Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Pietrucha, Krystyna; Zychowicz, Marzena; Podobińska, Martyna; Bużañska, Leonora

doi:10.5114/fn.2017.68578

Cited by 8 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8,16−23 As these materials all allow for diffusion of essential nutrients and morphogens throughout the tissue constructs, they can be used to maintain NPCs and neuronal cultures for extended studies of differentiation and maturation, including axon formation, growth, and pruning. 17,24,25 Additionally, these platforms have demonstrated utility for assessing disease phenotypes when the hPSCs are sourced from patients that harbor genetic risk factors for each disorder. 16,18 Despite their utility toward fabricating complex neural tissue structures from hPSCs, existing ECMs have some known shortcomings for neural cell culture.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Matrigel is also the predominant ECM utilized for more recently developed hPSC-derived brain organoids, , where the ECM scaffold supports the self-organization of the neuroepithelium to induce neuroepithelial buds and facilitates growth by providing a physical structure for the cells to attach and grow . Other natural and synthetic materials have been developed for extended culture of hPSC-derived neural progenitor cells, (NPCs), neurons, and organoids, including silk, collagen, gelatin, hyaluronic acid (HA), elastin-like peptides, and polyethylene glycol (PEG). ,− As these materials all allow for diffusion of essential nutrients and morphogens throughout the tissue constructs, they can be used to maintain NPCs and neuronal cultures for extended studies of differentiation and maturation, including axon formation, growth, and pruning. ,, Additionally, these platforms have demonstrated utility for assessing disease phenotypes when the hPSCs are sourced from patients that harbor genetic risk factors for each disorder. , …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks

O’Grady

Balotin

Bosworth

et al. 2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

In vitro models of the human central nervous system (CNS), particularly those derived from induced pluripotent stem cells (iPSCs), are becoming increasingly recognized as useful complements to animal models for studying neurological diseases and developing therapeutic strategies. However, many current 3D CNS models suffer from deficits that limit their research utility. In this work, we focused on improving the interactions between the extracellular matrix (ECM) and iPSC-derived neurons to support model development. The most common ECMs used to fabricate 3D CNS models often lack the necessary bioinstructive cues to drive iPSC-derived neurons to a mature and synaptically connected state. These ECMs are also typically difficult to pattern into complex structures due to their mechanical properties. To address these issues, we functionalized gelatin methacrylate (GelMA) with an N-cadherin extracellular peptide epitope to create a biomaterial termed GelMA-Cad. After photopolymerization, GelMA-Cad forms soft hydrogels (on the order of 2 kPa) that can maintain patterned architectures. The N-cadherin functionality promotes survival and maturation of single-cell suspensions of iPSC-derived glutamatergic neurons into synaptically connected networks as determined by viral tracing and electrophysiology. Immunostaining reveals a pronounced increase in presynaptic and postsynaptic marker expression in GelMA-Cad relative to Matrigel, as well as extensive co-localization of these

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks

O’Grady

Balotin

Bosworth

et al. 2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…TUBB3 and MAP2) and appearance of glial markers [10]. These changes are accompanied by the neurite outgrowth and a proliferation rate decrease [8, 10–13], while in the further differentiation process in the defined media—mature, functional neurons are obtained [9]. Thus, eNP and NP are the intermediate stages of neural development between NSC and mature neurons.…”

Section: Introductionmentioning

confidence: 99%

Reference Gene Validation via RT–qPCR for Human iPSC-Derived Neural Stem Cells and Neural Progenitors

et al. 2019

Self Cite

View full text Add to dashboard Cite

Correct selection of the reference gene(s) is the most important step in gene expression analysis. The aims of this study were to identify and evaluate the panel of possible reference genes in neural stem cells (NSC), early neural progenitors (eNP) and neural progenitors (NP) obtained from human-induced pluripotent stem cells (hiPSC). The stability of expression of genes commonly used as the reference in cells during neural differentiation is variable and does not meet the criteria for reference genes. In the present work, we evaluated the stability of expression of 16 candidate reference genes using the four most popular algorithms: the ΔCt method, BestKeeper, geNorm and NormFinder. All data were analysed using the online tool RefFinder to obtain a comprehensive ranking. Our results indicate that NormFinder is the best tool for reference gene selection in early stages of hiPSC neural differentiation. None of the 16 tested genes is suitable as reference gene for all three stages of development. We recommend using different genes (panel of genes) to normalise RT–qPCR data for each of the neural differentiation stages. Electronic supplementary material The online version of this article (10.1007/s12035-019-1538-x) contains supplementary material, which is available to authorized users.

show abstract

“…In the present study, three types of Col-based scaffolds were evaluated: Col sponges, bi-component Col-CS matrices crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and Col sponges modified using 2,3 DAC. It has been shown that binary systems of collagen-chondroitin sulphate (Col-CS) and collagen-dialdehyde cellulose (Col-DAC) have the correct balance of properties to serve as a biomimetic niche: They accommodate human induced pluripotent stem cells (hiPSC-NPs) sustaining their ability to proliferate and differentiate into neural lineages (12).…”

Section: Introductionmentioning

confidence: 99%

Intra‑cerebral implantation of a variety of collagenous scaffolds with nervous embryonic cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

Collagenous scaffolds provide good conditions for embryonic nerve cell growth. The aim of the current study was to assess the brains reaction to the implantation of 3D sponge-shaped scaffolds. These scaffolds consisted of collagen (Col) and Col with chondroitin sulphate, which is modified by carbodiimide, or Col crosslinked with dialdehyde cellulose. The current study also evaluated the expression of integrins α2 and β1 in embryonic nerve cells. Embryonic nerve cells were isolated from the brains of rat embryos. Acellular scaffolds, or scaffolds populated with embryonic nerve cells, were implanted into the rats brain. The fibers of all the implanted scaffolds remained intact and served as a template for cell infiltration. The implants induced minimal to moderate inflammatory responses and minimal glial scar formations. Immunohistochemical studies did not indicate any microtubule-associated protein 2 or glial fibrillary acidic protein-positive cells inside the scaffolds. Acellular and cell-populated scaffolds yielded similar responses in the brain. The expression of integrin α2 and β1 was observed in embryonic nervous cells. TC-I15, the integrin α2β1 inhibitor, was not demonstrated to modify cell entrapment within the collagenous scaffolds. All applied scaffolds were well tolerated by the tissue and were indicated to support blood vessel formation. Therefore, all tested biomaterials are recommended for further studies. Additional chemical modifications of the material are suggested to protect the seeded cells from apoptosis after implantation into the brain.

show abstract

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Abstract: A b s t r a c t The biomimetic, standardized conditions for in vitro cultures of human neural progenitors derived from induced pluripotent stem cells (hiPSC-NPs

Cited by 8 publications

References 39 publications

Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks

Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks

Reference Gene Validation via RT–qPCR for Human iPSC-Derived Neural Stem Cells and Neural Progenitors

Intra‑cerebral implantation of a variety of collagenous scaffolds with nervous embryonic cells

Contact Info

Product

Resources

About