Developmental, circadian and light regulation of wheat ferredoxin gene expression

Bringloe, David; Dyer, Tristan A.; Gray, John C.

doi:10.1007/bf00020184

Cited by 21 publications

(7 citation statements)

References 65 publications

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“…Black and white boxes, dark and light periods, respectively levels increased before the subjective day whereas under light/dark cycles the increase began only after transfer to light. This dierence in the kinetics of mRNA accumulation is in agreement with the ®ndings obtained for the control of wheat Fd expression by a circadian clock (Bringloe et al 1995). These results clearly demonstrate the eect of the circadian oscillator since the ®ve mRNAs returned to their basal level even after 44 h in continuous light.…”

Section: Resultssupporting

confidence: 90%

“…However, little is known about the regulation of the genes encoding the enzymes related to this system. Ferredoxin gene expression has been studied in Arabidopsis (Vorst et al 1990), pea (Dobres et al 1987) and wheat (Bringloe et al 1995) and FNR expression in soybean (Neuhaus et al 1997) and spinach (OelmuÈ ller et al 1993). It has been shown that these genes are regulated by light.…”

Section: Introductionmentioning

confidence: 99%

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The complex regulation of ferredoxin/thioredoxin-related genes by light and the circadian clock

Lemaire

Stein

Issakidis‐Bourguet

et al. 1999

Planta

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The biochemical properties of the ferredoxin/thioredoxin transduction pathway regulating the activity of key carbon-fixation enzymes through post-translational modifications are well characterized but little is known about the regulation of the different genes. In the present study, we investigated in Chlamydomonas reinhardtii the regulation of the expression of ferredoxin, thioredoxin m, ferredoxin-NADP reductase, phosphoribulokinase, as well as that of cytosolic thioredoxin h, the function of which is still largely unknown. The effects of light, the circadian clock and active cell division were investigated by northern blotting. The five genes were found to be regulated by light and the circadian clock but with different kinetics and amplitudes. This leads for the first time to the proposal that an extra-chloroplastic thioredoxin is possibly implicated in light and/or circadian-related processes. An interplay between several light-transduction pathways in controlling the expression of the genes is suggested by the expression studies and the theoretical analysis of the promoters.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The complex regulation of ferredoxin/thioredoxin-related genes by light and the circadian clock

Lemaire

Stein

Issakidis‐Bourguet

et al. 1999

Planta

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“…Ferredoxin expression peaked during the light cycle (Figure 4A); its expression pattern was opposite to that of flavodoxin which peaked during the dark cycle (Figure 4B). Regulation of ferredoxin by diel cycles has been documented in higher plants such as pea (Dobres et al, 1987) and wheat (Bringloe et al, 1995), and the green alga, Chlamydomonas reinhardtii (Lemaire et al, 1999). There have not been previous studies in eukaryotic phototrophs assaying flavodoxin in response to diel variations.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Putative Iron Responsive Genes as Species-Specific Indicators of Iron Stress in Thalassiosiroid Diatoms

Whitney¹,

Lins²,

Hughes³

et al. 2011

Front. Microbio.

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Iron (Fe) availability restricts diatom growth and primary production in large areas of the oceans. It is a challenge to assess the bulk Fe nutritional health of natural diatom populations, since species can differ in their physiological and molecular responses to Fe limitation. We assayed expression of selected genes in diatoms from the Thalassiosira genus to assess their potential utility as species-specific molecular markers to indicate Fe status in natural diatom assemblages. In this study, we compared the expression of the photosynthetic genes encoding ferredoxin (a Fe-requiring protein) and flavodoxin (a Fe-free protein) in culture experiments with Fe replete and Fe stressed Thalassiosira pseudonana (CCMP 1335) isolated from coastal waters and Thalassiosira weissflogii (CCMP 1010) isolated from the open ocean. In T. pseudonana, expression of flavodoxin and ferredoxin genes were not sensitive to Fe status but were found to display diel periodicities. In T. weissflogii, expression of flavodoxin was highly responsive to iron levels and was only detectable when cultures were Fe limited. Flavodoxin genes have been duplicated in most diatoms with available genome data and we show that T. pseudonana has lost its copy related to the Fe-responsive copy in T. weissflogii. We also examined the expression of genes for a putative high affinity, copper (Cu)-dependent Fe uptake system in T. pseudonana. Our results indicate that genes encoding putative Cu transporters, a multi-Cu oxidase, and a Fe reductase are not linked to Fe status. The expression of a second putative Fe reductase increased in Fe limited cultures, but this gene was also highly expressed in Fe replete cultures, indicating it may not be a useful marker in the field. Our findings highlight that Fe metabolism may differ among diatoms even within a genus and show a need to validate responses in different species as part of the development pipeline for genetic markers of Fe status in field populations.

