The overexpression of OsSRO1a, which encodes an OsNINJA1- and OsMYC2-interacting protein, negatively affects OsMYC2-mediated jasmonate signaling in rice

Kashihara, Keita; Onohata, Tomonori; Yariuchi, Rina; Tanaka, Suzumi; Akimitsu, Kazuya; Gomi, Kenji

doi:10.1007/s00299-019-02504-z

Cited by 15 publications

(13 citation statements)

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“…OsPrx26 is a putative class III peroxidase. Some class III peroxidases are thought to catalyze the polymerization of lignin precursors, such as monolignols [31], and lignin is known to play an important role in Xoo resistance [18,25]. Thus, we measured lignin content and found that lignin levels were significantly higher in osmed25 mutants than in WT plants (Figure 2c).…”

Section: Expression Of Ja-responsive Defense-related Genes In Osmed25...mentioning

confidence: 93%

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The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Suzuki

Fukuda

Lucob-Agustin

et al. 2022

Plants

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Rice bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most severe diseases of rice. However, the regulatory mechanisms of rice defense against Xoo remain poorly understood. The rice MEDIATOR25, OsMED25—a subunit of the mediator multiprotein complex that acts as a universal adaptor between transcription factors (TFs) and RNA polymerase II—plays an important role in jasmonic acid (JA)-mediated lateral root development in rice. In this study, we found that OsMED25 also plays an important role in JA- and auxin-mediated resistance responses against rice bacterial blight. The osmed25 loss-of-function mutant exhibited high resistance to Xoo. The expression of JA-responsive defense-related genes regulated by OsMYC2, which is a positive TF in JA signaling, was downregulated in osmed25 mutants. Conversely, expression of some OsMYC2-independent JA-responsive defense-related genes was upregulated in osmed25 mutants. Furthermore, OsMED25 interacted with some AUXIN RESPONSE FACTORS (OsARFs) that regulate auxin signaling, whereas the mutated osmed25 protein did not interact with the OsARFs. The expression of auxin-responsive genes was downregulated in osmed25 mutants, and auxin-induced susceptibility to Xoo was not observed in osmed25 mutants. These results indicate that OsMED25 plays an important role in the stable regulation of JA- and auxin-mediated signaling in rice defense response.

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Section: Expression Of Ja-responsive Defense-related Genes In Osmed25...mentioning

confidence: 93%

“…OsNINJA1-overexpressing rice plants are more susceptible to Xoo, due to the downregulation of OsMYC2-responsive defense-related genes [23]. The OsNINJA1-interacting protein OsSRO1a also acts as a mediator that suppresses OsMYC2-mediated defense response to Xoo in rice [25].…”

Section: Introductionmentioning

confidence: 99%

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Suzuki

Fukuda

Lucob-Agustin

et al. 2022

Plants

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“…Recently, the OsSRO1c / BOC1 allele in common wild rice was found to reduce callus browning in indica rice transformation through decreasing cell senescence and death caused by oxidative stress [ 17 ]. OsSRO1a, another rice SRO member, acts as a negative regulator of JA signaling by acting as a mediator between OsMYC2 and the OsNINJA1-OsJAZ complex [ 18 ]. A wheat SRO protein Ta-sro1, which has PARP activity and DNA binding ability, was found to take part in seedling vigor and tolerance to salt stress through regulating redox homeostasis and maintaining genomic stability [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of SRO Gene Family in Sesamum indicum (L.) Reveals Its Association with Abiotic Stress Responses

Liu

Wei

Zhou

et al. 2021

IJMS

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SIMILAR TO RCD-ONEs (SROs) comprise a small plant-specific gene family which play important roles in regulating numerous growth and developmental processes and responses to environmental stresses. However, knowledge of SROs in sesame (Sesamum indicum L.) is limited. In this study, four SRO genes were identified in the sesame genome. Phylogenetic analysis showed that 64 SROs from 10 plant species were divided into two groups (Group I and II). Transcriptome data revealed different expression patterns of SiSROs over various tissues. Expression analysis showed that Group II SROs, especially SiSRO2b, exhibited a stronger response to various abiotic stresses and phytohormones than those in Group I, implying their crucial roles in response to environmental stimulus and hormone signals. In addition, the co-expression network and protein-protein interaction network indicated that SiSROs are associated with a wide range of stress responses. Moreover, transgenic yeast harboring SiSRO2b showed improved tolerance to salt, osmotic and oxidative stress, indicating SiSRO2b could confer multiple tolerances to transgenic yeast. Taken together, this study not only lays a foundation for further functional dissection of the SiSRO gene family, but also provides valuable gene candidates for genetic improvement of abiotic stress tolerance in sesame.

