The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures

Mangano, Carlo; Rosa, Alfredo De; Desiderio, Vincenzo; d’Aquino, Riccardo; Piattelli, Adriano; Francesco, Francesco De; Tirino, Virginia; Mangano, Francesco; Papaccio, Gianpaolo

doi:10.1016/j.biomaterials.2010.01.056

Cited by 129 publications

(109 citation statements)

References 25 publications

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“…Several studies have shown that SLA not only enhanced the attachment and differentiation of osteoblast, MG63 and bone marrowderived cells, but also led to increased bone apposition in vivo ( Buser D, 2001;Lumbikanonda et al, 2001;Boyan et al, 2003;Karageorgiou et al, 2005;Kim et al, 2006;Shibli et al, 2007;Ricci et al, 2008). Furthermore, a recent study with stem cells derived from the dental pulp has reported better and quicker osteogenic differentiation when the cells were challenged with even rougher, laser-sintered titanium surface vs. just acid-etched titanium (Mangano et al, 2010). Interestingly, Wall et al, 2009 showed that similarly to our data, MSC from the bone marrow proliferated better on smooth titanium but nevertheless showed an increased matrix mineralization on rough titanium.…”

Section: Discussionmentioning

confidence: 99%

Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

Docheva¹,

Padula²,

Popov³

et al. 2010

eCM

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Periodontal ligament (PDL) can be obtained from patients undergoing orthodontic treatment. PDL contains progenitor cells that can be expanded and differentiated towards several mesenchymal lineages in vitro. Furthermore, PDL-derived cells have been shown to generate bone-and PDL-like structures in vivo. Thus, PDL cells, combined with suitable biomaterials, represent a promising tool for periodontitisrelated research and PDL engineering.Here, a new PDL cell line using lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) was created. HTERT-expressing PDL cells showed similar morphology and population doubling time but an extended lifespan compared to the primary cells. In addition, PDLhTERT cells expressed several characteristic genes and upon osteogenic stimulation produced a calcified matrix in vitro. When cultivated on two topographically different titanium scaffolds (MA and SLA), PDL-hTERT cells exhibited augmented spreading, survival and differentiation on smooth (MA) compared to rough (SLA) surfaces. These findings differ from previously reported osteoblast behaviour, but they are in agreement with the behaviour of chondrocytes and gingival fibroblasts, suggesting a very cell type-specific response to different surface textures.In summary, we report the testing of titanium biomaterials using a new PDL-hTERT cell line and propose this cell line as a useful model system for periodontitis research and development of novel strategies for PDL engineering.

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Section: Discussionmentioning

confidence: 99%

Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

Docheva¹,

Padula²,

Popov³

et al. 2010

eCM

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“…Mangano et al seeded human dental pulp stem cells on direct laser metal sintered titanium scaffolds and acid etched surfaces. They observed that gene expression and protein secretion were faster on laser sintered scaffolds [86]. Another comparative study has been proposed between porous and non-porous titanium alloy Ti-6Al-4V samples.…”

Section: Biological Interaction and Porous Surface Geometrymentioning

confidence: 99%

Porous Titanium for Dental Implant Applications

Wally

Grunsven

Claeyssens

et al. 2015

Metals

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Abstract:Recently, an increasing amount of research has focused on the biological and mechanical behavior of highly porous structures of metallic biomaterials, as implant materials for dental implants. Particularly, pure titanium and its alloys are typically used due to their outstanding mechanical and biological properties. However, these materials have high stiffness (Young's modulus) in comparison to that of the host bone, which necessitates careful implant design to ensure appropriate distribution of stresses to the adjoining bone, to avoid stress-shielding or overloading, both of which lead to bone resorption. Additionally, many coating and roughening techniques are used to improve cell and bone-bonding to the implant surface. To date, several studies have revealed that porous geometry may be a promising alternative to bulk structures for dental implant applications. This review aims to summarize the evidence in the literature for the importance of porosity in the integration of dental implants with bone tissue and the different fabrication methods currently being investigated. In particular, additive manufacturing shows promise as a technique to control pore size and shape for optimum biological properties. OPEN ACCESSMetals 2015, 5 1903

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“…A stable and sufficiently stiff and resistant interface between the biomaterial surface and the surrounding tissue is a vital prerequisite both for immediate implant loading and for the long-term success of such implants. This phenomenon has been extensively analyzed in the literature [3][4][5][6] . The interface is usually achieved by the biological process (partially regulated by mechanical and material properties) known as osseointegration of the implant, which is obtaining an intimate connection of the implant within the bone by means of an appropriate and sufficient growth of new bone on the surface of the implant 7) .…”

Section: Introductionmentioning

confidence: 99%

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

et al. 2014

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Interaction between cells and implant surface is crucial for clinical success. This interaction and the associated surface treatment are essential for achieving a fast osseointegration process. Several studies of different topographical or chemical surface modifications have been proposed previously in literature. The Biomimetic Advanced Surface (BAS) topography is a combination of a shot blasting and anodizing procedure. Macroroughness, microporosity of titanium oxide and Calcium/Phosphate ion deposition is obtained. Human mesenchymal stem cells (hMCSs) response in vitro to this treatment has been evaluated. The results obtained show an improved adhesion capacity and a higher proliferation rate when hMSCs are cultured on treated surfaces. This biomimetic modification of the titanium surface induces the expression of osteblastic differentiation markers (RUNX2 and Osteopontin) in the absence of any externally provided differentiation factor. As a main conclusion, our biomimetic surface modification could lead to a substantial improvement in osteoinduction in titanium alloy implants.

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The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures

Cited by 129 publications

References 25 publications

Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

Porous Titanium for Dental Implant Applications

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

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