The p6.5 gene region of a nuclear polyhedrosis virus of Orgyia pseudotsugata: DNA sequence and transcriptional analysis of four late genes

Molecular analysis of the p48 gene of Choristoneura fumiferana multicapsid nucleopolyhedroviruses CfMNPV and CfDEFNPV.

“…In OpMNPV (D), five ORFs, p87, p48, p12, p40 and p6n5, are shown. The complementary RNA (cRNA, with direction shown by arrow) probe for p6n5 was used in Northern blot analysis (Russell & Rohrmann, 1990) and detected a 3200 bp transcript (shown underneath) originating from ORF p48. In AcMNPV (E), the cRNA probe (direction is shown by arrow) of p80 detected a 3100 bp transcript originating from p48 (Lu & Carstens, 1992 The p48 gene of CfMNPV and CfDEFNPV The p48 gene of CfMNPV and CfDEFNPV ognition site that is not normally present into a non-essential region of the genome.…”

“…The 5h end of ORF p12 has a 20 nucleotide overlap with the 3h end of p48. Homologues of ORF p48, p12 and p40 are present in the same tandem arrangement in the genomes of OpMNPV (Russell & Rohrmann, 1990), AcMNPV (Lu & Carstens, 1992) and CfDEFNPV (see below). The EcoRI-N fragment contains three other ORFs, 1, 2 and 3, which could potentially encode proteins of more than 60 amino acids (Fig.…”

“…The p48 ORF is also present in the genomes of Orgyia pseudotsugata (Op) MNPV (Russell & X. Li and others X. Li and others (Arif et al, 1984). Rohrmann, 1990), AcMNPV (Lu & Carstens, 1992), Bombyx mori (Bm) NPV (GenBank accession no. L33180) and CfDEFNPV.…”

Immunocytochemical characterization of p24, a baculovirus capsid-associated protein

“…In addition to containing the genome and a putative DNA-binding protein of 6.5K to 7.5K (Wilson et al, 1987;Russell & Rohrmann, 1990b;Maeda et al, 1991), PDV and BV nucleocapsids both appear to have at least two capsid-associated proteins. The p39 protein is a major capsid component and has been described from three NPVs (Pearson et al, 1988;Blissard et al, 1989;Thiem & Miller, 1989;Bjornson & Rohrmann, 1992b).…”

Characterization of an early gene coding for a highly basic 8.9K protein from the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus

“…1 b, c). Other highly basic baculovirus proteins have been described, such as the OpMNPV p6.5 and the AcMNPV homologue p6"9 (Wilson et aL, 1987;Wilson, 1988;Russell & Rohrmann, 1990). p6"5 is highly basic as it is rich in arginine residues and contains many serines and threonines in addition.…”