The organization and expression of a maize ribosomal protein gene family.

Larkin, John C.; Hunsperger, John P.; Culley, David E.; Rubenstein, Irwin; Silflow, Carolyn D.

doi:10.1101/gad.3.4.500

Cited by 72 publications

(56 citation statements)

References 58 publications

(62 reference statements)

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“…Maize genomic DNA was isolated as described by Larkin et al (1989). DNA (15 pg) was digested with either EcoRI, HindIII, or BamHI and the restriction fragments were sep- predicted from their root-tip cDNAs (ZMANNp33, ZMANNp35) and aligned against predicted annexin sequences for alfalfa (MSANN; Pirck et al, 1994), strawberry (FAANN; Wilkinson et al, 1995), and human annexin Xllla (HSANNXIII; Wice and Cordon, 1992).…”

Section: Southern and Northern Blottingmentioning

confidence: 99%

cDNA Isolation and Gene Expression of the Maize Annexins p33 and p35

1996

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Section: Southern and Northern Blottingmentioning

confidence: 99%

cDNA Isolation and Gene Expression of the Maize Annexins p33 and p35

1996

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“…We isolated cDNA and genomic clones of the Chlamydomonas gene for ribosomal protein S14 (CRY]) by using a degenerate oligonucleotide mixture and an S14 cDNA from maize as hybridization probes (22). The deduced amino acid sequence of the C. reinhardtii S14 protein showed a high degree of similarity to sequences of the previously characterized rp59/ S14 proteins.…”

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confidence: 99%

The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.

Nelson¹,

Savereide²,

Lefebvre³

1994

Mol. Cell. Biol.

135

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We have cloned and sequenced the CRY] gene, encoding ribosomal protein S14 in Chkimydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. We isolated a mutant strain resistant to the eukaryotic translational inhibitors cryptopleurine and emetine in which the resistance was due to a missense mutation (CRY)-I) in the CRY) gene; resistance was dominant in heterozygous stable diploids. Cotransformation experiments using the CRY)-) gene and the gene for nitrate reductase (NIT)) produced a low level of resistance to cryptopleurine and emetine. Resistance levels were increased when the CRY)-) gene was placed under the control of a constitutive promoter from the ribulose bisphosphate carboxylase/oxygenase small subunit 2 (RBCS2) gene. We also found that the 5' untranslated region of the CRY) gene was required for expression of the CRY)-) transgene. Direct selection of emetine-resistant transformants was possible when transformed cells were first induced to differentiate into gametes by nitrogen starvation and then allowed to dedifferentiate back to vegetative cells before emetine selection was applied. With this transformation protocol, the RBCS2/CRYI-I dominant selectable marker gene is a powerful tool for many molecular genetic applications in C. reinhardtii.Chlamydomonas reinhardtii has been an excellent model organism for many areas of research, particularly for the study of flagellar structure and function (25), chloroplast function (1), and cell-cell interactions during mating (9). The development of reliable nuclear transformation procedures (18) opened the Chlamydomonas field to techniques such as insertional mutagenesis (47) and homologous recombination (46). One limitation, however, has been a shortage of selectable marker genes. Heterologous genes are very poorly expressed when transformed into C. reinhardtii. A few reports of selection using heterologous genes have appeared (11,13,38), although no heterologous gene has allowed efficient recovery of transformants. Several C. reinhardtii genes corresponding to auxotrophic mutants, including the genes for nitrate reductase (NITJ) (7,19), argininosuccinate lyase (ARG7) (4), and oxygen-evolving enhancer protein 1 (OEEI) (29), have been cloned and characterized for use as efficient transformation markers. Dominant C. reinhardtii mutations that confer resistance to herbicides and other drugs are promising candidates for providing selectable marker genes. We identified a mutation in the ribosomal protein S14 gene of C. reinhardtii that produced resistance to the eukaryotic translation inhibitors cryptopleurine and emetine, providing an easily clonable selectable marker gene. Mutations in the ribosomal protein S14/rp59 genes of Cricetulus griseus (Chinese hamster ovary cells) and Saccharomyces cerevisiae are responsible for resistance to emetine and cryptopleurine, respectively (31, 37), and these and other S14 genes have been sequenced and found to...

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“…Although the deduced amino acid sequence of At S1lD has 92% similarity with a previously reported Arabidopsis cytosol ribosomal protein S11 (At S11) (Gantt and Thompson, 1990) somal proteins are encoded by multigene families (Larkin et al, 1989;Lebm and Freyssinet, 1991). Therefore, we refer to the present cDNA as At S l l p to distinguish it from the previous clone (Gantt and Thompson, 1990), and we conclude that there are at least two different genes encoding cytosol ribosomal protein S11 in Arabidopsis thaliana.…”

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confidence: 99%

“…Plants contain three types of ribosomes: one located in the cytosol that is very similar to the cytosol ribosomes of other eukaryotes; one located in mitochondria and whose properties are not well defined; and one, located in the plastid, that is remarkably similar to ribosomes found in bacteria (Boyton et al, 1979). To our knowledge, cDNAs encoding plant cytoplasmic ribosomal proteins have been isolated from maize (Larkin et al, 1989;Lebm and Freyssinet, 1991), soybean, and Arabidopsis thaliana (Gantt and Thompson, 1990).…”

mentioning

confidence: 99%