The paracrine effects of adipose-derived stem cells on neovascularization and biocompatibility of a macroencapsulation device

Wang, Kai; Yu, Lang; Jiang, Li-Yang; Wang, Haibin; Wang, Changyong; Luo, Ying

doi:10.1016/j.actbio.2014.12.025

Cited by 40 publications

(35 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When loaded with cells, the paracrine factors generated by these cells would be able to diffuse out of the device and promote neovascularization of the device. The effects were reported previously by our group [17] and how to take advantage of the encapsulated cells to modulate the tissue responses warrants exploration in the future. Even though the nanofibrous materials are biocompatible, the total surface area or vessel numbers of membrane-based devices may only support certain amounts of cells.…”

Section: Allotransplantation Of Cells Via Pu-nano-based Macroencapsulmentioning

confidence: 66%

“…Immunoisolation devices are preferred to prevent the potential risks or immune recognition of the transplanted cells [1,2,17]. It is possible to take advantage of encapsulation devices as well as the paracrine products of the implanted cells to develop treatments for autoimmune diseases [40], acute liver failure [41] and diabetes [5].…”

Section: Allotransplantation Of Cells Via Pu-nano-based Macroencapsulmentioning

confidence: 99%

“…The macroencapsulation devices were fabricated as previously reported [17]. Briefly, two inner layers of electrospun membranes were sandwiched between two outer layers of polyethylene terephthalate (PET) mesh (SAATItech™, Satti, Italy).…”

Section: Fabrication Of Macroencapsulation Devicementioning

confidence: 99%

“…Rat adipose derived mesenchymal stem cells (AD-MSCs) were obtained from the subcutaneous adipose tissue of male SpragueeDawley rats (Vital River Laboratory, China) weighing 120 g according to a reported protocol [17]. AD-MSCs were cultured and passaged in the MEM Alpha supplemented with 10% (v/v) fetal bovine serum (FBS) and 0.4% penicillin/streptomycin (P/S).…”

Section: Cell Preparation and Culturementioning

confidence: 99%

See 3 more Smart Citations

Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane

et al. 2016

Self Cite

View full text Add to dashboard Cite

Section: Allotransplantation Of Cells Via Pu-nano-based Macroencapsulmentioning

confidence: 66%

Section: Allotransplantation Of Cells Via Pu-nano-based Macroencapsulmentioning

confidence: 99%

Section: Fabrication Of Macroencapsulation Devicementioning

confidence: 99%

Section: Cell Preparation and Culturementioning

confidence: 99%

See 2 more Smart Citations

Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane

et al. 2016

Self Cite

View full text Add to dashboard Cite

“…This showed that a 3D environment, such as a fibrous mesh, supports the differentiation of at least a subset of ASCs towards the endothelial cell phenotype when co-cultured with a seeded 2D film. This emphasises the importance of the paracrine function by cells seeded on a 3D mesh or a 2D film (Wang et al, 2015) secreting soluble factors during their early differentiation. These factors influenced the cells seeded on the films underneath (and vice versa) and thereby initiated a response.…”

Section: Discussionmentioning

confidence: 99%

Gene expression in human adipose-derived stem cells: comparison of 2D films, 3D electrospun meshes or co-cultured scaffolds with two-way paracrine effects

Hess

Stark

Mohn

et al. 2017

eCM

View full text Add to dashboard Cite

Finding the appropriate cues to trigger the desired differentiation is a challenge in tissue engineering when stem cells are involved. In this regard, three-dimensional environments are often compared to cells' twodimensional culture behaviour (plastic culture dish). Here, we compared the gene expression pattern of human adipose-derived stem cells (ASC) seeded in a three-dimensional (3D) electrospun mesh and on a two-dimensional (2D) film -both of exactly the same material. Additionally, we conducted experiments with a scaffold floating above a film to investigate two-way paracrine effects (co-system). Electrospun meshes (3D scaffolds) and films (2D), consisting either of pristine poly-lactic-co-glycolic acid (PLGA) or of PLGA-containing dispersed amorphous calcium phosphate nanoparticles (PLGA/aCaP), were seeded with ASCs and cultured either in Dulbecco Minimum Essential Medium (DMEM) or in osteogenic medium. After two weeks, minimum stem cell criteria markers as well as typical markers for osteogenesis, endothelial cell differentiation, adipogenesis and chondrogenesis were analysed by quantitative real-time PCR. Interestingly, mostly osteogenic genes of cells seeded on 3D meshes were upregulated compared to those on 2D films, while stem cell markers seemed to be only slightly affected. Runx2 and osteocalcin showed an especially strong upregulation under all conditions, while most other factors analysed for 2D/3D changes were highly dependent on the material composition, the culture medium and on paracrine signalling effects. The beneficial 3D environment for stem cells found in many studies has therefore not to be attributed to the third dimension alone and should carefully be compared to 2D films fabricated of the same material. Furthermore, paracrine interactions triggering differentiation are not negligible.

show abstract

Embedded Spheroids as Models of the Cancer Microenvironment

2017

View full text Add to dashboard Cite

To more accurately study the complex mechanisms behind cancer invasion, progression, and response to treatment, researchers require models that replicate both the multicellular nature and 3D stromal environment present in an in vivo tumor. Multicellular aggregates (i.e., spheroids) embedded in an extracellular matrix mimic are a prevalent model. Recently, quantitative metrics that fully utilize the capability of spheroids are described along with conventional experiments, such as invasion into a matrix, to provide additional details and insights into the underlying cancer biology. The review begins with a discussion of the salient features of the tumor microenvironment, introduces the early work on non-embedded spheroids as tumor models, and then concentrates on the successes achieved with the study of embedded spheroids. Examples of studies include cell movement, drug response, tumor cellular heterogeneity, stromal effects, and cancer progression. Additionally, new methodologies and those borrowed from other research fields (e.g., vascularization and tissue engineering) are highlighted that expand the capability of spheroids to aid future users in designing their cancer-related experiments. The convergence of spheroid research among the various fields catalyzes new applications and leads to a natural synergy. Finally, the review concludes with a reflection and future perspectives for cancer spheroid research.

show abstract

The paracrine effects of adipose-derived stem cells on neovascularization and biocompatibility of a macroencapsulation device

Cited by 40 publications

References 61 publications

Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane

Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane

Gene expression in human adipose-derived stem cells: comparison of 2D films, 3D electrospun meshes or co-cultured scaffolds with two-way paracrine effects

Embedded Spheroids as Models of the Cancer Microenvironment

Contact Info

Product

Resources

About