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“…Several plant genes have circadian oscillations in transcription, including those for cab (Millar and Kay, 1991;Kellmann et al, 1993), Fd (Bringloe et al, 1995), catalase (Zhong et al, 1994), and rca (Martino-Catt and Ort, 1992). Frequently, however, there are not corresponding oscillations in enzyme activity because of large, stable protein pools.…”

Section: Discussionmentioning

confidence: 99%

Chilling Delays Circadian Pattern of Sucrose Phosphate Synthase and Nitrate Reductase Activity in Tomato1

Jones¹,

Tucker²,

Ort

1998

Plant Physiology

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Overnight low-temperature exposure inhibits photosynthesis in chilling-sensitive species such as tomato (Lycopersicon esculentum) and cucumber by as much as 60%. In an earlier study we showed that one intriguing effect of low temperature on chilling-sensitive plants is to stall the endogenous rhythm controlling transcription of certain nuclear-encoded genes, causing the synthesis of the corresponding transcripts and proteins to be mistimed when the plant is rewarmed. Here we show that the circadian rhythm controlling the activity of sucrose phosphate synthase (SPS) and nitrate reductase (NR), key control points of carbon and nitrogen metabolism in plant cells, is delayed in tomato by chilling treatments. Using specific protein kinase and phosphatase inhibitors, we further demonstrate that the chilling-induced delay in the circadian control of SPS and NR activity is associated with the activity of critical protein phosphatases. The sensitivity of the pattern of SPS activity to specific inhibitors of transcription and translation indicates that there is a chilling-induced delay in SPS phosphorylation status that is caused by an effect of low temperature on the expression of a gene coding for a phosphoprotein phosphatase, perhaps the SPS phosphatase. In contrast, the chilling-induced delay in NR activity does not appear to arise from effects on NR phosphorylation status, but rather from direct effects on NR expression. It is likely that the mistiming in the regulation of SPS and NR, and perhaps other key metabolic enzymes under circadian regulation, underlies the chilling sensitivity of photosynthesis in these plant species.Most warm-climate plant species are sensitive to brief exposures to low, nonfreezing temperatures. Lowtemperature exposure in combination with high irradiance causes rapid, often very severe inhibition of photosynthesis in a broad range of plants, including maize (Baker et al., 1983), cucumber (Peeler and Naylor, 1988), and tomato (Lycopersicon esculentum) (Martin and Ort, 1985). Several elements that contribute to this inhibition have been identified and all may ultimately arise from the photosynthetic production of oxygen radicals (Wise, 1995). An inhibition of electron-transport capacity originating from damage to the reducing side of PSII is well documented (e.g. Powles et al., 1983;Kee et al., 1986; Percival et al., 1987) and, for moderately sensitive species such as maize, may be the major cause of impaired whole-plant photosynthesis after chilling. However, in the most severely chilling-sensitive species, such as domestic tomato, impaired reductive activation of the stromal bisphosphatases appears to be the dominating factor limiting carbon assimilation after chilling in the light (Sassenrath et al., 1990).Low temperature at night can also cause severe reductions in CO 2 fixation that persist on the day after the chill, even after optimal growth temperatures have been restored. Like the inhibition caused by chilling in the light, it is clear that the primary loss of activity is caused b...

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Developmental, circadian and light regulation of wheat ferredoxin gene expression

Cited by 21 publications

References 65 publications

The complex regulation of ferredoxin/thioredoxin-related genes by light and the circadian clock

The complex regulation of ferredoxin/thioredoxin-related genes by light and the circadian clock

Characterization of Putative Iron Responsive Genes as Species-Specific Indicators of Iron Stress in Thalassiosiroid Diatoms

Chilling Delays Circadian Pattern of Sucrose Phosphate Synthase and Nitrate Reductase Activity in Tomato1

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