The performance of poly-ε-caprolactone scaffolds in a rabbit femur model with and without autologous stromal cells and BMP4

Savarino, L.; Baldini, Nicola; Greco, Margherita; Capitani, Ombretta; Pinna, Stefania; Valentini, Serena; Lombardo, Barbara; Esposito, Marco; Pastore, Lucio; Ambrosio, Luigi; Battista, Sabrina; Causa, Filippo; Zeppetelli, S.; Guarino, Vincenzo; Netti, Paolo A.

doi:10.1016/j.biomaterials.2007.03.011

Cited by 63 publications

(44 citation statements)

References 23 publications

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“…21 Briefly, a biomaterial scaffold suitable for use in tissue engineering should be biodegradable and have nontoxic degradation products, be highly porous with an interconnected pore structure, have suitable physical and mechanical properties, and be biocompatible and suitable for cell attachment, proliferation, and differentiation. 22 Various methods have been investigated for the preparation of porous scaffolds, including electrospinning, 23 solvent casting/ particulate leaching, 24 phase inversion, 25 freeze-drying, 26 and thermally-induced phase separation. 27 The solvent casting/salt leaching method has the advantage of controlling pore size by manipulating the size of the salt particulate.…”

Section: Introductionmentioning

confidence: 99%

“…Researchers have used combinations of these techniques to obtain desirable porosity ratios and pore dimensions. [25][26][27] In this study, biodegradable linear polyurethanes were prepared using polycaprolactone, HMDI, and 1,4-butanediol/HMDI/1,4-butanediol (BDO/ HMDI/BDO) copolymers. Scaffolds with interconnected porosity were prepared using a combination of salt leaching and freeze-drying methods.…”

Section: Introductionmentioning

confidence: 99%

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Manufacturing of biodegradable polyurethane scaffolds based on polycaprolactone using a phase separation method: physical properties and in vitro assay

Asefnejad¹,

Khorasani²,

Behnamghader³

et al. 2011

IJN

164

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Background Biodegradable polyurethanes have found widespread use in soft tissue engineering due to their suitable mechanical properties and biocompatibility. Methods In this study, polyurethane samples were synthesized from polycaprolactone, hexamethylene diisocyanate, and a copolymer of 1,4-butanediol as a chain extender. Polyurethane scaffolds were fabricated by a combination of liquid–liquid phase separation and salt leaching techniques. The effect of the NCO:OH ratio on porosity content and pore morphology was investigated. Results Scanning electron micrographs demonstrated that the scaffolds had a regular distribution of interconnected pores, with pore diameters of 50–300 μm, and porosities of 64%–83%. It was observed that, by increasing the NCO:OH ratio, the average pore size, compressive strength, and compressive modulus increased. L929 fibroblast and chondrocytes were cultured on the scaffolds, and all samples exhibited suitable cell attachment and growth, with a high level of biocompatibility. Conclusion These biodegradable polyurethane scaffolds demonstrate potential for soft tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Manufacturing of biodegradable polyurethane scaffolds based on polycaprolactone using a phase separation method: physical properties and in vitro assay

Asefnejad¹,

Khorasani²,

Behnamghader³

et al. 2011

IJN

164

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“…A healthy left femur of a New Zealand rabbit was modelled by computer tomography (CT) using the MIMICS software (Materialise 2006; figure 1a). On this solid model, a virtual segmental 3!7 mm defect in the proximal third of the left femur, laterally to the greater trochanter (Savarino et al 2007), was performed in order to simulate the scaffold implantation. The FE mesh of that model was obtained using HARPOON software (Sharc 2006) with four-node bilinear tetrahedra in both the bone and scaffold macroscopic domains.…”

Section: Resultsmentioning

confidence: 99%

“…The mathematical model described above was mathematically implemented for an application example found in the literature (Savarino et al 2007). A healthy left femur of a New Zealand rabbit was modelled by computer tomography (CT) using the MIMICS software (Materialise 2006; figure 1a).…”

Section: Resultsmentioning

confidence: 99%

A mathematical approach to bone tissue engineering

Sanz-Herrera

García-Aznar

Doblaré

2009

Phil. Trans. R. Soc. A.

