The Origin of Lecithodesmus (Digenea: Campulidae) Based on ND3 Gene Comparison

Fernández, Mercedes; Aznar, Francisco; Raga, J. A.; Latorre, Amparo

doi:10.1645/0022-3395(2000)086[0850:tooldc]2.0.co;2

Cited by 11 publications

(4 citation statements)

References 14 publications

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“…For the family Brachycladiidae, evidence suggests that other host-switching events may have occurred once that initial association with cetaceans was established. Some studies have suggested that baleen whales and pinnipeds acquired brachycladiids from toothed whales, and sea otters from pinnipeds (Fernández et al, 2000;Hoberg and Adams, 2000;Aznar et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Independent host switching events by digenean parasites of cetaceans inferred from ribosomal DNA

Fraija-Fernández

Olson

Crespo

et al. 2015

International Journal for Parasitology

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Section: Discussionmentioning

confidence: 99%

Independent host switching events by digenean parasites of cetaceans inferred from ribosomal DNA

Fraija-Fernández

Olson

Crespo

et al. 2015

International Journal for Parasitology

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“…Genomic DNA was extracted from entire specimens using a Gentra Puregene Cell and Tissue Kit (Qiagen Co., Hilden, Germany) according to the manufacturer's protocol for DNA purification from fixed tissue. Partial fragments of the mtND3 and LSU (D1-D3 regions) rRNA genes were amplified using primer combinations: ND3F (5′-GCT TAA TTK KTA AAG CYT TGR ATT CTT ACT-3′; Fernańdez et al, 2000) + ND3-4 (5′-CTA GTC CCA CTC AAC GTA ACC TT-3′; Fernańdez et al, 1998), and CLF28A (5′-AGT AAC GGC GAG TGA ACA GG-3′) + CLF28B (5′-GCA CTG GTC CGA AGA CTA TG-3′; Nakagun et al, 2018), respectively. The amplification reactions were performed in a total volume of 50 ml comprising 1 (mtND3) or 5 (28S rDNA) ml gDNA, 0.4 mM dNTP, 15 mM of each primer, 0.2 µg/µl of BSA, 2.5 mM MgCl 2 , and 0.5 ml of Taq DNA polymerase.…”

Section: Methodsmentioning

confidence: 99%

Novel morphological and molecular data for Nasitrema spp. (Digenea: Brachycladiidae) in the East Asian finless porpoise (Neophocaena asiaeorientalis sunameri)

Kim

Youn

Lee³

et al. 2023

Front. Mar. Sci.

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The East Asian finless porpoise, Neophocaena asiaeorientalis sunameri, ranks among the most endangered species with rapidly decreasing population in the Northwest Pacific. Trematode parasites of the genus Nasitrema that inhabit the air sinuses, inner ear, and the central nervous system of cetaceans frequently cause equilibrium dysfunction, disorientation, interference with echolocation, incoordination, and nervous system degeneration. Due to their specific location and associated pathologies, they have been recognized as one of the causes of cetacean strandings. Stranding data provides crucial information on the species’ biology, population health, and on the status of entire marine ecosystem. However, published data on parasite-induced standings that include information on the causative parasite pathogens are scarce. As part of a wider survey on the causes of East Asian finless porpoise strandings along the west coast of Korea, herein, we provide novel morphological and molecular data on two sympatric species of Nasitrema, namely, Nasitrema spathulatum and Nasitrema sunameri based on newly collected specimens from a stranded alive East Asian finless porpoise at the West coast of Korea. Our study adds a new distribution record for important parasite pathogens in cetaceans and provides the first molecular data for the parasite species recovered, which enabled us to re-evaluate the species relationships within the family Brachycladiidae, a group of important parasite pathogens of marine mammals.

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“…Genomic DNA of the trematodes were extracted either from specimens fixed and stored in 100% ethanol or from FFPE liver tissue containing the parasite, using the NucleoSpin Tissue kit (MACHEREY-NAGEL, Düren, Germany) following the manufacturer's instructions. PCR and subsequent sequencing of the trematode mitochondrial DNA NADH dehydrogenase subunit 3 (mtND3) sequence was conducted using primers ND3F ( 36 ) and ND3-4 ( 37 ) as described previously ( 26 ). The obtained DNA sequences were analyzed on Sequence Scanner Software, version 2 (Applied Biosystems, California, USA), and aligned on MEGA7, version 7.0.18 with the MUSCLE program.…”

Section: Methodsmentioning

confidence: 99%

Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes

Nakagun

Kobayashi

2020

Front. Vet. Sci.

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Hepatic trematodiasis is a common condition in a number of free-ranging cetacean species, which occasionally result in severe hepatic and/or pancreatic lesions. However, even the basic pathological information of this disease is unknown for the majority of affected species. The current study describes and compares the histomorphology and immune reaction induced by hepatic trematodes of the family Brachycladiidae in the liver of the harbor porpoise (Phocoena phocoena, n = 8), Dall's porpoise (Phocoenoides dalli, n = 8), and Hubbs' beaked whale (Mesoplodon carlhubbsi, n = 2). Immunohistochemistry for eight antibodies (CK19, CD3, Foxp3, CD20, Iba1, CD68, CD163, and CD204) was conducted to analyze the pathology of these parasitic infections. In all three odontocete species, the changes observed in the trematode-affected biliary epithelium were comparable with marked hyperplasia and goblet cell metaplasia, as well as lymphoplasmacytic and eosinophilic inflammation. Additionally, regions of the Glisson's sheath were diffusely and severely fibrotic in all examined species, regardless of the physical presence of trematodes. Differences among the three species included the presence of characteristic lymphoid follicles formed in the fibrotic bile duct walls of only the two porpoise species. In the Hubbs' beaked whale, the degree of lymphoplasmacytic cholangitis was more severe, and ductular reaction was generally more prominent. In terms of the overall macrophage population among the three species, CD163-and CD204-positive cells (M2 macrophages) outnumbered Iba1-and CD68-positive cells (M1 macrophages), indicating a chronic infection stage in all analyzed individuals. Species-specific differences among the infiltrating macrophages included numbers of CD68-positive cells being significantly more abundant in the harbor porpoises, whereas CD163-positive cells were significantly more numerous in the Dall's porpoises. The numbers of CD204-positive macrophages were higher in the Hubbs' beaked whales compared to those in the porpoises. Trematode species of the harbor and Dall's porpoises were Campula oblonga, while they were Oschmarinella macrorchis in the Hubbs' beaked whales. This study concludes that interspecies differences in the tissue reactions to hepatic trematode infections are present among odontocete species and that the immune reaction varies depending on the species. This information aids in furthering our understanding of the pathogenesis of hepatic trematodiasis in cetaceans.

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The Origin of Lecithodesmus (Digenea: Campulidae) Based on ND3 Gene Comparison

Cited by 11 publications

References 14 publications

Independent host switching events by digenean parasites of cetaceans inferred from ribosomal DNA

Independent host switching events by digenean parasites of cetaceans inferred from ribosomal DNA

Novel morphological and molecular data for Nasitrema spp. (Digenea: Brachycladiidae) in the East Asian finless porpoise (Neophocaena asiaeorientalis sunameri)

Histochemical and Immunohistochemical Characterizations of Hepatic Trematodiasis in Odontocetes

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