The osteoimmunomodulatory properties of MBG scaffold coated with amino functional groups

Abstract

Mesoporous bioactive glass (MBG) is a good scaffold for bone regeneration. In this study, amino functionalized MBG (N-MBG) was used as a model scaffold to examine the effect of the scaffold to bone marrow stromal cells (BMSCs) and macrophages. The MTT results revealed that the proliferation of BMSCs from ovariectomized rabbits was enhanced by N-MBG. Compared to the control group, the expression of osteogenic genes was significantly enhanced by N-MBG, which was related to CaSR pathway. Meanwhile, the anti-inflammatory cytokines (interleukin-10 and arginase-1) were also upregulated by N-MBG stimulation compared with MBG. Furthermore, the amino functionalization of MBG resulted in an increase in the pH value of the material extract. Interestingly, the formation of TRAP multinuclear cells was inhibited by the slightly alkaline extract to a certain extent, which reasonably explained the increase in TRAP multinuclear cells after adjusting the pH value of N-MBG extract. In vivo, the areas of new bone formation in the maxillary sinus floor elevation were increased in the N-MBG/BMSCs group with less TRAP multinuclear cells compared with the MBG/BMSCs group. These findings provided valuable insight that the osteogenic ability of MBG scaffold could be enhanced by amino functionalization due to coordinate BMSCs and macrophages differentiation.

Advanced smart biomaterials and constructs for hard tissue engineering and regeneration

“…In addition, an amino-functionalized bioactive glass (MBG) scaffold was developed to investigate its effects on bone marrow mesenchymal stem cells (BMSCs) and macrophages. 35 The osteogenic ability of the MBG scaffold was enhanced via amino functionalization and could coordinate BMSCs and macrophage differentiation. The osteoimmunomodulatory capability of the scaffold was endowed by the amino functionalization.…”

“…The osteoimmunomodulatory capability of the scaffold was endowed by the amino functionalization. 35 Furthermore, β-tricalcium phosphate (β-TCP), with reported favourable osteoimmunomodulatory properties, was used to coat Mg scaffolds to modulate the detrimental osteoimmunomodulatory properties of Mg scaffolds. 36 β-TCP-coated Mg scaffolds induced macrophages to switch to the extreme M2 phenotype, which caused the release of anti-inflammatory cytokines and osteoinductive molecules by the macrophages.…”

The State of the Art and Prospects for Osteoimmunomodulatory Biomaterials

“…The following section synthesizes the recent advancements in the field of immunomodulatory biomaterials for osteogenesis and osteoclastogenesis, with a special focus on the tunable properties of the bone biomaterials. Porosity/pore size larger pore size: ↓ inflammation, ↑ angiogenic process; [178][179][180][181][182] Delivery of biological molecules elicit immunoregulatory effects [2,[183][184][185][186][187][188][189][190][191][192][193][194][195][196][197] Data are collected from the specialized literature as seen in the flowchart from Figure S1 (Supplementary Materials). ↑ indicates enhancement; ↓ indicates inhibition.…”

Mesoporous Bioactive Glass Nanoparticles Promote Odontogenesis and Neutralize Pathophysiological Acidic pH

“…The HCA layer creates a strong bond between BG and hard tissues. BG-based biomaterials are biocompatible, angiogenic, immunomodulatory, antibacterial, and anti-inflammatory (Kargozar et al, 2018;Zeng et al, 2018;Zhou et al, 2018;Lin et al, 2019). Since BG is relatively low cost biomaterial, it possesses the potential to be the cost effective biomaterial for pulp-dental regeneration.…”