The pattern of pleiomorphism in stressed Salmonella Virchow populations is nutrient and growth phase dependent

McMahon, M.A.S.; McDowell, D.A.; Blair, I.S.

doi:10.1111/j.1472-765x.2007.02187.x

Cited by 16 publications

(10 citation statements)

References 26 publications

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“…Like S. Typhimurium DT104 in this study, other bacteria such as L. monocytogenes and S. Virchow have also shown a decrease in cell size (3, 40). DT104 transitioning back to the rod shape from the coccoid shape suggests that the cell formation is dependent upon the available nutrients.…”

Section: Discussionsupporting

confidence: 50%

The Effect of Previous Life Cycle Phase on the Growth Kinetics, Morphology and Antibiotic Resistance ofSalmonellaTyphimurium DT104 in Brain Heart Infusion and Ground Chicken Extract

Hawkins¹,

Uknalis

Oscar

et al. 2018

Preprint

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17Growth models are predominately used in the food industry to estimate the potential growth of 18 select microorganisms under environmental conditions. The growth kinetics, cellular 19 morphology and antibiotic resistance were studied throughout the life cycle of Salmonella 20Typhimurium. The effect of the previous life cycle phase ( (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

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Section: Discussionsupporting

confidence: 50%

The Effect of Previous Life Cycle Phase on the Growth Kinetics, Morphology and Antibiotic Resistance ofSalmonellaTyphimurium DT104 in Brain Heart Infusion and Ground Chicken Extract

Hawkins¹,

Uknalis

Oscar

et al. 2018

Preprint

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“…As a consequence, the adaptation response may result in a modified expression of physiological and phenothypical characteristics. For example, authors refer that stress factors such as salinity, nutrient depletion, temperature, pH and atmosphere affect bacterial cell shape, suggesting that morphological changes are correlated to adaptive mechanisms that allow bacteria to prolong survival under adverse conditions (Mattick et al 2000(Mattick et al , 2003aEveris and Betts 2001;Alonso et al 2002;Shi and Xia 2003;Alterman et al 2004;Jydegaard-Axelsen et al 2005;Piuri et al 2005;McMahon et al 2007;Pianetti et al 2009). Furthermore, several studies report that pathogenic bacteria may modulate their virulence properties in response to stress conditions, therefore influencing the outcome of the infectious process in the host.…”

Section: Introductionmentioning

confidence: 99%

Changes in adhesion ability of Aeromonas hydrophila during long exposure to salt stress conditions

et al. 2012

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Aims Stressful environmental conditions influence both bacterial growth and expression of virulence factors. In the present study, we evaluated the influence of NaCl on Aeromonas hydrophila adhesiveness at two temperatures. This agent is often involved in clinical cases; however, its pathogenic potential is still not fully understood. Methods and Results Bacteria were grown in presence of 1·7%, 3·4%, 6·0% NaCl over a 188 day period and then reinoculated in fresh Nutrient Broth with incubation at 4 and 24°C. Bacterial adhesiveness was tested on Hep‐2 cells, and specimens were processed for light, scanning and transmission electron microscopy. Adhesive capacity decreased over time with an increase in reduction percentages depending on NaCl concentrations. At 1·7% NaCl, the reduction was apparently temporary and adhesiveness rapidly recovered in revitalized bacteria, while 3·4%, 6·0% NaCl seemed to be detrimental. Normal, elongated and filamentous bacteria retained adhesiveness capability, although with reduced expression, while in spherical cells, this property seemed to be lost or dramatically reduced. Conclusions Our study shows that high osmolarity plays a significant role in adhesion inhibition, therefore having possible implications in the pathogenesis of the infections by Aer. hydrophila. Significance and Impact of the Study This study intends to give a contribution to a better understanding of the pathogenic role of this bacterium whose pathogenicity is still under debate.

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“…Osmotic stress significantly influences the cell morphology of bacteria [22]. To investigate the effect of Ds-26-16 and EP-5 on cell morphology, scanning electron microscope (SEM) was employed to examine the E .…”

Section: Resultsmentioning

confidence: 99%

“…Such cells have intact membranes and could revert to normal morphology upon removal of stress. Therefore, cell filamentation could be considered as a means of bacteria adaptation in unfavorable conditions [22]. This has been reported in a number of organisms including Gluconacetobacter diazotrophicus PAL5 [27], Salmonella [22], and also E .…”

Section: Discussionmentioning

confidence: 99%

Directed Evolution of Dunaliella salina Ds-26-16 and Salt-Tolerant Response in Escherichia coli

Guo

Dong

Xiao

et al. 2016

IJMS

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Identification and evolution of salt tolerant genes are crucial steps in developing salt tolerant crops or microorganisms using biotechnology. Ds-26-16, a salt tolerant gene that was isolated from Dunaliella salina, encodes a transcription factor that can confer salt tolerance to a number of organisms including Escherichia coli (E. coli), Haematococcus pluvialis and tobacco. To further improve its salt tolerance, a random mutagenesis library was constructed using deoxyinosine triphosphate-mediated error-prone PCR technology, and then screened using an E. coli expression system that is based on its broad-spectrum salt tolerance. Seven variants with enhanced salt tolerance were obtained. Variant EP-5 that contained mutation S32P showed the most improvement with the E. coli transformant enduring salt concentrations up to 1.54 M, in comparison with 1.03 M for the wild type gene. Besides, Ds-26-16 and EP-5 also conferred E. coli transformant tolerance to freezing, cold, heat, Cu2+ and alkaline. Homology modeling revealed that mutation S32P in EP-5 caused the conformational change of N- and C-terminal α-helixes. Expression of Ds-26-16 and EP-5 maintained normal cellular morphology, increased the intracellular antioxidant enzymatic activity, reduced malondialdehyde content, and stimulated Nitric Oxide synthesis, thus enhancing salt tolerance to E. coli transformants.

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The pattern of pleiomorphism in stressed Salmonella Virchow populations is nutrient and growth phase dependent

Abstract: The application of sublethal concentrations of salt may stimulate S. Virchow morphotype diversity, improving survival and rates of poststress recovery.

Cited by 16 publications

References 26 publications

The Effect of Previous Life Cycle Phase on the Growth Kinetics, Morphology and Antibiotic Resistance ofSalmonellaTyphimurium DT104 in Brain Heart Infusion and Ground Chicken Extract

The Effect of Previous Life Cycle Phase on the Growth Kinetics, Morphology and Antibiotic Resistance ofSalmonellaTyphimurium DT104 in Brain Heart Infusion and Ground Chicken Extract

Changes in adhesion ability of Aeromonas hydrophila during long exposure to salt stress conditions

Directed Evolution of Dunaliella salina Ds-26-16 and Salt-Tolerant Response in Escherichia coli

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