Breeding of Black Soybean Line with ti and le Allele

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. P=0.5-0.1). From eight F2 seeds with double recessive allele (ti/ti, le/le genotype), one new strain (titilele, both KTI, and lectin protein free) was developed. New strain has black seed coat, black hilum and green cotyledon in mature seed and 100-seed weight was 19.5 g. This strain will be helpful in breeding program for selecting black soybean line with lacking both KTI and lectin protein.

Breeding of tetra null soybean (Glycine max) for lipoxygenase, kunitz trypsin inhibitor, lectin, and 7S α' subunit proteins

“…Segregation ratios of independent inheritance between KTI protein and lectin protein were observed (χ 2 = 3.84). This result was consistent with previous studies that both Ti and Le alleles were independently inherited (Choi et al, 2016; Lee et al., 2008; Moraes et al., 2006; Orf & Hymowitz, 1979). Ti allele was independently inherited with Le allele in the F 2 population consisted of 24 plants (Moraes et al., 2006).…”

Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon

“…Also, a 3:1 segregation ratio was observed (χ 2 = 2.59, P=0.5 -0.1) for the presence or absence of lectin protein in the F 2 seed generation. This result substantiate previous observations that lectin protein is controlled by a single gene (Choi et al 2016;Sung et al 2013).…”

“…Between Le gene and Ti gene, segregation ratio of 9 : 3 : 3 : 1 (69 Le_Ti_: 32 lele Ti_: 22 Le_titi: 9 leletiti) was observed (χ 2 =2.87, P=0.5 -0.1) in the F 2 seed generation. This result agreed with previous papers that both Ti and Le alleles were independently inherited (Choi et al 2016;Lee et al 2008;Moraes et al 2006;. Independent inheritance of Ti and Le loci was observed in F 2 population consisted with 24 plants (Moraes et al 2006).…”

“…A 3:1 segregation ratio was observed (χ 2 =0.16, P=0.9 -0.5) for the presence or absence of KTI protein in the F 2 seed generation. Many researchers observed that the presence or absence of KTI protein is controlled by a single gene (Choi et al 2016;Eun et al 2012;Kim et al 2006;. Also, a 3:1 segregation ratio was observed (χ 2 = 2.59, P=0.5 -0.1) for the presence or absence of lectin protein in the F 2 seed generation.…”

“…Lee et al (2008) reported that Ti and Le alleles were independently inherited in 173 F 2 seed generation. Also, Choi et al (2016) observed that Le and Ti alleles were independently inherited in F 2 seed generation consisted with 179 seeds.…”