The perivascular phenotype and behaviors of dedifferentiated cells derived from human mature adipocytes

Song, Ning; Kou, Liang; Lu, Xionggang; Sugawara, Atsunori; Shimizu, Yutaka; Wu, Min-Ke; Du, Li; Wang, Hang; Sato, Soh; Shen, Jiefei

doi:10.1016/j.bbrc.2015.01.033

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…10 Mature adipocytes were observed under light microscope to break into small lipid droplets, loose their round contour and fibroblast --like morphology is obtained after 10 days. 14,29 Some other studies showed that during dedifferentiation process, mature adipocytes divide asymmetrically and give rise to another adipocyte as well as a non--adipocyte daughter cell that further produce the DFAT cells. Matsumoto et al showed that DNA synthesis in the mature adipocyte is induced by the ceiling culture by BrdU incorporation method.…”

Section: Phenotypic Properties Of Dfat Cellsmentioning

confidence: 98%

Current challenges in dedifferentiated fat cells research

et al. 2016

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Dedifferentiated fat cells show great promises as a novel cell source for stem cell research. It has many advantages when used for cell-based therapeutics including abundance, pluripotency, and safety. However, there are many obstacles researchers need to overcome to make the next big move in DFAT cells research. In this review, we summarize the current main challenges in DFAT cells research including cell culture purity, phenotypic properties, and dedifferentiation mechanisms. The common methods to produce DFAT cells as well as the cell purity issue during DFAT cell production have been introduced. Current approaches to improve DFAT cell purity have been discussed. The phenotypic profile of DFAT cells have been listed and compared with other stem cells. Further studies on elucidating the underlying dedifferentiation mechanisms will dramatically advance DFAT cell research.

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Section: Phenotypic Properties Of Dfat Cellsmentioning

confidence: 98%

Current challenges in dedifferentiated fat cells research

et al. 2016

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“…White adipocytes may be enzymatically dissociated from adipose tissues, but they are difficult to isolate efficiently because of their large size (50 to 200 μm) and tendency to lyse rapidly during manipulation or brief storage [ 17 ]. Fluorescence activated cell sorting (FACS) has been used to fractionate dissociated adipocytes, but the large cell sizes require special instrumentation to prevent clogging and slow flow rates [ 18 ]. Furthermore, very few immunochemical surface markers are available that distinguish subsets of adipocytes.…”

Section: Introductionmentioning

confidence: 99%

Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine

Lee

et al. 2016

PLoS ONE

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The reprogramming of cellular memory in specific cell types, and in visceral adipocytes in particular, appears to be a fundamental aspect of obesity and its related negative health outcomes. We explored the hypothesis that adipose tissue contains epigenetically distinct subpopulations of adipocytes that are differentially potentiated to record cellular memories of their environment. Adipocytes are large, fragile, and technically difficult to efficiently isolate and fractionate. We developed fluorescence nuclear cytometry (FNC) and fluorescence activated nuclear sorting (FANS) of cellular nuclei from visceral adipose tissue (VAT) using the levels of the pan-adipocyte protein, peroxisome proliferator-activated receptor gamma-2 (PPARg2), to distinguish classes of PPARg2-Positive (PPARg2-Pos) adipocyte nuclei from PPARg2-Negative (PPARg2-Neg) leukocyte and endothelial cell nuclei. PPARg2-Pos nuclei were 10-fold enriched for most adipocyte marker transcripts relative to PPARg2-Neg nuclei. PPARg2-Pos nuclei showed 2- to 50-fold higher levels of transcripts encoding most of the chromatin-remodeling factors assayed, which regulate the methylation of histones and DNA cytosine (e.g., DNMT1, TET1, TET2, KDM4A, KMT2C, SETDB1, PAXIP1, ARID1A, JMJD6, CARM1, and PRMT5). PPARg2-Pos nuclei were large with decondensed chromatin. TAB-seq demonstrated 5-hydroxymethylcytosine (5hmC) levels were remarkably dynamic in gene bodies of various classes of VAT nuclei, dropping 3.8-fold from the highest quintile of expressed genes to the lowest. In short, VAT-derived adipocytes appear to be more actively remodeling their chromatin than non-adipocytes.

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Dedifferentiation and Adipose Tissue

Zhao

2018

Cellular Dedifferentiation and Regenerative Medicine

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The perivascular phenotype and behaviors of dedifferentiated cells derived from human mature adipocytes

Cited by 5 publications

References 38 publications

Current challenges in dedifferentiated fat cells research

Current challenges in dedifferentiated fat cells research

Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine

Dedifferentiation and Adipose Tissue

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