The performance of Callosobruchus maculatus F. (Coleoptera: Bruchidae) in chitin containing artificial seeds

Moraes, R.A.; Sales, Maurı́cio P.; Xavier‐Filho, J.

doi:10.1590/s0301-80591998000200019

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Our results also showed that the AP2 and AP3 fractions consisted of fragments that bound even more strongly to chitin. However, these fractions were not tested against the insects because previous work showed that the acid solutions used to desorb chitin-bound proteins liberate chitin fragments that interfere with C. maculatus larvae development (22).…”

Section: Resultsmentioning

confidence: 99%

Toxicity of Hydrolyzed Vicilins toward Callosobruchus maculatus and Phytopathogenic Fungi

Uchôa

Miranda²,

Souza³

et al. 2009

J. Agric. Food Chem.

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Studies have shown that vicilins (7S storage proteins) from seeds were able to bind to the surface of the Callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of Diatraea saccharalis and Tenebrio molitor , inhibiting larval development. Vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. The present work studies the association of peptides from vicilins of genotypes of Vigna unguiculata (susceptible and resistant to bruchid) with acetylated chitin and the toxicity of vicilin fragments and chitin-binding vicilin fragments to C. maculatus and phytopathogenic fungi. Hydrolysis of vicilins with alpha-chymotrypsin results in a complex mixture of fragments that were separated by chitin-affinity chromatography. Chitin-binding peptides from both genotypes were toxic to C. maculatus larvae, and alpha-chymotrypsin-hydrolyzed vicilins were deleterious to the above insect and to Fusarium oxysporum , Colletotrichum musae , and Saccharomyces cerevisiae fungi.

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Section: Resultsmentioning

confidence: 99%

Toxicity of Hydrolyzed Vicilins toward Callosobruchus maculatus and Phytopathogenic Fungi

Uchôa

Miranda²,

Souza³

et al. 2009

J. Agric. Food Chem.

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“…Apesar de algumas pesquisas indicarem que a resistência a C. maculatus em caupi estava associada a alta concentração de inibidores de tripsina e/ou de α-amilase (Gatehouse et al 1979, Piergiovanni et al 1991, Baker et al (1989) e Zhu et al (1994) não encontraram correlação entre parâmetros biológicos e esses inibidores. Outros fatores, como uma variante da proteína vicilina, o polissacarídeo quitina, características morfológicas, além da combinação de características físicas e químicas, também estão envolvidos nessa resistência (Kitch et al 1991, XavierFilho 1991, Xavier-Filho et al 1996, Oingiangbe & Onigbinde 1996, Moraes et al 1998. Entretanto, a inconsistência das informações exige estudos adicionais para o isolamento dos fatores associados com a resistência de alguns grãos a C. maculatus.…”

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Identificação de genótipos de caupi Vigna unguiculata (L.) Walp. resistentes a Callosobruchus maculatus (Fabr.) (Coleoptera: Bruchidae)

Lima¹,

Oliveira²,

Barros³

et al. 2001

Neotrop. Entomol.

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-The present work aimed to identify sources of resistance in 30 cowpea genotypes, from EMBRAPA Meio-Norte, to the cowpea weevil Callosobruchus maculatus (Fabr.). In a no choice test, two pairs of the insect were confined in 30 grains of each genotype, in a completely randomized design with five replications. To evaluate the resistance of the genotypes, the parameters: number of eggs/ female, viability of eggs, duration and viability of the immature phase, natural rate of population increase, and resistance index were used. By this index the genotypes IT89KD-245, TE90-180-10F, CNCx 409-12F, IT86D-716, IT89KD-260 and BR17-Gurguéia were classified as moderately resistant; CNC 0434, CNCx 405-17F, TE90-170-76F, TVu-249, IT89KD-381, BR14-Mulato, TE87-98-8G, TE87-1115-10G, CNCx 955-1F, CNCx 676-51F, TE87-98-13G, TE90-172-42E and TE87-98-9G-2 as susceptible, and Bico de Pato, TE90-180-3E, TE90-179-14E, TE90-170-29F, Monteiro, TE84-27-7G, CNCx 409-11F, TE90-172-33F, TE90-180-88E, TE90-170-40F and TE90-180-13E as highly susceptible. The genotypes IT89KD-245 and CNCx 955-1F showed non-preference for oviposition. The genotype IT89KD-245 was the only one classified as resistant, showing the least natural rate of population increase, the smallest viability and the largest duration of the immature phase, expressing antibiosis.

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The performance of Callosobruchus maculatus F. (Coleoptera: Bruchidae) in chitin containing artificial seeds

Cited by 2 publications

References 6 publications

Toxicity of Hydrolyzed Vicilins toward Callosobruchus maculatus and Phytopathogenic Fungi

Toxicity of Hydrolyzed Vicilins toward Callosobruchus maculatus and Phytopathogenic Fungi

Identificação de genótipos de caupi Vigna unguiculata (L.) Walp. resistentes a Callosobruchus maculatus (Fabr.) (Coleoptera: Bruchidae)

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