The peroxidase-thiocyanate-hydrogenperoxide system in tear fluid and saliva of different species

Haeringen, N.J. van; Ensink, F.T.E.; Glasius, E.

doi:10.1016/0014-4835(79)90095-2

Cited by 47 publications

(24 citation statements)

References 14 publications

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“…In vivo, the SPO and LPO systems essentially use only the pseudohalide SCN -as a substrate to produce OSCN - (Pruitt et al, 1988). Such defensive peroxidases are commonly found in regions of the human body that are controlled by the mucosa: e.g., breast milk (Shin et al, 2000), lachrymal fluid ( Van Haeringen et al, 1979;Tenovuo et al, 1985), and the mucosal lining of the lungs (Gerson et al, 2000). LPO and SPO are coded for the same gene (Ueda et al, 1997).…”

Section: Introduction Tmentioning

confidence: 99%

Inorganic Chemistry of Defensive Peroxidases in the Human Oral Cavity

Ashby

2008

J Dent Res

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The innate host response system is comprised of various mechanisms for orchestrating host response to microbial infection of the oral cavity. The heterogeneity of the oral cavity and the associated microenvironments that are produced give rise to different chemistries that affect the innate defense system. One focus of this review is on how these spatial differences influence the two major defensive peroxidases of the oral cavity, salivary peroxidase (SPO) and myeloperoxidase (MPO). With hydrogen peroxide (H2O2) as an oxidant, the defensive peroxidases use inorganic ions to produce antimicrobials that are generally more effective than H2O2 itself. The concentrations of the inorganic substrates are different in saliva vs. gingival crevicular fluid (GCF). Thus, in the supragingival regime, SPO and MPO work in unison for the exclusive production of hypothiocyanite (OSCN−, a reactive inorganic species), which constantly bathes nascent plaques. In contrast, MPO is introduced to the GCF during inflammatory response, and in that environment it is capable of producing hypochlorite (OCl−), a chemically more powerful oxidant that is implicated in host tissue damage. A second focus of this review is on inter-person variation that may contribute to different peroxidase function. Many of these differences are attributed to dietary or smoking practices that alter the concentrations of relevant inorganic species in the oral cavity ( e.g.: fluoride, F−; cyanide, CN−; cyanate, OCN−; thiocyanate, SCN−; and nitrate, NO3−). Because of the complexity of the host and microflora biology and the associated chemistry, it is difficult to establish the significance of the human peroxidase systems during the pathogenesis of oral diseases. The problem is particularly complex with respect to the gingival sulcus and periodontal pockets (where the very different defensive stratagems of GCF and saliva co-mingle). Despite this complexity, intriguing in vitro and in vivo studies are reviewed here that reveal the interplay between peroxidase function and associated inorganic chemistry.

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Section: Introduction Tmentioning

confidence: 99%

Inorganic Chemistry of Defensive Peroxidases in the Human Oral Cavity

Ashby

2008

J Dent Res

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“…Unstimulated tear samples of 1 Ìl were collected, either from the left or right eye, in 5-Ìl glass capillaries and diluted in 50 mM Tris-HCl buffer, pH 7.0, in a ratio of 1:75. The glass micropipette was gently pushed into the conjunctival sac to avoid ocular surface contact, according to previous reports [1,9]. All the samples were immediately stored at -20°C until processing.…”

Section: Sample Collectionsmentioning

confidence: 99%

“…Peroxidases are widely distributed in nature and their functional role has been supposed to be involved in the control of bacterial flora and oxidative tissue damage [1,2]. In mammals, tissues displaying growth dependency on oestrogen (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…uterus, cervix, vagina, mammary gland and rat induced mammary tumour) synthesise and secrete endogenous peroxidases, which are also considered an important marker of oestrogen-regulated proteins [3,4]. Peroxidases are mainly located in exocrine secretions such as saliva, milk and tears [1]. For the first two secretions, a control of peroxidase activity by oestrogen has been shown [3,5], whereas for lacrimal peroxidase only data indicating a dependency on the autonomic nervous system are available [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Variations of Lacrimal Fluid Peroxidase Activity in Female and Male Rats

Madia

Liberati²,

Feo³

et al. 2001

Ophthalmic Res

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Previous research from this laboratory showed that human lacrimal fluid peroxidase has cyclic variations during the menstrual cycle, correlated with plasma levels of 17β-oestradiol. In the present investigation, variations of enzyme activity and total protein content during the oestrous cycle of young adult female rats are analysed. Effects from circadian rhythm and a gender-related influence are also examined. In female rats, as in women, lacrimal fluid peroxidase activity shows cyclic variations; in fact, it significantly (p < 0.05) changes during the different phases of the oestrous cycle. In contrast, in males such variations do not occur. Thus, we suggest that gender seems to exert a significant influence on the secretion of this specific tear protein, probably by a direct effect of oestrogens.

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“…Tais peroxidases defensivas são comumente encontradas nas mucosas, leite materno (Shin et al, 2000), fluido lacrimal (Van Haeringen et al, 1979;Tenovuo et al, 1985), e nas mucosas terminais dos pulmões (Gerson et al, 2000). LPO e POS são codificadas pelo mesmo gene (Ueda et al, 1997).…”

Section: Peroxidase Salivar (Origem E Função)unclassified

Efeito do tratamento periodontal não cirúrgico na atividade da peroxidase na saliva da glândula parótida e no fluído gengival

Gonçalves¹

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AGRADECIMENTOSAo meu orientador, Prof. Titular Francisco Emílio Pustiglioni, pela confiança, incentivo, ensinamentos e paciência. Foi uma longa convivência, na qual pude aprender que nossos objetivos são apenas alcançados quando realmente vamos atrás, nada vem de graça.Ao Prof. Dr. Marcelo Nicolás Muscará, pela co-orientação e por ceder seu laboratório para a realização dos experimentos desta pesquisa. CONCLUSIONS: In periodontal disease MPO/GCF presented higher levels than controls and MPO/GCF and POS parotid are reduced after treatment.

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The peroxidase-thiocyanate-hydrogenperoxide system in tear fluid and saliva of different species

Cited by 47 publications

References 14 publications

Inorganic Chemistry of Defensive Peroxidases in the Human Oral Cavity

Inorganic Chemistry of Defensive Peroxidases in the Human Oral Cavity

Variations of Lacrimal Fluid Peroxidase Activity in Female and Male Rats

Efeito do tratamento periodontal não cirúrgico na atividade da peroxidase na saliva da glândula parótida e no fluído gengival

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