The performance of laminin-containing cryogel scaffolds in neural tissue regeneration

Jurga, Marcin; Dainiak, Maria B.; Sarnowska, Anna; Jabłońska, Anna; Tripathi, Anuj; Plieva, Fatima M.; Savina, Irina N.; Strojek, Lukasz; Jungvid, Hans; Kumar, Ashok; Łukomska, Barbara; Domanska-Janik, Krystyna; Forraz, Nico; McGuckin, Colin

doi:10.1016/j.biomaterials.2011.01.049

Cited by 143 publications

(106 citation statements)

References 46 publications

Supporting

Mentioning

103

Contrasting

Order By: Relevance

“…However, the direct calculations of the PSD with quantitative analysis of SEM images were not carried out in this paper [80]. Additionally, Jurga et al [112] prepared bioactive scaffolds using cryogelation of dextran or gelatine linked to laminin. The textural characteristics of native cryogels were analysed using CLSM.…”

mentioning

confidence: 98%

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

Self Cite

266

205

View full text Add to dashboard Cite

*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

show abstract

mentioning

confidence: 98%

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

Self Cite

266

205

View full text Add to dashboard Cite

show abstract

“…Such pores are ideal for cell aggregates to adhere and develop a micro-niche. The pore size between 80-100 µm of dextran or gelatin cryogels was previously shown to be optimal for stem cells derived from human cord blood adhesion and neural differentiation [12]. Subsequently it was shown that gelatin-based cryogels scaffolds with the pore size ranging from 20 to 120 allow encapsulation of bigger in size mesenchymal stem cells (hUCMSCs) and when supplemented with neuromorphogenes promote their neural differentiation in vitro [32].…”

Section: Discussionmentioning

confidence: 99%

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Pietrucha¹,

Zychowicz²,

Podobińska³

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Jurga et al 51 created cryogels containing LM (0.01% w/v) and fish skin gelatin (4.4% w/v) that were crosslinked with glutaraldehyde for mechanical stability. In hippocampal organotypic cultures, the gelatin-LM cryogels showed intensive migration of NF200 + neuroblasts throughout the entire volume of the scaffold.…”

Section: Central Nervous System Regenerationmentioning

confidence: 99%

Laminin Enriched Scaffolds for Tissue Engineering Applications

Garg¹

2017

ATROA

View full text Add to dashboard Cite

Laminin (LM) is heterotrimeric large molecular weight glycoprotein that forms a crucial component of the basal lamina (or basement membrane) of most tissues. LMs are integral for cell adhesion, proliferation, survival, migration, and differentiation. Several studies have used a LM-coated 2D substrate for the culture and maintenance of stem cell phenotype in vitro. Recent studies have reported that LM can also be incorporated in 3D scaffolds for tissue engineering applications. This article illustrates the bioactivity and regenerative potential of LM protein in 3D scaffolds for skeletal muscle, nerve, vascular, and intervertebral-disc regeneration.

show abstract

The performance of laminin-containing cryogel scaffolds in neural tissue regeneration

Cited by 143 publications

References 46 publications

Cryogels: Morphological, structural and adsorption characterisation

Cryogels: Morphological, structural and adsorption characterisation

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Laminin Enriched Scaffolds for Tissue Engineering Applications

Contact Info

Product

Resources

About