The Organochlorine Pesticide Lindane Interacts with the Human Erythrocyte Membrane

Suwalsky, Mario; Rodríguez, Carola Gómez; Villena, Fernando Pardo-Manuel de; Aguilar, Felipe; Sotomayor, Carlos P.

doi:10.1006/pest.1998.2374

Cited by 17 publications

(1 citation statement)

References 31 publications

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“…Such homeoviscous adaptations were intensively studied in connection with thermal acclimation (Williams & Hazel, 1994) but also as a result contaminant exposure. Many investigators showed that fluidity of membrane lipids are affected by contaminants like lindane (Suwalsky et al, 1998), α-and ß-endosulfan (Videira et al, 1999), dichlorodiphenyltrichloroethane (DDT) (Nelson, 1987), polycyclic aromatic hydrocarbons (PAH) (Engelke et al, 1996;Jimenez et al, 2002), and perfluorooctane sulfonate (PFOS) (Hu et al, 2003). Several studies demonstrated that components in crude oil can induce changes in the lipid composition of fish, by decreasing the ratio of polyunsaturated fatty acids (PUFA) to saturated fatty acids (SFA) (Dey et al, 1983;Hylland et al, 2006;Meier et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Effects of Dietary Exposure of Oil-ContaminatedCalanus finmarchicusin Atlantic Herring (Clupea harengus)

Waagbø

Pedersen

Meier

2011

Journal of Toxicology and Environmental Health, Part A

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Suppression subtractive hybridization (SSH) cDNA library construction and characterization was used to identify differentially regulated transcripts from oil exposure in liver of male Atlantic herring (Clupea harengus) fed a diet containing 900 mg crude oil/kg for 2 mo. In total, 439 expressed sequence tags (EST) were sequenced, 223 from the forward subtracted library (enriched for genes putatively upregulated by oil exposure) and 216 from the reverse subtracted library (enriched for genes putatively downregulated by oil exposure). Follow-up reverse-transcription (RT) quantitative polymerase chain reaction (qPCR) analyses of gene transcription were conducted on additional herring exposed to food containing 9 (low), 90 (medium), and 900 (high) mg crude oil/kg feed for 2 mo. Chronic exposure of Atlantic herring to an oil-contaminated diet mediated upregulation of transcripts encoding antifreeze proteins, proteins in the classical complement pathway (innate immunity), and iron-metabolism proteins. Gene ontology (GO) analysis showed that "cellular response to stress," "regulation to biological quality," "response to abiotic stimuli," and "temperature homeostasis" were the most affected go at the biological processes level, and "carbohydrate binding," "water binding," and "ion binding" at the molecular function level. Of the genes examined with RT-qPCR, CYP1A, antifreeze protein, retinol binding protein 1, deleted in malignant brain tumor 1, and ovary-specific C1q-like factor demonstrated a significant upregulation. Myeloid protein 1, microfibrillar-associated protein 4, WAP65, and pentraxin were downregulated in liver of fish from the high exposure group. In conclusion, this study suggests that 2 mo of oil exposure affected genes encoding proteins involved in temperature homeostasis and possible membrane stability in addition to immune-responsive proteins in Atlantic herring.

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