The oxidative stress response of the opportunistic fungal pathogen Candida glabrata

Briones-Martin-del-Campo, Marcela; Orta-Zavalza, Emmanuel; Juárez-Cepeda, Jacqueline; Gutiérrez-Escobedo, Guadalupe; Cañas-Villamar, Israel; Castaño, Irene; Peñas, Alejandro De Las

doi:10.1016/j.riam.2013.09.012

Cited by 37 publications

(28 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, no correlation was observed between the activity of this enzyme and the antifungal susceptibility of these clinical isolates. C. glabrata possesses both enzymatic and glutathione mechanisms to resist to the oxidative stress induced by the host immune defenses [75], and our results reinforce the importance of enzymatic mechanisms to maintain redox homeostasis in clinical C. glabrata strains.…”

Section: Discussionsupporting

confidence: 78%

Relationship between the Antifungal Susceptibility Profile and the Production of Virulence-Related Hydrolytic Enzymes in Brazilian Clinical Strains ofCandida glabrata

Figueiredo-Carvalho

Ramos

Barbedo

et al. 2017

Mediators of Inflammation

View full text Add to dashboard Cite

Candida glabrata is a facultative intracellular opportunistic fungal pathogen in human infections. Several virulence-associated attributes are involved in its pathogenesis, host-pathogen interactions, modulation of host immune defenses, and regulation of antifungal drug resistance. This study evaluated the in vitro antifungal susceptibility profile to five antifungal agents, the production of seven hydrolytic enzymes related to virulence, and the relationship between these phenotypes in 91 clinical strains of C. glabrata. All C. glabrata strains were susceptible to flucytosine. However, some of these strains showed resistance to amphotericin B (9.9%), fluconazole (15.4%), itraconazole (5.5%), or micafungin (15.4%). Overall, C. glabrata strains were good producers of catalase, aspartic protease, esterase, phytase, and hemolysin. However, caseinase and phospholipase in vitro activities were not detected. Statistically significant correlations were identified between micafungin minimum inhibitory concentration (MIC) and esterase production, between fluconazole and micafungin MIC and hemolytic activity, and between amphotericin B MIC and phytase production. These results contribute to clarify some of the C. glabrata mechanisms of pathogenicity. Moreover, the association between some virulence attributes and the regulation of antifungal resistance encourage the development of new therapeutic strategies involving virulence mechanisms as potential targets for effective antifungal drug development for the treatment of C. glabrata infections.

show abstract

Section: Discussionsupporting

confidence: 78%

Relationship between the Antifungal Susceptibility Profile and the Production of Virulence-Related Hydrolytic Enzymes in Brazilian Clinical Strains ofCandida glabrata

Figueiredo-Carvalho

Ramos

Barbedo

et al. 2017

Mediators of Inflammation

View full text Add to dashboard Cite

show abstract

“…2). Phagocytes produce reactive oxygen species through the pathogen-host interaction-mediated pathway [1,4,7]. Oxidative stress can result in DNA damage.…”

Section: Discussionmentioning

confidence: 99%

“…C. glabrata has a well-defined oxidative stress response, which includes both enzymatic and nonenzymatic mechanisms. This stress response is controlled by the well-conserved transcription factors Yap1, Skn7, Msn2 and Msn4 [4]. The response of C. albicans to oxidative stress is mediated by oxidant-specific regulation of translation initiation [21].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Micronuclei, Nuclear Anomalies and the Nuclear/Cytoplasmic Ratio of Exfoliated Cervical Epithelial Cells in Genital Candidiasis

Öz¹,

Gün

Özdamar

2015

Acta Cytologica

View full text Add to dashboard Cite

Objective:Candida is the most common cause of fungal infections. The aim of this study was to fill the gaps in the current knowledge on the frequencies of micronuclei and nuclear anomalies, and the nucleus/cytoplasmic ratio in genital candidiasis. Study Design: A total of 88 Papanicolaou- stained cervical smears, which comprised Candida spp. (n = 44) and control cases with no infectious agent (n = 44), were studied. In each smear, cells with micronuclei and nuclear anomalies were counted in 1,000 epithelial cells and also nuclear and cellular areas were evaluated using image analysis software at a magnification of ×400. Results: The frequencies of micronucleated and binucleated cells and cells with perinuclear halos, and the nucleus/cytoplasmic ratio of epithelial cells were higher in the Candida-infected group compared with the control group (p < 0.05). Conclusions: Genital candidiasis is able to induce changes in the size and shape of epithelial cells. The nuclear/cytoplasmic ratio and the frequency of micronuclei may reflect the DNA damage in the cervical epithelium. Micronucleus scoring could be used to screen the genomic damage profile of epithelial cells in candidiasis.

show abstract

“…Candida spp. also possess several enzymes that function in a protective manner against the respiratory burst, such CATs, SODs, and glutathione peroxidases (Briones-Martin-Del-Campo et al, 2014). …”

Section: Attackmentioning

confidence: 99%

Anti-Immune Strategies of Pathogenic Fungi

Marcos¹,

Oliveira²,

Melo³

et al. 2016

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Pathogenic fungi have developed many strategies to evade the host immune system. Multiple escape mechanisms appear to function together to inhibit attack by the various stages of both the adaptive and the innate immune response. Thus, after entering the host, such pathogens fight to overcome the immune system to allow their survival, colonization and spread to different sites of infection. Consequently, the establishment of a successful infectious process is closely related to the ability of the pathogen to modulate attack by the immune system. Most strategies employed to subvert or exploit the immune system are shared among different species of fungi. In this review, we summarize the main strategies employed for immune evasion by some of the major pathogenic fungi.

show abstract

The oxidative stress response of the opportunistic fungal pathogen Candida glabrata

Cited by 37 publications

References 41 publications

Relationship between the Antifungal Susceptibility Profile and the Production of Virulence-Related Hydrolytic Enzymes in Brazilian Clinical Strains ofCandida glabrata

Relationship between the Antifungal Susceptibility Profile and the Production of Virulence-Related Hydrolytic Enzymes in Brazilian Clinical Strains ofCandida glabrata

Evaluation of Micronuclei, Nuclear Anomalies and the Nuclear/Cytoplasmic Ratio of Exfoliated Cervical Epithelial Cells in Genital Candidiasis

Anti-Immune Strategies of Pathogenic Fungi

Contact Info

Product

Resources

About