Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-3

Abstract

The viral disease induced by Tomato yellow leaf curl virus (TYLCV) reduces tomato (Solanum lycopersicum) yield significantly in tropical and subtropical regions. A number of loci, including Ty-1 to Ty-5, conferring resistance to TYLCV have been described and introgressed into modern tomato cultivars. The availability of molecular markers linked to these genes would expedite the introgression of TYLCV resistance into commercial cultivars. In the present study, we developed gene-based markers linked to the Ty-3 gene using a segregating population derived from a cross between the TYLCV-resistant line S. lycopersicum 'A45' and the susceptible line S. lycopersicum 'A39'. Agrobacterium-mediated screening was used to test TYLCV resistance of plants in the segregating population, and the resistance was evaluated by a visual scoring method and polymerase chain reaction analysis. By comparing sequences of the Ty-3 genes of the resistant and susceptible lines, two high-resolution melting (HRM) markers (Ty3-HRM1 and Ty3-HRM2) and one sequence characterized amplified region (SCAR) marker (Ty3-SCAR1) were developed. The HRM markers were based on single nucleotide polymorphisms at the 13th exon and the 15th intron, whereas the SCAR marker was based on a 246-bp deletion in the 16th intron. These gene-based markers will be useful tools for marker-assisted selection in breeding programs to improve TYLCV resistance of tomato.

Hybrid analysis for Tolcv (Tomato Leaf Curl Virus) and Root Knot Nematode resistance in tomato (Solanum lycopersicum L.) And molecular validation of Ty-3 and Mi-1 gene

“…at 269 bp signi es the absence of the Ty-3 gene (Fig.1). This outcome aligns withDong et al (2016) andNevame et al (2018) ndings, where they employed the SCAR1 marker to assess segregating populations of the cross between S. lycopersicum -A45 (resistant) and S. lycopersicum -A39 (susceptible) for the presence of Ty-3 gene.The P6-25 marker yielded a solitary band of 630 bp in CBESL 159, CBESL 162, CBESL 164, CBESL 168, CBESL 169, H4, H5, H7, H8, H1xH7, H5xH7, H7xH5, H8xH5, and H4xH5, indicating the presence of the Ty-3 resistant allele. Conversely, the remaining thirteen parents and hybrids, such as CBESL 129, CBESL 133, CBESL 142, CBESL 143, CBESL 146, CBESL 154, CBESL 160, H1, H2, H3, H6, H1xH5, and H8xH7, displayed a single band at 320 bp, indicating the absence of the Ty-3 gene (Fig.2).…”

Validation of molecular markers for multiple disease resistance in tomato (Solanum lycopersicum)

“…1a). Dong et al (2016) designed gene based SCAR marker Ty-3 SCAR1 that could be effectively used for the selection of Ty-3 locus (ty-3/Ty-3 loci.). In our genotypes marker Ty-3 SCAR1 (Fig.…”

Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum

“…Introgression of resistance genes into cultivated varieties has been the principal route of breeding cultivars that are resistant to viruses such as Tomato yellow leaf virus (TYLCV), Tomato spotted wilt virus (TSWV), Pepper yellow leaf curl virus (PepYLCV) and Chilli veinal mottle virus (ChiVMV) [ 49 , 50 ]. The major markers linked to tomato yellow leaf disease (TYLCD) that have been developed to date include a closely linked molecular marker, SCAR1, for screening of the Ty-1 locus [ 51 ], P6-25 and FLUW25 for evaluation of Ty-3 , Ty-3a , and Ty-3b loci [ 37 , 50 , 52 ], and SCAR2 and P1-16 for the detection of Ty-2 [ 39 ]. Despite this progress, fewer markers for resistance to PepYLCV have been reported.…”