The pattern of gene expression in human CD34 ⁺ stem/progenitor cells

Abstract

We have analyzed the pattern of gene expression in human primary CD34 ؉ stem͞progenitor cells. We identified 42,399 unique serial analysis of gene expression (SAGE) tags among 106,021 SAGE tags collected from 2.5 ؋ 10 6 CD34 ؉ cells purified from bone marrow. Of these unique SAGE tags, 21,546 matched known expressed sequences, including 3,687 known genes, and 20,854 were novel without a match. The SAGE tags that matched known sequences tended to be at higher levels, whereas the novel SAGE tags tended to be at lower levels. By using the generation of longer sequences from SAGE tags for gene identification (GLGI) method, we identified the correct gene for 385 of 440 high-copy SAGE tags that matched multiple genes and we generated 198 novel 3 expressed sequence tags from 138 high-copy novel SAGE tags. We observed that many different SAGE tags were derived from the same genes, reflecting the high heterogeneity of the 3 untranslated region in the expressed genes. We compared the quantitative relationship for genes known to be important in hematopoiesis. The qualitative identification and quantitative measure for each known gene, expressed sequence tag, and novel SAGE tag provide a base for studying normal gene expression in hematopoietic stem͞progenitor cells and for studying abnormal gene expression in hematopoietic diseases.

Global transcription analysis of immature avian erythrocytic progenitors: from self-renewal to differentiation

“…The case of Sca-2 gene is interesting. The SCA-2 protein belongs to the Ly-6 family (low molecular weight White bars correspond to the results obtained for SR SAGE library, black bars correspond to the results obtained for Di SAGE library, and gray bars correspond to the results obtained for the analysis of the available SAGE data generated from CD34 þ human stem cells (Zhou et al, 2001). Note than one gene can belong to more than one category, thereby resulting in an overall level of more than 100%.…”

“…(c) Representation of the percentage of genes in each category of GO 'Molecular Function' classification at level 2. White bars correspond to the results obtained for SR SAGE library, black bars correspond to the results obtained for Di SAGE library and gray bars correspond to the results obtained for the analysis of the available SAGE data generated from CD34 þ human stem cells (Zhou et al, 2001). Note than one gene can belong to more than one category, thereby resulting in an overall level of more than 100% SAGE on chicken erythrocytic progenitors F Damiola et al glycophosphatidylinositol-linked proteins) and it is expressed on hematopoietic and lymphoid cells (Antica et al, 1997).…”

“…Of interest is the downregulation of the Btg-1 gene, a gene previously shown to be modulated by glucocorticoids, erythropoietin and stem cell factor in erythroid progenitors (Kolbus et al, 2003). One should also note the downregulation of the Hmg-17 gene, a gene previously shown by SAGE to be very highly expressed in human CD34 þ cells (Zhou et al, 2001).…”

“…In addition, we compared the functions involved in our cells with those obtained from CD34 þ human stem cells (Zhou et al, 2001). Differences appear in the comparison of several GO categories between the two cell types (avian T2EC and human CD34 þ ).…”

Multiplacenta derived stem cell/cytokine treatment increases survival time in a mouse model with radiation-induced bone marrow damage

“…The placenta contains a higher proportion of more primitive hematopoietic cells than the bone marrow (Smogorzewska et al 1997). Placenta-derived hematopoietic stem cells have a greater proliferative response to cytokines than the corresponding cells in the bone marrow or peripheral blood (Smogorzewska et al 1997;Zhou et al 2001). On CD34 ?…”