenchymal stem cells: a novel therapeutic source for liver diseases

Wang

Chen

et al. 2016

Stem Cell Res Ther

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“…Several studies have suggested the presence of stem cells in the adult normal human liver. A population positive to mesenchymal stem cell markers (CD29, CD73, CD44, and CD90), while expressing albumin and α-fetoprotein, was described in the early 2000s, indicating the possibility that a stem cell population may be present in the adult human liver [90][91][92]. However, this issue remains controversial, as AdHLSCs possess several advantages over adult hepatocytes or fetal progenitors as they are able to proliferate in vitro [93,94].…”

Section: Adult Human Liver Stem Cells (Adhlscs)mentioning

confidence: 99%

Pluripotent-Stem-Cell-Derived Hepatic Cells: Hepatocytes and Organoids for Liver Therapy and Regeneration

Messina

Luce

Hussein

et al. 2020

Cells

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The liver is a very complex organ that ensures numerous functions; it is thus susceptible to multiple types of damage and dysfunction. Since 1983, orthotopic liver transplantation (OLT) has been considered the only medical solution available to patients when most of their liver function is lost. Unfortunately, the number of patients waiting for OLT is worryingly increasing, and extracorporeal liver support devices are not yet able to counteract the problem. In this review, the current and expected methodologies in liver regeneration are briefly analyzed. In particular, human pluripotent stem cells (hPSCs) as a source of hepatic cells for liver therapy and regeneration are discussed. Principles of hPSC differentiation into hepatocytes are explored, along with the current limitations that have led to the development of 3D culture systems and organoid production. Expected applications of these organoids are discussed with particular attention paid to bio artificial liver (BAL) devices and liver bio-fabrication.

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“…The existence and basal characteristics of hLD-SCs were reported by Herrera et al in 2006 [ 29 ] and Najimi et al in 2007 [ 30 ]. Pan [ 31 ] and Lee [ 32 ] also reported similar hLD-SCs and investigated their characteristics. Cells with similar properties were observed in the rodent liver [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Hepatogenic Potential and Liver Regeneration Effect of Human Liver-derived Mesenchymal-Like Stem Cells

Lee

Choi

Kang

et al. 2020

Cells

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Human liver-derived stem cells (hLD-SCs) have been proposed as a possible resource for stem cell therapy in patients with irreversible liver diseases. However, it is not known whether liver resident hLD-SCs can differentiate toward a hepatic fate better than mesenchymal stem cells (MSCs) obtained from other origins. In this study, we compared the differentiation ability and regeneration potency of hLD-SCs with those of human umbilical cord matrix-derived stem cells (hUC-MSCs) by inducing hepatic differentiation. Undifferentiated hLD-SCs expressed relatively high levels of endoderm-related markers (GATA4 and FOXA1). During directed hepatic differentiation supported by two small molecules (Fasudil and 5-azacytidine), hLD-SCs presented more advanced mitochondrial respiration compared to hUC-MSCs. Moreover, hLD-SCs featured higher numbers of hepatic progenitor cell markers on day 14 of differentiation (CPM and CD133) and matured into hepatocyte-like cells by day 7 through 21 with increased hepatocyte markers (ALB, HNF4A, and AFP). During in vivo cell transplantation, hLD-SCs migrated into the liver of ischemia-reperfusion injury-induced mice within 2 h and relieved liver injury. In the thioacetamide (TAA)-induced liver injury mouse model, transplanted hLD-SCs trafficked into the liver and spontaneously matured into hepatocyte-like cells within 14 days. These results collectively suggest that hLD-SCs hold greater hepatogenic potential, and hepatic differentiation-induced hLD-SCs may be a promising source of stem cells for liver regeneration.

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The Phenotypic Characteristic of Liver-Derived Stem Cells From Adult Human Deceased Donor Liver

Cited by 17 publications

References 5 publications

Liver-derived human mesenchymal stem cells: a novel therapeutic source for liver diseases

Liver-derived human mesenchymal stem cells: a novel therapeutic source for liver diseases

Pluripotent-Stem-Cell-Derived Hepatic Cells: Hepatocytes and Organoids for Liver Therapy and Regeneration

Hepatogenic Potential and Liver Regeneration Effect of Human Liver-derived Mesenchymal-Like Stem Cells

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