The Phenotype of the Soybean Disease-Lesion Mimic (dlm) Mutant is Light-Dependent and Associated with Chloroplast Function

Kim, Byo-Kyong; Kim, Young‐Jin; Paek, Kyoung-Bee; Chung, Jong Il; Kim, Jeong-Kook

doi:10.5423/ppj.2005.21.4.395

Cited by 6 publications

(7 citation statements)

References 31 publications

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“…The dlm allele controlling disease lesion mimic trait is useful in basic research aimed at better understanding disease hypersensitive response and programmed cell death in soybean. Kim et al (2005) reported that dlm mutant was light-dependent and observed that the mutant showed a defect in starch degradation of chloroplasts during diurnal fluctuations. The position of dlm allele on linkage group were not reported.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of dlm and ti genes in Soybean

Sung¹,

Kim²,

Seo³

et al. 2013

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT dlm mutant newly identified displays necrotic spots with chlorotic halo despite the absence of any detectable pathogens in soybean. Kunitz trypsin inhibitor (KTI) protein of mature soybean seed is a main anti-nutritional factor and is controlled by Ti allele in soybean seed. The objective of this research was to determine the linkage or independent assortment between dlm allele and ti allele. F2 population was made by crossing between "Gaechuck2ho" (DlmDlmtiti) and T363 (dlmdlmTiTi) parents. The normal leaf or disease lesion mimic leaf trait of parents and F2 individual plants was recorded at R2 and R3 growth stage from field. The random seeds from individual F2 plants harvested were analysed electrophoretically to determine the presence of the KTI protein. The disease lesion mimic trait was matched to 3 : 1 ratio at the 0.01 levels of probability. The absence of the KTI protein was controlled by a single recessive gene. In F2 generation, the ratios of 102 Ti_Dlm_: 21 Ti_dlmdlm : 24 titiDlm_ : 4 titidlmdlm were observed. Segregation ratio gave a good fit to the 9 : 3 : 3 : 1 ratio based on F2 generation at the 0.01 levels of probability. The data confirmed that dlm allele controlling disease lesion mimic trait was inherited independently with the ti allele controlling absence of KTI protein in soybean.

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Section: Introductionmentioning

confidence: 99%

Inheritance of dlm and ti genes in Soybean

Sung¹,

Kim²,

Seo³

et al. 2013

Plant Breed. Biotech.

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“…The cell death phenotype of many lesion mimic mutants of Arabidopsis, maize, rice and soybean is dependent on light. [8][9][10][11][12][13] Similar to cultured mammalian cells, exposure to blue and ultra violet light stimulates production of reactive oxygen species in aleurone protoplasts resulting in cell death. 14 Similarly, light triggered production of excess porphyrin free radicals is responsible for cell death in maize plants mutated in the gene encoding uroporphyrinogen decarboxylase.…”

Section: Blue Light Photoreceptors Are Required For the Stability Andmentioning

confidence: 99%

Blue light photoreceptors are required for the stability and function of a resistance protein mediating viral defense in Arabidopsis

Jeong

Kachroo

2010

Plant Signaling & Behavior

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This light-perciving ability of plants requires the activities of proteins termed photoreceptors. In addition to various growth and developmental processes, light also plays a role in plant defense against pathogens and is required for activation of several defense genes and regulation of the cell death response. However, the molecular or biochemical basis of light modulated regulation of defense signaling is largely unclear. We demonstrate a direct role for blue-light photoreceptors in resistance (R) protein-mediated plant defense against Turnip Crinkle Virus (TCV) in Arabidopsis. The blue-light photoreceptors, cryptochrome (CRY) 2 and phototropin (PHOT) 2, are specifically required for maintaining the stability of the R protein HRT, and thereby resistance to TCV. Exogenous application of the phytohormone salicylic acid elevates HRT levels in phot2 but not in cry2 background. These data indicate that CRY2 and PHOT2 function distinctly in maintaining post-transcriptional stability of HRT. HRT-mediated resistance is also dependent on CRY1 and PHOT1 proteins, but these do not contribute to the stability of HRT. HRT interacts with the CRY2/PHOT2-interacting protein COP1, a E3 ubiquitin ligase. Exogenous application of a proteasome inhibitor prevents blue-light-dependent degradation of HRT, suggesting that HRT is degraded via the 26S proteasome. These and the fact that PHOT2 interacts directly with the R protein RPS2 suggest that blue-light photoreceptors might be involved in regulation and/or signaling mediated by several R proteins.

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“…; Kim et al . ). In these mutants, some mutations caused a HR‐like phenotype by manipulating ROS‐dependent irreversible cell damage, while some targeted the phytohormone signal for suppression of immunity responses, such as salicylic acid (SA) or jasmonic acid (JA) (Shah et al .…”

Section: Introductionmentioning

confidence: 97%

Semi‐dominant mutation in the cysteine‐rich receptor‐like kinase gene, ALS1, conducts constitutive defence response in rice

Liu

Xing

et al. 2018

Plant Biol J

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Plants have evolved a sophisticated two-branch defence system to prevent the growth and spread of pathogen infection. The novel Cys-rich repeat (CRR) containing receptor-like kinases, known as CRKs, were reported to mediate defence resistance in plants. For rice, there are only two reports of CRKs. A semi-dominant lesion mimic mutant als1 (apoptosis leaf and sheath 1) in rice was identified to demonstrate spontaneous lesions on the leaf blade and sheath. A map-based cloning strategy was used for fine mapping and cloning of ALS1, which was confirmed to be a typical CRK in rice. Functional studies of ALS1 were conducted, including phylogenetic analysis, expression analysis, subcellular location and blast resistance identification. Most pathogenesis-related (PR) genes and other defence-related genes were activated and up-regulated to a high degree. ALS1 was expressed mainly in the leaf blade and sheath, in which further study revealed that ALS1 was present in the vascular bundles. ALS1 was located in the cell membrane of rice protoplasts, and its mutation did not change its subcellular location. Jasmonic acid (JA) and salicylic acid (SA) accumulation were observed in als1, and enhanced blast resistance was also observed. The mutation of ALS1 caused a constitutively activated defence response in als1. The results of our study imply that ALS1 participates in a defence response resembling the common SA-, JA- and NH1-mediated defence responses in rice.

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The Phenotype of the Soybean Disease-Lesion Mimic (dlm) Mutant is Light-Dependent and Associated with Chloroplast Function

Cited by 6 publications

References 31 publications

Inheritance of dlm and ti genes in Soybean

Inheritance of dlm and ti genes in Soybean

Blue light photoreceptors are required for the stability and function of a resistance protein mediating viral defense in Arabidopsis

Semi‐dominant mutation in the cysteine‐rich receptor‐like kinase gene, ALS1, conducts constitutive defence response in rice

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