The Participation of Mast Cells in Adaptation of the Stomach of Mongolian Gerbils to the Gravitational Factor

Atyakshin, D. A.; Nikityuk, D. B.; Klochkova, S. V.; Alexeeva, N. T.; Burtseva, A. S.

doi:10.18499/2225-7357-2018-7-1-14-26

Cited by 3 publications

(4 citation statements)

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“…For some tissues and animal species, an immunohistochemical reaction on c-kit was demonstrated to reveal mast cells better than toluidine blue [122], and not less effectively than tryptase immunohistochemistry [123]. However, other studies revealed that this marker is not sufficiently specific, and REVIEWS using antibodies to c-kit it is possible to image only a part of mast cells, which are immunopositive to tryptase or chymase [124,125], and vice versa, only a part of c-kit-immunoreactive cells were also immunopositive to tryptase or chymase [125]. This is due to the fact that c-kit receptor, in addition to mastocytes, is detected in stem and germinal cells, epithelial cells of sweat glands, ducts of the mammary gland, renal and testicular tubules, interstitial cells of Cajal, melanocytes, and basal cells of the epidermis, some neurons and gliocytes of the brain [126][127][128].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Modern Imaging Technologies of Mast Cells for Biology and Medicine (Review)

Grigor’ev¹,

Коржевский²

2021

Sovrem Tehnol Med

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Mast cells play an important role in the body defense against allergens, pathogens, and parasites by participating in inflammation development. However, there is evidence for their contributing to the pathogenesis of a number of atopic, autoimmune, as well as cardiovascular, oncologic, neurologic, and other diseases (allergy, asthma, eczema, rhinitis, anaphylaxis, mastocytosis, multiple sclerosis, rheumatoid arthritis, inflammatory gastrointestinal and pulmonary diseases, migraine, etc.). The diagnosis of many diseases and the study of mast cell functions in health and disease require their identification; so, the knowledge on adequate imaging techniques for mast cells in humans and different species of animals is of particular importance.The present review summarizes the data on major methods of mast cell imaging: enzyme histochemistry, immunohistochemistry, as well as histochemistry using histological stains. The main histological stains bind to heparin and other acidic mucopolysaccharides contained in mast cells and stain them metachromatically. Among these are toluidine blue, methylene blue (including that contained in May-Grünwald-Giemsa stain), thionin, pinacyanol, and others. Safranin and fluorescent dyes: berberine and avidin -also bind to heparin. Longer staining with histological dyes or alcian blue staining is needed to label mucosal and immature mast cells.Advanced techniques -enzyme histochemistry and especially immunohistochemistry -enable to detect mast cells high-selectively using a reaction to tryptases and chymases (specific proteases of these cells). In the immunohistochemical study of tryptases and chymases, species-specific differences in the distribution of the proteases in mast cells of humans and animals should be taken into account for their adequate detection. The immunohistochemical reaction to immunoglobulin E receptor (FcεRI) and c-kit receptor is not specific to mast cells, although the latter is important to demonstrate their proliferation in normal and malignant growth.Correct fixation of biological material is also discussed in the review as it is of great significance for histochemical and immunohistochemical mast cell detection.Fluorescent methods of immunohistochemistry and a multimarker analysis in combination with confocal microscopy are reported to be new technological approaches currently used to study various mast cell populations.

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Section: Immunohistochemistrymentioning

confidence: 99%

Modern Imaging Technologies of Mast Cells for Biology and Medicine (Review)

Grigor’ev¹,

Коржевский²

2021

Sovrem Tehnol Med

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“…This allows admitting the presence of either direct effects of cosmic radiation on the crosslinking of amino acids in collagen molecules, or the phenomena of disorganization of fibrous structures resulted from the developing trophic disorders. In addition, it is impossible to exclude the influence of matrix metalloproteinases, which can be actively functioning under conditions of gravitational unloading, including the influence of mast cell proteases [23][24][25]. In particular, the bioeffects of cosmic radiation were demonstrated in an experiment on a cultured human fibroblasts exposed for 14 days aboard the ISS [26].…”

Section: Discussionmentioning

confidence: 99%

“…A number of experimental studies allow assuming that the state of the intercellular substance of the connective tissue of various gastrointestinal organs after a space flight or under ground-based simulated physiological effects of weightlessness is closely related to the features of mast cell population [3,4,35]. Notably, the active secretion of proteases, tryptase and chymase, whicah are capable of activating matrix metalloproteinases and, as a result, accelerating the degradation of collagen fibers, seems to be especially important [18,21,[23][24][25]36]. The ability of mast cells to migrate in all layers of the walls of hollow organs and to actively secrete various factors determines their potential active participation in the gastric and intestinal stroma remodeling during space flight.…”

Section: Discussionmentioning

confidence: 99%

“…The structures of the connective tissue of internal organs, acting as a soft skeleton, respond to changes in gravity during space flight. As demonstrated earlier in the experiment involving Mongolian gerbils after a 12-day flight on the Foton-M3 spacecraft, specific changes reflecting the result of gravitational unloading developed in the interstitium of the digestive organs [1][2][3][4][5]. The launch of the Russian Bion-M 1 biosatellite provided new opportunities to identify structural and functional effects in the body of mammals after a long stay in the conditions of weightlessness, including the state of the connective tissue of the digestive system.…”

Section: Introductionmentioning

confidence: 99%

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Gravisensitivity Of Stromal Collagen Fibers In The Gastrointestinal Hollow Organs Of Mice After A 30-Day Space Flight

Шишкина¹,

Kostin²,

Alexeeva³

et al. 2023

Preprint

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The digestive organs are highly sensitive to the influence of orbital flight factors and can limit the professional activities of crew members aboard the International Space Station. Connective tissue as a system-forming matrix of the integrative-buffer metabolic environment is of particular relevance in space biomedicine, ensuring the functioning of internal organs under an altered gravitational stimulus. However, the adaptive mechanisms of the fibrous extracellular matrix of the gastric and intestinal connective tissue have not been fully investigated under prolonged microgravity weightlessness. Using histochemical techniques, the authors experimentally studied the state of collagen fibers in the specific tissue microenvironment of the gastric and intestinal membranes in C57BL/6N mice after a 30-day space flight, subsequent 7-day ground readaptation, and in animals of the relevant control groups. The 30-day stay of laboratory animals aboard the Bion-M 1 biosatellite resulted in a reduction in the fibrous extracellular matrix of connective tissue in the studied digestive organs, excepting the gastric lamina propria. An increased fibrillogenesis was revealed in the gastrointestinal mucous membranes of animals in 7 days after the biosatellite landing, if compared with the parameters of animals of the space flight group. During the experiment with ground simulated orbital flight conditions, changes in collagen fibers were not significant compared to the vivarium control group. Thus, the results obtained evidence gravisensitivity of the fibrous extracellular matrix of the intraorgan connective tissue. This fact also highlights the necessity to further improve gastrointestinal tract-related preventive measures for astronauts during orbital flight.

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