Multiplacenta derived stem cell/cytokine treatment increases survival time in a mouse model with radiation-induced bone marrow damage

Abstract

Nuclear Warfare and nuclear leakage can result in a large number of patients with radiationinduced bone marrow damage. Based on the fact that hematopoietic stem cells and hematopoietic growth factors are characterized as a novel strategy for therapy, the aim of this study was to explore a safe and routine stem cell/cytokine therapeutic strategy. Allogeneic multiplacenta derived hematopoietic and mesenchymal stem cells/cytokines were intraperitoneally injected into a moderate dose of total body irradiation-induced mouse bone marrow damage model a single time. Then, the mouse posttransplantation survival time, peripheral blood hemoglobin count, bone marrow architecture, and donor cell engraftment were assessed. Each mouse that received placenta-derived stem cells exhibited positive donor hematopoietic and mesenchymal stem cell engraftment both in the bone marrow and peripheral blood after transplantation. The peripheral blood hemoglobin count and survival time were greater in the group with the combined treatment of multiplacenta-derived stem cells and cytokines, compared with model-only controls (both P \ 0.001). The blood smear mesenchymal/hematopoietic stem cell count was significantly higher in the combined treatment group than in the mice treated only with placenta-derived cells (28.08 ± 5.824 vs. 20.40 ± 5.989, P \ 0.001; 7.74 ± 2.153 vs. 4.23 ± 1.608, P \ 0.001, respectively). However, there was no marked change on the bone marrow pathology of any of the experimental mice after the transplantation. These results indicate that for radiation-induced bone marrow damage treatment,

Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats

“…Induction of an acute radiation enteritis model Rats in the 3 experimental groups were subjected to local radiation in the abdominal region at a sublethal dose of 10 Gy (total dose) as we previously described [16][17] . Then, the rats were ready for treatment.…”

“…First, we locked the rats in a device similar to a large conical centrifuge tube to keep the body immobile, and then, an infant scalp venous needle was used for the injection of 1 mL of cells via the tail vein. The model-only control rats received the same volume of Nutrient Mixture F-12 Media via intraperitoneal injection as a vehicle [16][17][18] .…”

“…Then, UCMSCs from the filtrate were repeatedly reproduced and isolated by adherent culture and density gradient centrifugation procedures (Ficoll-Paque Premium, 1.073 g/mL). Finally, passage 2 UCMSCs were identified and marked with Hoechst 33258 [16][17][18] .…”

“…Each sample was smeared on a slide and observed with fluorescence microscopy within a few hours. The image of blue fluorescent protein-positive cells per visual field was acquired at 400Â magnification [16][17] .…”

“…We previously showed that a single intraperitoneal injection of multiplacenta-derived cells can effectively improve the survival time of a mouse model with radiation-induced bone marrow damage 16,17 . In this experimental model, we assessed the survival and transient effects of intraperitoneally injected human multiumbilical cord-derived exogenous MSCs without immunosuppressant intervention.…”