The persistence of <i>Botrytis allii</i> in field soil

Maude, R. B.; Presly, A. H.

doi:10.1111/j.1365-3059.1982.tb01275.x

Cited by 11 publications

(8 citation statements)

References 4 publications

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“…While symptoms develop during storage, infection takes place in the field [1]. Leaf tips are the primary infection court for B. aclada , from which the fungus starts to grow towards the onion neck [4,5] with primary inoculum being either seed‐borne [4,6] or soil‐borne [7]. Secondary spore production from necrotic leaf tips is epidemiologically important [4].…”

Section: Introductionmentioning

confidence: 99%

Biocontrol agents efficiently inhibit sporulation of Botrytis aclada on necrotic leaf tips but spread to adjacent living tissue is not prevented

Yohalem¹,

Nielsen²,

Green³

et al. 2004

FEMS Microbiology Ecology

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Ulocladium atrum (isolates 385 and 302) consistently inhibited Botrytis aclada sporulation on dead onion leaf pieces under constant moist conditions and with an interrupted wetness period of 9 h. Clonostachys rosea (isolate 201) was as effective as U. atrum under constant moist conditions, but was ineffective if exposed to a drying period. No sporulation of B. aclada was observed 8 and 12 days after inoculation in the presence of U. atrum 302. C. rosea 201 significantly reduced B. aclada sporulation 8 days, but not 12 days after inoculation. When U. atrum 302 or C. rosea 201 was applied 1 day prior to B. aclada the antagonistic effect was higher compared to when the antagonists were applied on the same day. C. rosea 201 and U. atrum 302 did not obstruct the growth of B. aclada from necrotic onion leaf tips into living tissue, when artificially induced necrotic leaf tips were infested with B. aclada 24 h prior to antagonists. Three days after antagonist application, no symptoms could be observed on the healthy leaf tissue, nor was there sporulation on the necrotic leaf tip. However, B. aclada was immunologically detected 2 cm below the inoculation site. We conclude that under constant moist conditions the antagonists C. rosea 201 and U. atrum 302 cannot stop the progress of B. aclada from necrotic into fresh leaf tissue.

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Section: Introductionmentioning

confidence: 99%

Biocontrol agents efficiently inhibit sporulation of Botrytis aclada on necrotic leaf tips but spread to adjacent living tissue is not prevented

Yohalem¹,

Nielsen²,

Green³

et al. 2004

FEMS Microbiology Ecology

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“…of inoculum in the surrounding area which could explain why the infection was detected earlier on onion plants during both sampling years. Other studies also confirmed that the conidia produced during the previous season can infect plants at the seedling stage (Maude and Presly 1977;Maude et al 1982;du Toit et al 2004), resulting in asymptomatic plants, which can develop symptoms of neck rot during storage (Maude 1990). Despite the seasonal variations in the temperature, relative humidity, and amount of precipitation that the Portage La Prairie area received during 2019 and 2020 (Figs.…”

Section: Discussionmentioning

confidence: 77%

Early detection of onion neck rot disease in Manitoba

et al. 2021

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Neck rot caused by the fungus Botrytis allii Munn. is one of the most devastating diseases of onions (Allium cepa L.) resulting in significant yield losses. This disease is latent in nature developing symptomless onion plants in the field with bulbs typically showing symptoms 1-2 months after harvest in the storage. Molecular studies were conducted to detect latent infections of Botrytis neck rot in the onion fields of Manitoba, Canada. Plant samples of onion cultivars Redwing and Pocono were collected every 10 days throughout the growing season starting from planting until bulb harvesting during 2018, 2019, and 2020 from a research farm in Brandon, Manitoba, and plant samples of cultivar Redwing were collected during 2019 and 2020 from a commercial vegetable farm in Portage La Prairie, Manitoba. The amplified DNA fragment of onion leaves and neck region of collected samples were subjected to polymerase chain reaction (PCR) using Botrytis specific primer pair BA2f/BA1r. Botrytis allii was detected on onion samples collected from the commercial farm as early as the end of June 2019 and 2020 when plants were at the 5-7 leaf stage. The majority of onion samples collected from the research farm also started testing positive for the pathogen from June (2019, 2020) and July (2018) onwards. This knowledge about the timing of infection in the field will be useful in helping farmers to develop and evaluate management strategies in the field, and also predict the storability and availability of quality bulbs for sale.

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“…Although the open wound resulting from the removal of foliage at harvest is an important site for bulb infection, many plants are infected early in the growing season without displaying visible disease symptoms (Maude, 1990). Conidia produced during the season can infect plants at the seedling stage (du Toit, et al, 2004;Maude et al, 1982;Maude and Presly, 1977), resulting in asymptomatic plants, which can develop symptoms of neck rot during storage (Maude, 1990). The ability to quantify the level of inoculum in bulbs at different sampling times either during growth or during storage could yield insight into the growth patterns or virulence of the pathogen.…”

Section: Discussionmentioning

confidence: 99%

A Quantitative Real-time Polymerase Chain Reaction Assay for Botrytis aclada in Onion Bulb Tissue

Coolong¹,

Walcott²,

Randle³

2008

horts

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A real-time polymerase chain reaction (PCR) assay has been developed for the detection and quantification of Botrytis aclada (Fresenius), a causal agent of neck rot in onion (Allium cepa L.) bulbs. The assay uses TaqMan probe-based chemistry to detect an amplicon from the L45-550 region of B. aclada while using a DNA sequence from the onion serine acetyl transferase gene (SAT1) as a control. The assay detection limits for B. aclada and onion were 10 pg·μL−1 of genomic DNA. The detection limit for lyophilized B. aclada mycelium was 1 μg. The presence of onion tissue in the samples did not affect the performance of the real-time PCR assay. The assay distinguished among different amounts of B. aclada mycelium growing on onion disks that were inoculated with 0, 102, or 104 B. aclada conidia. Visual observations during the incubation period corresponded with changes in real-time PCR results. This assay could be used to determine the amount of B. aclada mycelium in bulbs during growth, harvest, and storage, thus giving researchers an objective and efficient tool by which to quantify the growth rate and virulence of B. aclada strains in vivo.

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The persistence of Botrytis allii in field soil

Cited by 11 publications

References 4 publications

Biocontrol agents efficiently inhibit sporulation of Botrytis aclada on necrotic leaf tips but spread to adjacent living tissue is not prevented

Biocontrol agents efficiently inhibit sporulation of Botrytis aclada on necrotic leaf tips but spread to adjacent living tissue is not prevented

Early detection of onion neck rot disease in Manitoba

A Quantitative Real-time Polymerase Chain Reaction Assay for Botrytis aclada in Onion Bulb Tissue

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