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“…These AS events-occurred O. sativa CDEGs encode proteins containing diverse domains such as Pkinase (PF00069), 14_3_3 (PF00244), TPMT (PF05724), zf-U1 (PF06220), Homeobox (PF00046), adh_short (PF00106), and RST (PF12174). Seven of these CDEGs were annotated as “unknown function.” Interestingly, LOC_Os10g42710 ( OsSRO1a ), with an RST domain, was found to suppress Xanthomonas oryzae pv oryzae ( Xoo ) infection through the OsMYC2 -mediated JA signaling pathway ( Kashihara et al, 2020 ). There are seven transcripts of LOC_Os10g42710 ( LOC_Os10g42710.1 to LOC_Os10g42710.7 ) that were detected based on in silico prediction ( Supplementary Figure 5A ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Differentially Expressed Genes Reveal Conserved Mechanisms in the Rice-Magnaporthe oryzae Interaction

Liang

et al. 2022

Front. Plant Sci.

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Magnaporthe oryzae causes rice blast disease and is responsible for major losses in rice production worldwide. Although numerous studies have focused on the interactions between Oryza sativa and M. oryzae, to date, the conserved mechanisms remain in part unclear. In this study, a comparative analysis of transcriptomes of O. sativa L. ssp. japonica cv. ‘Nipponbare’ interacting with three M. oryzae strains (248, 235, and 163) were performed to explore the conserved molecular mechanisms. Differentially expressed genes with similar expression patterns in the interactions between cultivar ‘Nipponbare’ and three M. oryzae strains were defined as Conserved Differentially Expressed Genes (CDEGs). These included 3,647 O. sativa CDEGs and 3,655 M. oryzae CDEGs. Four rice CDEGs (LOC_Os03g19270, LOC_Os07g36600, LOC_Os05g28740, and LOC_Os01g32780) encoding universal stress protein (USP) were induced within 24 h post-inoculation (hpi) by three M. oryzae strains. Meanwhile, overexpression of LOC_Os07g36600 resulted in enhanced rice resistance against M. oryzae. Furthermore, four rice genes coding light-harvesting chlorophyll a/b-binding (LHC) protein (LOC_Os02g52650, LOC_Os09g12540, LOC_Os11g13850, LOC_Os05g22730) were also identified as CDEGs and were induced at 48 hpi, which might contribute to blast resistance through reactive oxygen species (ROS) accumulation. MoCDIP4 is M. oryzae effector inducing rice cell death and were verified that include AA9 CAZy domain (namely GH61 domain). In this study, we found seven MoCDIP4-homologous genes coding proteins with signal peptides and AA9 CAZy domains, which were continuously up-regulated across all infection stages relative to uninoculated control. This study uncovered that genes are required for conserved mechanisms of rice-M. oryzae interaction, which includes rice genes encoding USP proteins and LHC proteins, as well as M. oryzae genes encoding AA9 proteins. This study will help us to understand how O. sativa responds to M. oryzae infections and the molecular mechanisms of M. oryzae pathogenicity.

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The overexpression of OsSRO1a, which encodes an OsNINJA1- and OsMYC2-interacting protein, negatively affects OsMYC2-mediated jasmonate signaling in rice

Cited by 15 publications

References 61 publications

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Comprehensive Analysis of SRO Gene Family in Sesamum indicum (L.) Reveals Its Association with Abiotic Stress Responses

Identification of Differentially Expressed Genes Reveal Conserved Mechanisms in the Rice-Magnaporthe oryzae Interaction

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