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Tissue engineering is becoming consolidated in the biomedical field as one of the most promising strategies in tissue repair and regenerative medicine. Within this discipline, bone tissue engineering involves the use of cell-loaded porous biomaterials, i.e. bioscaffolds, to promote bone tissue regeneration in bone defects or diseases such as osteoporosis, although it has not yet been incorporated into daily clinical practice. The overall success of a particular bone tissue engineering application depends strongly on scaffold design parameters, which do away with long and expensive clinical protocols. Computer simulation is a useful tool that may reduce animal experiments and help to identify optimal patient-specific designs after concise model validation. In this paper, we present a novel mathematical approach to bone regeneration within scaffolds, based on a multiscale framework. Results are presented over an actual scaffold microstructure, showing the potential of computer simulation, and how it can aid in the task of making bone tissue engineering a reality in clinical practice.

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“…Bone marrow mesenchymal stem cells (BMSCs) were isolated from the femoral bone shaft of New Zealand rabbits [18]. The bone marrow was washed out from the femoral medullary cavity and centrifuged at 800 r/min for ten minutes, the upper liquid was discarded, and the rest of the cells were suspended in Dulbecco's Modified Eagle's Medium (DMEM, 100 U/mL penicillin, 100 μg/mL streptomycin, 15% foetal bovine serum, 0.01 mol/L HEPES), then incubated at 37°C in 5% CO 2 .…”

Section: Preparation Of the Tissue-engineered Bonementioning

confidence: 99%

A new osteonecrosis animal model of the femoral head induced by microwave heating and repaired with tissue engineered bone

Han

Chen

et al. 2008

International Orthopaedics (SICOT)

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The objective of this research was to induce a new animal model of osteonecrosis of the femoral head (ONFH) by microwave heating and then repair with tissue engineered bone. The bilateral femoral heads of 84 rabbits were heated by microwave at various temperatures. Tissue engineered bone was used to repair the osteonecrosis of femoral heads induced by microwave heating. The roentgenographic and histological examinations were used to evaluate the results. The femoral heads heated at 55°C for ten minutes showed low density and cystic changes in X-ray photographs, osteonecrosis and repair occurred simultaneously in histology at four and eight weeks, and 69% femoral heads collapsed at 12 weeks. The ability of tissue engineered bone to repair the osteonecrosis was close to that of cancellous bone autograft. The new animal model of ONFH could be induced by microwave heating, and the tissue engineering technique will provide an effective treatment.Résumé L'objectif de cette étude est de constituer un modèle animal d'ostéonécrose de la tête fémorale (ONFH) par micro traumatismes et guérison par greffe. Matériel et méthode: les deux têtes fémorales de 84 lapins ont été soumises à des micro traumatismes à des températures variables. L'os a été utilisé pour la réparation de cette ostéonécrose. Un examen radiologique et histologique ont également été réalisés de façon à évaluer les résultats. Résultats: la tête fémorale traumatisée à 55 iàC pendant 10 minutes montre des modifications de densité osseuse avec apparition de kystes sur les radiographies avec une ostéonécrose et une réparation qui apparaît entre 4 et 8 semaines. 69% des têtes fémorales sont effondrées à 12 semaines. La réparation osseuse a été réalisée par de l'os spongieux. Conclusion: ce nouveau modèle animal permet de réaliser des micro traumatismes et montre que la réparation cellulaire peut être réalisée de façon effective.

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The performance of poly-ε-caprolactone scaffolds in a rabbit femur model with and without autologous stromal cells and BMP4

Cited by 63 publications

References 23 publications

Manufacturing of biodegradable polyurethane scaffolds based on polycaprolactone using a phase separation method: physical properties and in vitro assay

Manufacturing of biodegradable polyurethane scaffolds based on polycaprolactone using a phase separation method: physical properties and in vitro assay

A mathematical approach to bone tissue engineering

A new osteonecrosis animal model of the femoral head induced by microwave heating and repaired with tissue engineered bone

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