Identification of Differentially Expressed Genes Reveal Conserved Mechanisms in the Rice-Magnaporthe oryzae Interaction

Liang, Dong; Qi, Zhongqiang; Du, Yan; Yu, Junjie; Yu, Mina; Zhang, Rongsheng; Cao, Huijuan; X, Pan; Qiao, Junqing; Song, Tianqiao; Liu, Youzhou; Chen, Zhiyi; Liu, Yongfeng

doi:10.3389/fpls.2022.723356

Cited by 7 publications

(5 citation statements)

References 60 publications

(62 reference statements)

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“…To analyze the expression patterns of O. sativa japonica group NRP genes ( OsNRPs ) during M. oryzae infection, we calculated expression values using the data from our previously RNA sequencing (RNA-seq) [ 28 ]. In that study, O. sativa L. ssp.…”

Section: Resultsmentioning

confidence: 99%

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Genome-Wide Prediction and Analysis of Oryza Species NRP Genes in Rice Blast Resistance

Liang

Song

et al. 2022

IJMS

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Members of the N-rich proteins (NRPs) gene family play important roles in the plant endoplasmic reticulum stress in response, which can be triggered by plant pathogens’ infection. Previous studies of the NRP gene family have been limited to only a few plants, such as soybean and Arabidopsis thaliana. Thus, their evolutionary characteristics in the Oryza species and biological functions in rice defense against the pathogenic fungus Magnaporthe oryzae have remained unexplored. In the present study, we demonstrated that the NRP genes family may have originated in the early stages of plant evolution, and that they have been strongly conserved during the evolution of the Oryza species. Domain organization of NRPs was found to be highly conserved within but not between subgroups. OsNRP1, an NRP gene in the Oryza sativa japonica group, was specifically up-regulated during the early stages of rice-M. oryzae interactions-inhibited M. oryzae infection. Predicted protein-protein interaction networks and transcription-factor binding sites revealed a candidate interactor, bZIP50, which may be involved in OsNRP1-mediated rice resistance against M. oryzae infection. Taken together, our results established a basis for future studies of the NRP gene family and provided molecular insights into rice immune responses to M. oryzae.

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Section: Resultsmentioning

confidence: 99%

“…OsNRP1 was found that specifically up-regulated at an early stage during O. sativa japonica group interacting with three M. oryzae strains proposed by [ 28 ]. Inoculation assays of overexpression transgenic line ( OsNRP1 OX ) revealed that OsNRP1 slowed rice disease.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Prediction and Analysis of Oryza Species NRP Genes in Rice Blast Resistance

Liang

Song

et al. 2022

IJMS

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“…Genes with a combination of P value < 0.01 and the absolute value of |Log 2 fold change|≥ 1.5 were regarded as DEGs. For grouping DEGs with similar expression patterns, a hierarchical clustering was generated using the expression values from each library (Liang et al 2022). Analysis was conducted using Cluster 3.0 software with Pearson correlation as the distance measure.…”

Section: Identification Of Degs Of M Oryzae At Infection Stagementioning

confidence: 99%

Transcriptome and differential expression analysis revealed the pathogenic-related genes in Magnaporthe oryzae during leaf and panicle infection

Du,

Liang,

et al. 2024

Phytopathol Res

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Magnaporthe oryzae is one of the most destructive pathogens that threaten rice production around the world. Previous studies mainly focus on pathogenic mechanism of M. oryzae during infection on rice at leaf stage. However, the pathogenic mechanism of M. oryzae infection on panicle tissue is not well understood. In the present study, we performed RNA sequencing (RNA-seq) to study gene expression patterns of M. oryzae during infection at leaf stage and at panicle stage, respectively. The differentially expressed genes (DEGs) of M. oryzae in the infected leaf and panicle tissues were analyzed. Gene ontology (GO) enrichment analysis of DEGs revealed that M. oryzae genes involved in the biological processes were different at leaf and panicle stages. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DEGs indicates that genes related to individual and important pathways may function at different infection stages. In particular, CAZymes carbohydrate esterases (CEs), carbohydrate-binding modules (CBMs), and glycoside hydrolases (GHs) may play important roles during M. oryzae infection on rice leaves, while glycosyltransferases (GTs) and GHs may play important roles during infection at rice panicle stage. Further analysis of effectors (BAS3, BAS113, BAS162, MoCDIP4, and MoHEG13) and their homologous genes suggest that they are involved in host defense suppression. Our findings provide insights into understanding the infection mechanisms of M. oryzae for rice leaf blast and panicle blast disease.

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“…Previous studies demonstrated that the infection of M. oryzae induced the expression of many host genes [17][18][19] , including transcription factor genes 20,21 . Although AP2/ERFs were known as a transcription factor family responsible for the numerous abiotic stresses in plants 22 , they were shown to play crucial roles in the regulation of biotic stress responses 4,23 .…”

Section: Design Of Sgrnasmentioning

confidence: 99%

Designing CRISPR/Cas9 System Targeting OsERF922 Gene of J02 Rice Variety

Bui¹,

Dong²,

Le³

et al. 2023

Asian J. of Plant Sciences

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Background and Objective: AP2/ERF has been known as a key transcription factor that involves biotic stress responses in rice (Oryza sativa). However, little information is available on the involvement of AP2/ERF in response to rice blast disease. In this study, the expression level of the rice OsERF922 gene that encodes AP2/ERF was analyzed in the J02 variety treated with Magnaporthe oryzae, the causal agent of rice blast disease and a CRISPR/Cas9 targeting the OsERF922 was engineered for further study the functions of OsERF922. Materials and Methods: The expression of OsERF922 in rice was measured by Reverse Transcription PCR (RT-PCR). The Single Guide RNAs (sgRNAs) targeting OsERF922 were designed by CRISPR-P 2.0 software and constructed into a pENTR4 vector. Results: RT-PCR analysis revealed that the expression of OsERF922 of the treated plants was up-regulated compared to those in controls. In addition, two sgRNA sequences targeting OsERF922 were successfully cloned into the expression vector. Conclusion: The results provided useful materials for further functional characterization of OsERF922 by the CRISPR/Cas9 system.

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Identification of Differentially Expressed Genes Reveal Conserved Mechanisms in the Rice-Magnaporthe oryzae Interaction

Cited by 7 publications

References 60 publications

Genome-Wide Prediction and Analysis of Oryza Species NRP Genes in Rice Blast Resistance

Genome-Wide Prediction and Analysis of Oryza Species NRP Genes in Rice Blast Resistance

Transcriptome and differential expression analysis revealed the pathogenic-related genes in Magnaporthe oryzae during leaf and panicle infection

Designing CRISPR/Cas9 System Targeting OsERF922 Gene of J02 Rice Variety

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