cDNA Isolation and Gene Expression of the Maize Annexins p33 and p35

Immunolocalization of a novel annexin‐like protein encoded by a stress and abscisic acid responsive gene in alfalfa

“…The deduced amino acid sequence of AnnMs2 is shown in Figure 1 in comparison with the amino acid sequences of Medicago sativa (Pirck et al, 1994), Capsicum annuum (Proust et al, 1996), Arabidopsis thaliana (Gidrol et al, 1996), Zea mays p33 and p35 annexins (Battey et al, 1996). AnnMs2 possesses the common primary structure of annexins: a short N-terminal tail and the C-terminal core composed of four repeats of 70-75 amino acids, but it is more difficult to predict the precise position of the repeats than in the case of animal annexins.…”

“…This type of Ca 2ϩ -binding site has been defined on the basis of the crystallographic analysis of annexin I and V (Weng et al, 1993). (M.s;Pirck et al, 1994), Arabidopsis thaliana (A.th;Gidrol et al, 1996), Capsicum annuum (C.an;Proust et al, 1996) and Zea mays (Z.m33 and Z.m35;Battey et al, 1996). The repeat sequences are bold and residues external to the four repeats are in italic.…”

“…(Blackbourn et al, 1992;Boustead et al, 1989;Seals et al, 1994). Recently, a partial annexin cDNA sequence has been obtained from Medicago sativa (Pirck et al, 1994) and strawberry (Wilkinson et al, 1995), and full-length cDNA sequences from Capsicum annuum (Proust et al, 1996), Zea mays (Battey et al, 1996) and Arabidopsis thaliana (Gidrol et al, 1996). The predicted amino acid sequences of plant annexins show a high degree of similarity to each other, although they are distinct from the mammalian annexins.…”

GTPase Activity and Biochemical Characterization of a Recombinant Cotton Fiber Annexin1

“…The partial amino acid sequences obtained from the two peptides showed significant homology with annexins ( Fig. 1) from Arabidopsis (Gidrol et al, 1996), maize (Battey et al, 1996), and cotton fibers . Our experiments demonstrate for the first time, to our knowledge, the presence of the so-called "annexin doublet" in the cotton fibers similar to those observed in other plants (see Clark and Roux, 1995).…”

“…Since then, relatively abundant annexins have been identified and isolated from a number of plants (Smallwood et al, 1990;Blackbourn et al, 1992;Clark et al, 1992;Andrawis et al, 1993). Although the general functions of plant annexins are still in question (for review, see Clark and Roux, 1995;Delmer and Potikha, 1997), recent isolation of partial or complete annexin cDNA clones from higher plants, including monocots (Battey et al, 1996) and dicots (Pirck et al, 1994;Gidrol et al, 1996;Proust et al, 1996;Potikha and Delmer, 1997), provides some insight into the structural characteristics of plant annexins. The endonexin repeats found in animal annexins are also observed in plant annexins; however, it seems that plant annexins contain fewer predicted Ca 2ϩ -binding sites than animal annexins (see Clark and Roux, 1995).…”