The pathogenic role of pulmonary intravascular macrophages in acute African swine fever

Carrasco, L.; Lara, F. Chacón-M. de; Gómez-Villamandos, J.C.; Bautista, M.J.; Villeda, C. J.; Wilkinson, Philip; Sierra, M. A.

doi:10.1016/s0034-5288(96)90062-4

Cited by 31 publications

(28 citation statements)

References 27 publications

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“…The thickening of alveolar septa was mainly due to an increase in the number of macrophages that, because of their location in the lumen of septal capillaries, may correspond to pulmonary intra‐vascular macrophages. The increase in the number and the activation of pulmonary intra‐vascular macrophages have been described in other viral ( Carrasco et al, 1996) and bacterial ( Bertram, 1986) diseases of the pig, and have been related to an increase in vascular permeability, with the subsequent oedema and microthrombosis in the septal capillaries. Similar changes have already been described in HC ( Van Der Molen and Van Oirschot, 1981; Trautwein, 1988), and suggest that pulmonary intra‐vascular macrophages might play an important role in the pulmonary changes observed in HC, and which are mainly related to the presence of pulmonary oedema and microthrombosis in septal capillaries and to the maintenance of viremia, a role that has also been suggested in the porcine reproductive respiratory syndrome ( Rossow et al, 1995).…”

Section: Discussionmentioning

confidence: 92%

Characterization of Lesions Caused by a South American Virulent Isolate (‘Quillota’) of the Hog Cholera Virus

Quezada

Cayo

Carrasco

et al. 2000

Journal of Veterinary Medicine, Series B

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In this study, macroscopic and histopathological lesions produced by a virulent South American isolate ('Quillota') of hog cholera virus were studied. The virus was inoculated in doses of 10(5)TCID50 in each of 35 pigs of 20 kg live weight. The animals were slaughtered from 4 to 18 days post-inoculation. The presence of virus antigens in lymphatic tissue was confirmed by both direct immunofluorescence and Avidin-Biotin-Peroxidase techniques in formalin-embedded tissue samples. Histological sections were stained with haematoxylin-eosin and Mallory's phosphotungstic acid haematoxylin methods. The 'Quillota' isolate used in this study caused a disease characterized by vascular lesions (splenic infarcts, haemorrhages in the lymph nodes and the urinary system and disseminated microthrombosis), and necrosis of lymphocytes, particularly in the B-areas of the lymphoid organs, lesions that are characteristic of the acute form of the disease. Other lesions observed were a non-purulent meningoencephalitis, the necrosis of the epithelial cells of tonsils, the presence of fibrin nets in the red pulp and a marked thickening of the alveolar septa.

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Section: Discussionmentioning

confidence: 92%

Characterization of Lesions Caused by a South American Virulent Isolate (‘Quillota’) of the Hog Cholera Virus

Quezada

Cayo

Carrasco

et al. 2000

Journal of Veterinary Medicine, Series B

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“…Atembeschwerden mit blutigem Ausfluss aus den Nasenlöchern (Carrasco et al, 1996, blutige Diarrhoe, evtl. Konstipation, Vomitus und Konjunktivitis auftreten (Howey et al, 2013).…”

Section: Klinik Und Pathologieunclassified

3.3 Afrikanische Schweinepest

Mayr¹

2007

Medizinische Mikrobiologie, Infektions- Und Seuchenlehre

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“…Components of MPS have been traditionally identified as the main target cells of CSFV, both in peripheral blood (Summerfield, Knotig, & McCullough, ) and lymphoid organs (Gómez‐Villamandos et al., ; Sánchez‐Cordón et al., ). PIMs, resident population of macrophages in the pulmonary capillaries of certain animal species (Schneberger, Aharonson‐Raz, & Singh, ), have been identified as main responsible of the inflammatory changes appeared in lung during the course of different viral diseases such as African swine fever (Carrasco et al., ), porcine reproductive respiratory syndrome (Gómez‐Laguna et al., ), African horse sickness (Carrasco et al., ) or bovine viral diarrhoea (Risalde et al., ). Previous studies had suggested the potential role of PIMs in the pathogenetic mechanisms of pulmonary lesions during acute forms of CSF through the modulation of local inflammatory and immune responses by releasing different chemical mediators (Carrasco et al., ).…”

Section: Introductionmentioning

confidence: 99%

Pulmonary intravascular macrophages regulate the pathogenetic mechanisms of pulmonary lesions during acute courses of classical swine fever

Núñez

Sánchez-Cordón

Pedrera

et al. 2018

Transbound Emerg Dis

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Classical swine fever (CSF) is a highly contagious and often fatal viral disease of domestic pigs and wild boar. Pulmonary oedema and haemorrhages in lung parenchyma are common lesions in the acute forms of CSF that may compromise pig survival and whose pathogenetic mechanisms remain unclear. The appearance of pulmonary lesions in pigs infected with Alfort/187 strain of classical swine fever virus (CSFV) euthanized between 2 and 17 days postinfection (dpi) and the role played by cytokines secreted by different pulmonary macrophage populations in the evolution of lesions was evaluated in this study. Microscopic changes of alveolar septal thickening along with oedema and haemorrhages became more severe at middle-late stages of the experiment. A significant increase in the number of pulmonary macrophages, mainly pulmonary intravascular macrophages (PIMs), was observed coinciding with the onset of alveolar septal thickening from Day 4 pi. PIMs were the main target of CSFV from initial stages of infection while the presence of infected pulmonary alveolar macrophages (PAMs) was scarce and late. Initial infection of PIMs induced phagocytic and biosynthetic activation with subsequent release of chemotactic cytokines. TNFα and, to a lesser extent, IL-1α secreted by PIMs were the major cytokines involved, while IL-6 played only a minor role. On the contrary, results suggested only a secondary role of PAMs as source of cytokines. The presence of vascular changes from Day 9 pi coincided with the highest levels of infected PIMs and the highest number of pro-inflammatory cytokines secreting PIMs. Activation and phagocytosis of platelets were observed in the lungs of infected pigs from early stages, also coinciding with the expression of cytokines with a proven procoagulant activity. The existence of intravascular coagulation phenomena in lung was ruled out.

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The pathogenic role of pulmonary intravascular macrophages in acute African swine fever

Cited by 31 publications

References 27 publications

Characterization of Lesions Caused by a South American Virulent Isolate (‘Quillota’) of the Hog Cholera Virus

Characterization of Lesions Caused by a South American Virulent Isolate (‘Quillota’) of the Hog Cholera Virus

3.3 Afrikanische Schweinepest

Pulmonary intravascular macrophages regulate the pathogenetic mechanisms of pulmonary lesions during acute courses of classical swine fever

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