The phenolic composition, aroma compounds, physicochemical and antimicrobial properties of Nigella sativa L. (black cumin) honey

The aim of this study was to investigate the physicochemical properties, phenolic compounds, and antioxidant activities of eight chestnut honey samples collected from Artvin province in Turkiye. The honey samples' phenolic profiles were analyzed using reverse phase high performance liquid chromatography (RP-HPLC). All honeys were monofloral, and the quantities of chestnut sativa pollen ranged from 56 % to 80 %. Total phenolic and flavonoid contents and ferric reducing/antioxidant power (FRAP) values were assessed. The pH and moisture values of the honeys ranged between 4.60 and 5.40 and between 17.60 % and 19.00 %, respectively. Electrical conductivity (EC) and Hunter color L values ranged between 0.56 and 1.12 mS/cm and between 43.16 and 67.60, respectively. Proline values ranged from 876 to 1246 mg/kg. The diastase activities of all honeys were high, ranging between 18.28 and 26.30 DU. The mean of total phenolic, total flavonoid and total antioxidant (FRAP) levels in the samples were 72.79 � 0.03 mg GAE/100 g, 2.25 � 00.03mg QE/100 g, and 312.67 � 2.85 μmol FeSO 4 /100 g, respectively. Almost all of the 19 phenolic standards studied were detected in the samples, with catechin, chrysin, caffeic acid, caffeic acid phenethyl ester (CAPE) and gallic acid being determined as major components. In conclusion, the honeys from the Artvin region were high-quality chestnut honeys, with high polyphenolic contents and diversity and high apitherapeutic potentials.

Section: Resultsmentioning

confidence: 99%

Section: Phenolic Contents and Antioxidant Propertiesmentioning

confidence: 99%

An Investigation into Chestnut Honeys from Artvin Province in Turkiye: Their Physicochemical Properties, Phenolic Profiles and Antioxidant Activities

Saral

2023

“…For the evaluation of phenolic compounds, the chromatographic procedure previously mentioned in a study was followed [35] . Twenty‐five phenolic standards of gallic acid, protocatechuic acid, chlorogenic acid, p‐ OH benzoic acid, m ‐OH benzoic acid, ferulic acid, ellagic acid, caffeic acid, p‐ coumaric acid, syringic acid, epicatechin, rutin, daidzein, myricetin, luteolin, quercetin, resveratrol, apigenin, hesperidin, rhamnetin, chrysin, curcumin, pinocembrin, caffeic acid phenyl ester (CAPE), and t ‐cinnamic acid were defined qualitatively and quantitatively by considering their retention time and UV spectra in a Hitachi LaChrom Elite® HPLC system (Hitachi High – Technologies Corporation, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Comparison of Protective Effect of Aqueous and Ethanolic Extracts of Propolis against Gastric Ulceration in Rats

Şahin

Kaltalıoğlu

Erişgin³

et al. 2023

Self Cite

This study focused on the protective effects of different types of propolis extracts on gastric mucosa in indomethacininduced rats. The animals were divided into nine groups: control, negative control (ulcer), positive control (omeprazole), and experimental groups, which were summarized by 200, 400, and 600 mg/kg, bw for aqueous-based and ethanol, respectively. According to the histopathological evaluation, more than others, the doses of 200 and 400 mg/kg of aqueous-based propolis extracts had different degrees of positive effects on the gastric mucosa. Generally, the biochemical analyses of the gastric tissue showed a correlation with microscopic evaluations. According to the phenolic profile analysis, while pinocembrin (684.34 � 1.70 μg/ml) and chrysin (540.54 � 9.06 μg/ml) were the most abundant phenolics in the ethanolic extract, ferulic acid (53.77 � 0.07 μg/ml) and pcoumaric acid (52.61 � 0.42 μg/ml) dominated the aqueousbased extract. Also, the total phenolic content (TPC), total flavonoid content (TFC), and DPPH radical scavenging activity of the ethanolic extract showed almost nine-fold superiority compared to the aqueous-based extracts. Based on data from preclinical data, it was decided that the best doses for the main goal of the study were 200 mg and 400 mg/kg, bw for aqueous-based propolis extract.

“…[1,2] Honey, pollen, propolis, bee bread, royal jelly, and bee venom are natural bee products and which are important agents used in the complementary medicine known asapitherapy. [3,4] Propolis is a sticky product used in in the protection of the hive and consists of resins collected from plants by honeybees (Apis mellifera). [5,6] Bee pollen is a collection of the male reproductive cells of flowering plants and contains high levels protein, sugar, fat, vitamins, and polyphenols.…”

Section: Introductionmentioning

confidence: 99%

“…They have also been used therapeutically and for prophylactic purposes and to protect human health [1,2] . Honey, pollen, propolis, bee bread, royal jelly, and bee venom are natural bee products and which are important agents used in the complementary medicine known asapitherapy [3,4] . Propolis is a sticky product used in in the protection of the hive and consists of resins collected from plants by honeybees (Apis mellifera) [5,6] .…”

Section: Introductionmentioning

confidence: 99%

In Vitro Determination of Nitric Oxide Synthase Inhibition, Antioxidant Capacity and Phenolic Content of Various Natural Products (Bee and Herbal Products)

Yaylaci Karahalil,

Bakirhan,

Kara

2024

Self Cite

It is obvious that the oxidation process is an undeniable fact and when it comes to aging, one of the first solutions that come to mind is natural products. When it comes to natural products, both plants and bee products play an important, almost combative role against oxidation. For this purpose, natural products of both plant and animal origin were considered together in our study: Linden, green tea, aronia, wild grapes, myrtle, blueberries and basil, honey, pollen and propolis. Total phenolic content values of the extracts ranged between 49.28 and 3859.06 mg gallic acid equivalent/100 g, and propolis, green tea, chestnut flower and aronia samples were found to have the highest values. When looking at the NOS inhibition potential, it was determined that propolis, pollen and aronia samples had the highest percentage inhibition values of 98.11, 92.29, 83.44, respectively. Antioxidant activities of methanolic extracts were investigated using iron(III) reducing/antioxidant capacity (FRAP), 2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activity test and NOS inhibition tests. The phenolic composition of methanolic extracts was tested using the RP‐HPLC‐UV (high‐performance liquid chromatographic method with ultraviolet) method with 19 phenolic standards.