The petroleum-degrading bacteria Alcaligenes aquatilis strain YGD 2906 as a potential source of lipopeptide biosurfactant

Abstract

Soummam river sediments were used to isolate a biosurfactant-producing and petroleumdegrading bacterium. This strain was identified as Alcaligenes aquatilis YGD 2906 using phenotypic characterization and 16S ribosomal RNA sequencing. The culture supernatant of the isolated strain showed no haemolytic activity had an oiled displacement of 23.66 ± 0.57 mm and Emulsification index (E24) of 68.5% ± 0.5%. The biosurfactant produced in minimal medium was extracted by acid precipitation and quantified gravimetrically, with a yield of 4.2 ± 0.01 g / L. The crude Biosurfactant was determinate by TLC and MALDI-TOF-MS as a lipopeptide with protein and lipid content of8.49 ± 0.19% and 52.66 ± 1.16% respectively. This lipopeptide structure was confirmed by HPLC-MS/MS. This technique gave two main peak ranges which are typical of surfactins, iturins and fengycin. Tandem mass spectrometry was further used to elucidate the structure of the lipopeptide produced by the strain. The non-haemolytic nature of the lipopeptide produced by this strain offers opportunities for biomedical applications. Further work is needed to optimize production and analyze potential biomedical uses of this lipopeptide including antimicrobial properties.

An industrially potent rhamnolipid-like biosurfactant produced from a novel oil-degrading bacterium, Bacillus velezensis S2

“…[48][49][50][51] Moreover, biosurfactants derived from oil degrading bacteria are gaining strong status for both restoring environmental conditions as well as prospective commercial uses. [52][53][54][55] This study made use of potential oil degrading bacteria (ODB), isolated from oil contaminated soil samples collected from oil contaminated soil (15 years and 40 years old) and waste oil samples collected from MEGHNA Fatullah Depot, Narayanganj (23 0 33 0 -23 0 57 0 N and 90 0 26 0 -90 0 45 0 E), Bangladesh (Fig. 1A) for their application in petroleum remediation capability and production of industrially valuable biosurfactant.…”

Inoculation effect of Pseudomonas sp. TF716 on N2O emissions during rhizoremediation of diesel-contaminated soil

“…Aminobacter has been reported to degrade 2,6-dichlorobenzamide 78,79 . In addition, Shinella, Paracoccus, Azospirillum, Achromobacter, Alcaligenes, Bordetalla, Castenella, and Acidovorax are known to decompose TPHs 75,[80][81][82][83][84] . Although no statistical correlation was found, these bacterial groups are assumed to be involved in TPH degradation and nitrogenous compound metabolism, including N 2 O production and mitigation.…”

Crude Oil Biodegradation Potential using Acinetobacter baumannii CYA20 and Bacillus subtilis CYA27 from the Bekasi Coast, Indonesia

“…These bacteria can utilize hydrocarbon compounds as a carbon and energy source for growth and they can survive in a crude oil-polluted environment. In addition, they can degrade the crude oil components and oxidize hydrocarbons to CO 2 and H 2 O, which are harmless to the environment (Fu et al 2020), and they can also use hydrocarbons as electron donors and produce biosurfactants (Yalaoui-Guellal et al 2021). This condition will reduce or eliminate crude oil pollutants (Yang et al 2020).…”