The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans

Abstract

Key messagePh-3is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein.AbstractLate blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-014-2303-1) contains supplementary material, which is available to authorized users.

MiR1918 enhances tomato sensitivity to Phytophthora infestans infection

“…However, field-grown tomatoes are susceptible to disease such as late blight (LB), which is one of the most devastating diseases of field-grown tomatoes, and it can cause severe economic losses for tomato growers242526. Late blight is caused by the pathogen Phytophthora infestans .…”

Molecular Markers Associated with <i>Ph</i>-3 Gene Conferring Late Blight Resistance in Tomato

“…This is potentially within the fine-map and clonned distance of the Ph-3 gene [7] [8]. Furthermore, we utilized the annotation information including CC-NBS-LRR to select the genomic sequence to design the primers (see Materials and Methods), which was the protein found in characterizing the clonned Ph-3 gene [8]. A strong association between genotypic and phenotypic data obtained from greenhouse experiments using field isolate was found in the present experiment.…”

“…With respect to previously identified RFLP molecular marker, TG591 associated with Ph-3, distance between TG591 and these molecular markers associated with Ph-3 are within 8 to 83 kbp distance (<1 cM) in this study (http://solgenomics.net/marker/SGN-M88/details),which is within the genome sequence of Ph-3 gene (Figure 2). This is potentially within the fine-map and clonned distance of the Ph-3 gene [7] [8]. Furthermore, we utilized the annotation information including CC-NBS-LRR to select the genomic sequence to design the primers (see Materials and Methods), which was the protein found in characterizing the clonned Ph-3 gene [8].…”

“…Recently, this gene was fine-mapped in the 0.5 cM genomic region of long arm of chromosome 9 in between Indel_3 and P55 molecular markers [7]. Utilizing the fine-map information, and considering the significance of the gene in late blight resistance, it was further characterized by cloning a 24 kb region [8]. Recently, co-dominant sequence characterized amplified region (SCAR) [9] and cleaved amplified polymorphic sequence (CAPS) markers have been developed for use in marker-assisted breeding for Ph-3 (M. Mutschler, pers.…”

Molecular Markers for Selecting Diverse Disease Resistances in Tomato Breeding Programs

“…Another resistance gene from S. pimpinellifolium "L3708", Ph3, which is the most effective LB resistance gene among the known genetic sources, confers incomplete resistance against a wide range of P. infestans isolates (Black et al 1996;Kim and Mutschler 2006;Zhang et al 2013). The Ph3 gene on chromosome 9 encodes a coiled-coil nucleotide-binding (NBS)-LRR (Zhang et al 2014). Ph4 on chromosome 2 was discovered in S. habrochaites LA1033 ( Kole et al 2006), and Ph5-1 and Ph5-2, which were discovered from a novel resistance source S. pimpinellifolium "PSLP153", are located on chromosome 1 and 10, respectively .…”