The organochlorine herbicide chloridazon interacts with cell membranes

Suwalsky, Mario; Benites, Maritza; Villena, Fernando Pardo-Manuel de; Norris, Beryl; Quevedo, L

doi:10.1016/s0742-8413(98)90002-0

Cited by 12 publications

(7 citation statements)

References 17 publications

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“…1). Chloridazon has been shown to interact with cell membranes [8] while metflurazon was found to affect lipid biosynthesis [9]. As LCS-1 and both herbicides are 4-chloro-2-phenylpyridazin-3-one derivatives, it is reasonable to assume that the mode of action of LCS-1 is related to that of chloridazon and metflurazon.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Activity of LCS-1 is not Only due to Inhibition of SOD1

Steverding

Barcelos

2019

Drug Res (Stuttg)

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Background The cytotoxic activity of the pyridazin-3-one derivative LCS-1 was previously suggested to be due to the inhibition of superoxide dismutase 1 (SOD1). However, no direct evidence was provided that LCS-1 inhibits SOD1 within cells. Methods In this study, we investigated the cytotoxic activity of LCS-1 against bloodstream forms of Trypanosoma brucei, a protozoan parasite that does not express copper/zinc-containing SOD1, but an iron-containing superoxide dismutase (FeSOD). Results At 250 µM, LCS-1 did not inhibit the activity of FeSOD in cell lysates of bloodstream forms of T. brucei, confirming that the compound is a specific inhibitor of SOD1. However, LCS-1 displayed substantial trypanocidal activity with a minimum inhibitory concentration of 10 µM and a half-maximal effective concentration of 1.36 µM, indicating that the cytotoxic action of the compound cannot solely be due to inhibition of SOD1. Conclusion The results of this study is an important finding as it shows that LCS-1 has more than one cytotoxic mode of action.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic Activity of LCS-1 is not Only due to Inhibition of SOD1

Steverding

Barcelos

2019

Drug Res (Stuttg)

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“…Moreover, this compound also has a teratogenic effect on embryonic development (Venegas et al 1993), and negative effects upon corneal activity (Norris & Quevedo 1993), which may impair the visual capacity of the animal. Another chemical tested was the herbicide Chloridazon, which determines an inhibition of the bioelectric response of the adrenergic neuroepithelial synapse (Suwalsky et al 1998). In addition, it was discovered that the insecticide Chlorpyrifos Phosphorothioate negatively affects the bioelectric activity of the skin (Suwalsky et al 2003).…”

Section: Morpho-physiologymentioning

confidence: 99%

State of knowledge of the Chilean giant frog (Calyptocephalella gayi)

Mora

Bardi²,

Labra

2021

Gayana (Concepc.)

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Globally, at least 43% of amphibian species are declining, due primarily to habitat destruction and modification, over-exploitation, emerging diseases, and invasive alien species. In Chile there are 60 species of amphibians and of these, 62% are endemic and 73% are in a conservation category, such as the Chilean giant frog (Calyptocephalella gayi), a living fossil classified as vulnerable by the International Union for Conservation of Nature (IUCN). Its decline is associated with over-exploitation for sale of its meat as a gourmet product, together with the "megadrought" experienced by Chile in recent years, and the presence of the pathogen Batrachochytrium dendrobatidis, which is causing amphibian mortalities all over the world. The sustained increase in threats to this frog species requires strategic conservation planning, which should be based on the knowledge of aspects of their basic biology. This led us to search for the available published information on this species, compiling the reachable data on the web from 1927 to 2019. We found 353 publications, and our two main conclusions about the knowledge on this species are that there is a high percentage (43%) of publications focused on studies of the morpho-physiological aspects, and that there is a significant lack of data on its ecology and natural history. We discuss how these deficiencies limit the possibility of implementing adequate management and/or conservation plans for this species, and finally propose areas of study that should provide a solid contribution towards the preservation of this species.

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“…The first is the so-called suicidal pathway: PPs are acquired by the decomposer organisms and partially dehalogenated, but the less halogenated metabolites are much more toxic to the microorganisms than the parent compounds and lead to the death of the decomposer organisms. , The second pathway is connected with the membrane activity of PPs. , Generally, polychlorinated hydrocarbons are more hydrophobic than their hydrogenated counterparts . They can effectively interact with the membrane phospholipids and be retained in the cellular membrane. ,, The incorporation of PPs leads to multiple adverse changes of the membrane structure and function and finally to the death of the cell either on the apoptotic or necrotic way.…”

Section: Introductionmentioning

confidence: 99%

“…27 They can effectively interact with the membrane phospholipids and be retained in the cellular membrane. 26,28,29 The incorporation of PPs leads to multiple adverse changes of the membrane structure and function and finally to the death of the cell either on the apoptotic or necrotic way.…”

Section: Introductionmentioning

confidence: 99%

Effects of Polychlorinated Pesticides and Their Metabolites on Phospholipid Organization in Model Microbial Membranes

et al. 2018

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Polychlorinated pesticides (PPs) were classified as persistent organic pollutants because of their toxicity, limited degradability in the environment, bioaugmentation, and accumulation in animal tissues. PPs accumulate in the environment mainly in the soils and water sediments where they are toxic to the decomposer organisms including soil bacteria and fungi. Therefore, there is an urgent need to search for the microorganisms capable of PP biodegradation which could be applied for soil bioremediation. The exact mechanism of PP microbial toxicity is unknown; however, there is evidence that it can be membrane related. To shed light on the interactions of PPs with microbial membranes, we applied Langmuir monolayers formed by phospholipids as model biomembranes. The model membranes were formed by phospholipids typical to microbial membranes: cardiolipins and phosphatidylglycerols the main components of Gram positive bacteria membranes, phosphatidylcholine typical to fungal membranes, as well as phosphatidylethanolamine found in the inner membranes of Gram negative bacteria. For the studies, the most ecotoxic PPs and their water-soluble metabolites were chosen. The monolayers were studied with the application of mutually complementary techniques: Langmuir technique, grazing incidence X-ray diffraction, and PM-IRRAS spectroscopy. It turned out that the cyclodiene PPs are more membrane active than monocyclic PPs and that the possibility of their incorporation is strictly related to the phospholipid structure. The membranes prepared with cardiolipin turned out to be especially resistant to PP incorporation. Regarding the metabolites, pentachlorophenol turned out to be especially structure breaking, affecting the molecular organization of all of the investigated phospholipids.

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The organochlorine herbicide chloridazon interacts with cell membranes

Cited by 12 publications

References 17 publications

Cytotoxic Activity of LCS-1 is not Only due to Inhibition of SOD1

Cytotoxic Activity of LCS-1 is not Only due to Inhibition of SOD1

State of knowledge of the Chilean giant frog (Calyptocephalella gayi)

Effects of Polychlorinated Pesticides and Their Metabolites on Phospholipid Organization in Model Microbial Membranes

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