Independent host switching events by digenean parasites of cetaceans inferred from ribosomal DNA

Fraija-Fernández, Natalia; Olson, Peter D.; Crespo, Enrique A.; Raga, Juan Antonio; Aznar, Francisco Javier; Fernández, Mercedes

doi:10.1016/j.ijpara.2014.10.004

Cited by 46 publications

(28 citation statements)

References 58 publications

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“…In all phylogenetic trees, different species of Haplorchis clustered together in a single clade. This agrees with the reports on phylogenetic relationships of heterophyid trematodes based on ITS2 sequences (Thaenkham et al 2012;Fraija-Fernández et al 2015;Sripalwit et al 2015). The phylogenetic trees showed that S. tridactyla clusters with the Haplorchis group of Heterophyidae.…”

Section: Discussionsupporting

confidence: 91%

Molecular characterization of Stictodora tridactyla (Trematoda: Heterophyidae) from Kuwait Bay using rDNA ITS and mtCO1

et al. 2015

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Stictodora tridactyla is an intestinal fluke in the family Heterophyidae that parasitizes shorebirds and mammals, including humans. Its metacercarial cyst stage was reported in the Arabian killifish, Aphanius dispar, at Kuwait Bay. In the present study, Cerithidea cingulata was found to serve as the first intermediate host of S. tridactyla. In order to establish the snail-fish link in the life cycle of S. tridactyla, complete sequences of ribosomal DNA internal transcribed spacer region 1 and 2 (rDNA ITS1 and ITS2) and partial sequence of cytochrome oxidase subunit 1 were obtained for metacercarial cysts isolated from the fish A. dispar and rediae isolated from the snail C. cingulata. Sequence alignment demonstrated that these larval stages belong to the same heterophyid species, S. tridactyla. Phylogenetic analysis based on rDNA ITS1, ITS2, and mtCO1 confirmed the position of S. tridactyla within the Heterophyidae and found it to cluster with Haplorchis spp. The present study represents the first molecular study correlating the larval stages of S. tridactyla using rDNA ITS1, ITS2, and mtCO1 and examining the phylogenetic relationships of S. tridactyla with different heterophyid species.

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Section: Discussionsupporting

confidence: 91%

Molecular characterization of Stictodora tridactyla (Trematoda: Heterophyidae) from Kuwait Bay using rDNA ITS and mtCO1

et al. 2015

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“…A recent phylogenetic study has confirmed that this species belongs to the family Heterophyidae [17]. Pholeter gastrophilus is distributed in the North and South Atlantic Ocean, as well as in the Mediterranean, Black and North Seas (S1 Fig).…”

Section: Introductionmentioning

confidence: 81%

“…It has been hypothesized that the ancestor of P . gastrophilus colonised cetaceans from marine birds via trophic guilds [17]. Since colonization via host switching should start from a small population, the population of P .…”

Section: Discussionmentioning

confidence: 99%

“…gastrophilus , and sequences from Pygidiopsis genata and Ascocotyle longa (Heterophyidae), which were downloaded from GenBank (accession numbers AY245710 and AY245703, respectively) and used as outgroups. Outgroups were selected according to previous phylogenetic studies for the family Heterophyidae [17]. Sequences were aligned using the online version of MAFFT, available at http://mafft.cbrc.jp/alignment/server/.…”

Section: Methodsmentioning

confidence: 99%

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Long-Distance Travellers: Phylogeography of a Generalist Parasite, Pholeter gastrophilus, from Cetaceans

