Molecular Markers for Selecting Diverse Disease Resistances in Tomato Breeding Programs

Lee, Je Min; Oh, Chang‐Sik; Yeam, Inhwa

doi:10.9787/pbb.2015.3.4.308

Cited by 28 publications

(25 citation statements)

References 109 publications

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“…TYLCV transmitted by the whitefly (B. tabaci) is one of the major challenges facing commercial tomato production worldwide. In Korea, TYLCV incidence was first reported in Tongyoung, Gyoengsangnam-do in 2008, and TYLCV has been transmitted rapidly all over the country (Lee et al 2015). Therefore, developing TYLCV-resistant cultivars is an urgent breeding goal for seed companies to maintain stable tomato production in Korea.…”

Section: Discussionmentioning

confidence: 99%

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-3

Dong

Han²,

Siddique³

et al. 2016

Plant Breed. Biotech.

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The viral disease induced by Tomato yellow leaf curl virus (TYLCV) reduces tomato (Solanum lycopersicum) yield significantly in tropical and subtropical regions. A number of loci, including Ty-1 to Ty-5, conferring resistance to TYLCV have been described and introgressed into modern tomato cultivars. The availability of molecular markers linked to these genes would expedite the introgression of TYLCV resistance into commercial cultivars. In the present study, we developed gene-based markers linked to the Ty-3 gene using a segregating population derived from a cross between the TYLCV-resistant line S. lycopersicum 'A45' and the susceptible line S. lycopersicum 'A39'. Agrobacterium-mediated screening was used to test TYLCV resistance of plants in the segregating population, and the resistance was evaluated by a visual scoring method and polymerase chain reaction analysis. By comparing sequences of the Ty-3 genes of the resistant and susceptible lines, two high-resolution melting (HRM) markers (Ty3-HRM1 and Ty3-HRM2) and one sequence characterized amplified region (SCAR) marker (Ty3-SCAR1) were developed. The HRM markers were based on single nucleotide polymorphisms at the 13th exon and the 15th intron, whereas the SCAR marker was based on a 246-bp deletion in the 16th intron. These gene-based markers will be useful tools for marker-assisted selection in breeding programs to improve TYLCV resistance of tomato.

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Section: Discussionmentioning

confidence: 99%

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-3

Dong

Han²,

Siddique³

et al. 2016

Plant Breed. Biotech.

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“…Gene-based molecular markers of Ty-3 would be useful to predict the phenotypic variations. There have been gene-based markers developed for Ty-1/Ty-3 by comparative analysis of tomato accessions (Jung et al 2015;Lee et al 2015). In the present study, we developed Ty-3 gene-based co-dominant markers in a bi-parental population to improve the efficiency of genotyping and to increase the diversity of molecular markers.…”

Section: Introductionmentioning

confidence: 99%

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance GeneTy-3

Dong¹,

Han²,

Siddique³

et al. 2016

Plant Breed. Biotech

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2016 (February) ABSTRACT The viral disease induced by Tomato yellow leaf curl virus (TYLCV) reduces tomato (Solanum lycopersicum) yield significantly in tropical and subtropical regions. A number of loci, including Ty-1 to Ty-5, conferring resistance to TYLCV have been described and introgressed into modern tomato cultivars. The availability of molecular markers linked to these genes would expedite the introgression of TYLCV resistance into commercial cultivars. In the present study, we developed gene-based markers linked to the Ty-3 gene using a segregating population derived from a cross between the TYLCV-resistant line S. lycopersicum 'A45' and the susceptible line S. lycopersicum 'A39'. Agrobacterium-mediated screening was used to test TYLCV resistance of plants in the segregating population, and the resistance was evaluated by a visual scoring method and polymerase chain reaction analysis. By comparing sequences of the Ty-3 genes of the resistant and susceptible lines, two high-resolution melting (HRM) markers (Ty3-HRM1 and Ty3-HRM2) and one sequence characterized amplified region (SCAR) marker (Ty3-SCAR1) were developed. The HRM markers were based on single nucleotide polymorphisms at the 13th exon and the 15th intron, whereas the SCAR marker was based on a 246-bp deletion in the 16th intron. These gene-based markers will be useful tools for marker-assisted selection in breeding programs to improve TYLCV resistance of tomato.

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“…Interestingly, 17 of the 35 different mapped pathogen resistance genes (R gene) have been cloned successfully [6]. Wild relatives' tomato species: S. pimpinellifolium, S. pennellii, S. habrochaites, S. peruvianum, and S. chilense constitute the main sources of resistance for R genes that have been introgressed into cultivated tomato [7,8].…”

Section: Introductionmentioning

confidence: 99%

Validation of some disease-resistance molecular markers associated with multiple diseases in tomato for marker-assisted selection program

Nevame¹,

Xia²,

Zhang³

et al. 2020

ScienceAsia

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Marker-assisted selection (MAS) is a tool that is widely applied in tomato resistance breeding. To determine the robustness of some molecular markers commonly used in MAS, extensive screening of 964 tomato lines was performed under a controlled experimental condition. Initially, the application of 36 molecular markers targeting 26 resistance genes (R genes) and 14 major diseases was evaluated. Here, we employed basic molecular biology and bioinformatics techniques for analysis where polymorphism, accuracy and clearness of amplicons constituted the selection criteria of markers. Upon initial analysis, 20 of these markers designated as efficient markers, among which 8 were considered gene-based markers and referred to as perfect markers were selected for detail evaluation. Information extrapolated from PCR result revealed 18 R genes that control 12 diseases were grouped under efficient markers. On the other hand, grouping of breeding lines based on the number of R gene harbored comprehensively revealed 62% of the lines to be void of R gene, while 38% carry different types of R genes. This provides us with an avenue to better understand new sources of resistance in the breeding lines. Conclusively, these efficient markers and their limited PCR condition can be suggested as basis of a diagnostic kit for MAS applications against 12 major tomato diseases and the identified resistant breeding lines could be conserved in order to be propagated as different sources of resistance for the development of new resistant varieties. Therefore, in areas with high vulnerability to diseases, high efficiency combination of the relevant R genes and their pyramiding into commercial tomato varieties are proposed to be implemented as a pragmatic approach.

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Molecular Markers for Selecting Diverse Disease Resistances in Tomato Breeding Programs

Cited by 28 publications

References 109 publications

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-3

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-3

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance GeneTy-3

Validation of some disease-resistance molecular markers associated with multiple diseases in tomato for marker-assisted selection program

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