The pea ferredoxin I gene exhibits different light responses in pea and tobacco.

Gallo‐Meagher, Maria; Sowinski, Dolores A.; Thompson, William F.

doi:10.1105/tpc.4.4.383

Cited by 13 publications

(6 citation statements)

References 15 publications

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“…Evidence for this has been obtained from studies with transgenic tobacco plants containing chimeric constructs of 5'-and 3'-flanking regions of the pea ferredoxin gene attached to a fi-glucuronidase reporter gene [9]. Both upstream promoter elements and regions of transcribed sequence were shown to be involved in lightregulated gene expression, although there were differences in their relative contributions in greening seedlings and green leaves [13,14]. The 5'-untranslated region of the pea ferredoxin gene was able to confer light responsiveness on reporter genes in transgenic tobacco [7], although no evidence for light-regulatory elements in a similar region of the Arabidopsis gene was found when expressed in transgenic Arabidopsis plants [4].…”

Section: Introductionmentioning

confidence: 99%

Developmental, circadian and light regulation of wheat ferredoxin gene expression

1995

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A genomic clone encoding the precursor of wheat leaf ferredoxin has been isolated and characterised. The uninterrupted PetF gene encodes a polypeptide of 143 amino acid residues, consisting of an N-terminal presequence of 46 amino acid residues and a mature polypeptide of 97 amino acid residues. Southern blot analysis suggests that six copies of the PetF gene are present in the wheat haploid genome. Northern blot analysis has shown that the genes are both developmentally and light regulated in wheat seedlings and provides evidence that a circadian rhythm regulates the steady-state levels of ferredoxin transcripts. The intact wheat gene and several chimeric constructs, containing portions of the 5'-upstream region fused to the beta-glucuronidase reporter gene, have been introduced into tobacco plants, but levels of beta-glucuronidase activity above background were not detected, suggesting that the 5'-upstream region is unable to function as a promoter in tobacco plants.

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Section: Introductionmentioning

confidence: 99%

Developmental, circadian and light regulation of wheat ferredoxin gene expression

1995

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“…In transgenic tobacco seedlings, Fed-i exhibits light ( Oxford University Press responses similar to those of the endogenous tobacco gene (Gallo-Meagher et al, 1992b). Experiments with chimeric genes indicate that the initial light induction in etiolated seedlings is mediated largely by upstream regulatory elements (Gallo-Meagher et al, 1992a); however, the situation in light-grown leaves is quite different.…”

Section: Introductionmentioning

confidence: 99%

Light regulatory sequences are located within the 5′ portion of the Fed-1 message sequence.

1992

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We have previously shown that element(s) mediating a light‐induced increase in the abundance of Fed‐1 mRNA in the leaves of transgenic tobacco plants are located within the transcribed portion of the gene. As part of an effort to define the mechanism of this effect, we report here that cis‐acting elements capable of mediating a 5‐fold light‐induced increase in the abundance of this mRNA are located within a region comprising the 5′ leader and first third of the Fed‐1 coding sequence. No activity was detected in the 3′ untranslated region of the gene. In a gain‐of‐function assay, the 5′ region was found to be capable of conferring light responsiveness on three different reporter sequences, although experiments with the gusA reporter were complicated by an apparent negative light effect on the stability of this mRNA. Deletion experiments show that at least one essential light regulatory element is located in the 5′ untranslated region of Fed‐1 between nucleotides +19 and +57. Additional Fed‐1 sequences, including a portion of the protein coding region, are required to confer positive responsiveness on the gusA reporter. These additional sequences may include specific light regulatory elements or simply provide an environment in which the leader element can function normally.

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“…Gallo-Meagher et al (1992) have monitored Fed-1 (encoding ferredoxin I) mRNA levels in etiolated transgenic tobacco seedlings containing the intact pea Fed-1 gene to determine if the characteristic light responses of this gene in pea seedlings are also observed in transgenic tobacco. It was found that the red-light response of the Fed-1 transgene was closely comparable to that of endogenous tobacco ferredoxin transcripts, and it was concluded that the pea Fed-1 transgene responds normally to tobacco gene-regulatory factors.…”

Section: Discussionmentioning

confidence: 99%

Response of a nitrite-reductase 3.1-kilobase upstream regulatory sequence from spinach to nitrate and light in transgenic tobacco

Neininger

Bichler²,

Schneiderbauer³

et al. 1993

Planta

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In the present study the question was addressed of whether the nitrite-reductase (NIR-)promoter from spinach (Spinacia oleracea L.), fused to a reporter gene (bacterial β-glucuronidase, GUS) and introduced into tobacco (Nicotiana tabacum L.) responds to nitrate and light in accordance with spinach (donor) or in accordance with tobacco (host). The data obtained at the GUS enzyme level as well as at the transcript level allow an unambiguous answer to this question: GUS gene expression under the control of the NIR-promoter from spinach responds to nitrate and light in accordance with the host (tobacco). Expression of the promoter-less GUS gene was not induced by any treatment.

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The pea ferredoxin I gene exhibits different light responses in pea and tobacco.

Cited by 13 publications

References 15 publications

Developmental, circadian and light regulation of wheat ferredoxin gene expression

Developmental, circadian and light regulation of wheat ferredoxin gene expression

Light regulatory sequences are located within the 5′ portion of the Fed-1 message sequence.

Response of a nitrite-reductase 3.1-kilobase upstream regulatory sequence from spinach to nitrate and light in transgenic tobacco

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