et al. 2017

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We studied the phylogeography and historical demography of the most generalist digenean from cetaceans, Pholeter gastrophilus, exploring the effects of isolation by distance, ecological barriers and hosts’ dispersal ability on the population structure of this parasite. The ITS2 rDNA, and the mitochondrial COI and ND1 from 68 individual parasites were analysed. Worms were collected from seven oceanic and coastal cetacean species from the south western Atlantic (SWA), central eastern Atlantic, north eastern Atlantic (NEA), and Mediterranean Sea. Pholeter gastrophilus was considered a single lineage because reciprocal monophyly was not detected in the ML cladogram of all individuals, and sequence variability was <1% for mtDNA and 0% for ITS2. These results rule out a recent suggestion that P. gastrophilus would actually be a cryptic-species complex. The genetic cohesion of P. gastrophilus could rely on the extensive exploitation of wide-ranging and highly mobile cetaceans, with a putative secondary role, if any, of intermediate hosts. Unique haplotypes were detected in SWA and NEA, and an AMOVA revealed significant population structure associated to the genetic variation in these regions. The Equator possibly acts as a significant geographical barrier for cetacean movements, possibly limiting gene flow between northern and southern populations of P. gastrophilus. A partial Mantel tests revealed that the significant isolation of NEA populations resulted from geographic clustering. Apparently, the limited mobility of cetaceans used by P. gastrophilus as definitive hosts in this region, coupled with oceanographic barriers and a patchy distribution of potential intermediate hosts could contribute to significant ecological isolation of P. gastrophilus in NEA. Rather unexpectedly, no genetic differentiation was found in the Mediterranean samples of this parasite. Historical demographic analyses suggested a recent population expansion of P. gastrophilus in the Atlantic Ocean, perhaps linked to initial association and subsequent spreading in cetaceans.

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“…The genus Braunina is a unique group of diplostomoideans parasitizing cetaceans as adults and is the only genus of the family Brauninidae (Niewiadomska, ). Braunina was first included in a molecular phylogenetic analysis by Fraija‐Fernández et al (); however, no cyathocotylids were included in their analysis. Recent studies by Hernández‐Mena et al () and Blasco‐Costa and Locke () called the systematic position of the Brauninidae in relation to the Cyathocotylidae into question.…”

Section: Discussionmentioning

confidence: 99%

Molecular phylogeny of the Cyathocotylidae (Digenea, Diplostomoidea) necessitates systematic changes and reveals a history of host and environment switches

et al. 2019

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The Cyathocotylidae is a globally distributed family of digeneans parasitic as adults in fish, reptiles, birds and mammals in both freshwater and marine environments. Molecular phylogenetic analysis of interrelationships among cyathocotylids is lacking with only a few species included in previous studies. We used sequences of the nuclear 28S rRNA gene to examine phylogenetic affinities of 11 newly sequenced taxa of cyathocotylids and the closely related family Brauninidae collected from fish, reptiles, birds and dolphins from Australia, Southeast Asia, Europe, North America and South America. This is the first study to provide sequence data from adult cyathocotylids parasitic in fish and reptiles. Our analyses demonstrated that the members of the genus Braunina (family Brauninidae) belong to the Cyathocotylidae, placing the Brauninidae into synonymy with the Cyathocotylidae. In addition, our DNA sequences supported the presence of a second species in the currently monotypic Braunina. Our phylogeny revealed that Cyathocotyle spp. from crocodilians belong to a separate genus (Suchocyathocotyle, previously proposed as a subgenus) and subfamily (Suchocyathocotylinae subfam. n.). Morphological study of Gogatea serpentum indicum supported its elevation to species as Gogatea mehri. The phylogeny did not support Holostephanoides within the subfamily Cyathocotylinae; instead, Holostephanoides formed a strongly supported clade with members of the subfamily Szidatiinae (Gogatea and Neogogatea). Therefore, we transfer Holostephanoides into the Szidatiinae. DNA sequence data revealed the potential presence of cryptic species reported under the name Mesostephanus microbursa. Our phylogeny indicated at least two major host switching events in the evolutionary history of the subfamily Szidatiinae which likely resulted in the transition of these parasites from birds to fish and snakes. Likewise, the transition to dolphins by Braunina represents another major host switching event among the Cyathocotylidae. In addition, our phylogeny revealed more than a single transition between freshwater and marine environments demonstrated in our dataset by Braunina and some Mesostephanus.

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Independent host switching events by digenean parasites of cetaceans inferred from ribosomal DNA

Cited by 46 publications

References 58 publications

Molecular characterization of Stictodora tridactyla (Trematoda: Heterophyidae) from Kuwait Bay using rDNA ITS and mtCO1

Molecular characterization of Stictodora tridactyla (Trematoda: Heterophyidae) from Kuwait Bay using rDNA ITS and mtCO1

Long-Distance Travellers: Phylogeography of a Generalist Parasite, Pholeter gastrophilus, from Cetaceans

Molecular phylogeny of the Cyathocotylidae (Digenea, Diplostomoidea) necessitates systematic changes and reveals a history of host and environment switches

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