Inheritance of <i>dlm</i> and <i>ti</i> genes in Soybean

Sung, Mi Kyung; Kim, Min Hwan; Seo, Hyung Jin; Chung, Jong Il

doi:10.9787/pbb.2013.1.1.009

Cited by 3 publications

(3 citation statements)

References 15 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The segregation ratio for the presence or absence of lectin protein in the F2 seed generation was well fitted to an expected 3:1 ratio (χ 2 =2.28, P=0.5-0.01). These results substantiate earlier reports that Le locus is inherited as a single gene (Orf and Hymowitz 1979b;Sung et al 2013).…”

Section: Discussionsupporting

confidence: 82%

Breeding of Black Soybean Line with ti and le Allele

Choi¹,

Han²,

Sung³

et al. 2016

Plant Breed. Biotech.

Self Cite

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. P=0.5-0.1). From eight F2 seeds with double recessive allele (ti/ti, le/le genotype), one new strain (titilele, both KTI, and lectin protein free) was developed. New strain has black seed coat, black hilum and green cotyledon in mature seed and 100-seed weight was 19.5 g. This strain will be helpful in breeding program for selecting black soybean line with lacking both KTI and lectin protein.

show abstract

Section: Discussionsupporting

confidence: 82%

Breeding of Black Soybean Line with ti and le Allele

Choi¹,

Han²,

Sung³

et al. 2016

Plant Breed. Biotech.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, a 3:1 segregation ratio was observed (χ 2 = 2.59, P=0.5 -0.1) for the presence or absence of lectin protein in the F 2 seed generation. This result substantiate previous observations that lectin protein is controlled by a single gene (Choi et al 2016;Sung et al 2013).…”

Section: Discussionsupporting

confidence: 93%

Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon

et al. 2020

Self Cite

View full text Add to dashboard Cite

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F 2 seeds were obtained. A 3:1 segregation ratio was observed during F2 seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F 2 seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ 2 = 2.87, P=0.5-0.1). From nine F 2 seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.

show abstract

“…Subsequently, the dlm phenotype was found to be light-dependent and associated with chloroplast function [15]. Inheritance of dlm mutant phenotype and some leaf morphological traits were carried out, and it was reported the dlm allele inherited independently with that of P1 , y9 , f , lf2 and ti alleles controlling glabrous, chlorophyll-deficient leaf, stem trait, seven leaflet and trypsin inhibitors traits in soybean, respectively [17,18]. Wang et al [19] found a new LMM in soybean with rugose leaf phenotype controlled by two duplicated genes, rf1 , and rf2 , which were mapped on chromosome 18 and 08, respectively.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)

Golpour

Kong

Sharmin

et al. 2019

IJMS

View full text Add to dashboard Cite

In plants, lesion mimic mutants (LMMs) reveal spontaneous disease-like lesions in the absence of pathogen that constitutes powerful genetic material to unravel genes underlying programmed cell death (PCD), particularly the hypersensitive response (HR). However, only a few LMMs are reported in soybean, and no related gene has been cloned until now. In the present study, we isolated a new LMM named spotted leaf-1 (spl-1) from NN1138-2 cultivar through ethyl methanesulfonate (EMS) treatment. The present study revealed that lesion formation might result from PCD and excessive reactive oxygen species (ROS) accumulation. The chlorophyll content was significantly reduced but antioxidant activities, viz., superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), as well as the malondialdehyde (MDA) contents, were detected higher in spl-1 than in the wild-type. According to segregation analysis of mutant phenotype in two genetic populations, viz., W82×spl-1 and PI378692×spl-1, the spotted leaf phenotype of spl-1 is controlled by a single recessive gene named lm1. The lm1 locus governing mutant phenotype of spl-1 was first identified in 3.15 Mb genomic region on chromosome 04 through MutMap analysis, which was further verified and fine mapped by simple sequence repeat (SSR) marker-based genetic mapping. Genetic linkage analysis narrowed the genomic region (lm1 locus) for mutant phenotype to a physical distance of ~76.23 kb. By searching against the Phytozome database, eight annotated candidate genes were found within the lm1 region. qRT-PCR expression analysis revealed that, among these eight genes, only Glyma.04g242300 showed highly significant expression levels in wild-type relative to the spl-1 mutant. However, sequencing data of the CDS region showed no nucleotide difference between spl-1 and its wild type within the coding regions of these genes but might be in the non-coding regions such as 5′ or 3′ UTR. Hence, the data of the present study are in favor of Glyma.04g242300 being the possible candidate genes regulating the mutant phenotype of spl-1. However, further validation is needed to prove this function of the gene as well as its role in PCD, which in turn would be helpful to understand the mechanism and pathways involved in HR disease resistance of soybean.

show abstract

Inheritance of dlm and ti genes in Soybean

Cited by 3 publications

References 15 publications

Breeding of Black Soybean Line with ti and le Allele

Breeding of Black Soybean Line with ti and le Allele

Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon

Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)

Contact Info

Product

Resources

About